Role of Angiopoietin-like 4 on Bone Vascularization in Chickens Exposed to High-altitude Hypoxia

Huang, Shucheng; Wang, M.; Rehman, Mujeeb Ur; Zhang, L.; Tong, Xili; Shen, Yusen; Li, Jiakui

doi:10.1016/j.jcpa.2018.04.007

Cited by 6 publications

(2 citation statements)

References 36 publications

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“…Previous studies have reported that osteoblasts may regulate angiogenesis [48,49], but this phenomenon has not yet been explored at the single-cell level. Interestingly, transcripts for some secreted factors associated with the vascular system (e.g., VCAN and ANGPTL4 [50,51]) were highly expressed in the osteoblast precursors, (Figure 3A). This result suggests that osteoblast precursors may induce angiogenesis concurrently with osteogenesis.…”

Section: Osteoblast Precursors Induce Angiogenesis During Coupling With Osteogenesismentioning

confidence: 99%

Single-cell RNA sequencing deconvolutes the in vivo heterogeneity of human bone marrow-derived mesenchymal stem cells

Wang¹,

Li²,

Yang³

et al. 2021

Int. J. Biol. Sci.

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Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells that have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and the fat in bone marrow. In addition to providing microenvironmental support for hematopoietic processes, BM-MSCs can differentiate into various mesodermal lineages including osteoblast/osteocyte, chondrocyte, and adipocyte that are crucial for bone metabolism. While BM-MSCs have high cell-to-cell heterogeneity in gene expression, the cell subtypes that contribute to this heterogeneity in vivo in humans have not been characterized. To investigate the transcriptional diversity of BM-MSCs, we applied single-cell RNA sequencing (scRNA-seq) on freshly isolated CD271 + BM-derived mononuclear cells (BM-MNCs) from two human subjects. We successfully identified LEPR hi CD45 low BM-MSCs within the CD271 + BM-MNC population, and further codified the BM-MSCs into distinct subpopulations corresponding to the osteogenic, chondrogenic, and adipogenic differentiation trajectories, as well as terminal-stage quiescent cells. Biological functional annotations of the transcriptomes suggest that osteoblast precursors induce angiogenesis coupled with osteogenesis, and chondrocyte precursors have the potential to differentiate into myocytes. We also discovered transcripts for several clusters of differentiation (CD) markers that were either highly expressed (e.g., CD167b, CD91, CD130 and CD118) or absent (e.g., CD74, CD217, CD148 and CD68) in BM-MSCs, representing potential novel markers for human BM-MSC purification. This study is the first systematic in vivo dissection of human BM-MSCs cell subtypes at the single-cell resolution, revealing an insight into the extent of their cellular heterogeneity and roles in maintaining bone homeostasis.

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Section: Osteoblast Precursors Induce Angiogenesis During Coupling With Osteogenesismentioning

confidence: 99%

Single-cell RNA sequencing deconvolutes the in vivo heterogeneity of human bone marrow-derived mesenchymal stem cells

Wang¹,

Li²,

Yang³

et al. 2021

Int. J. Biol. Sci.

View full text Add to dashboard Cite

show abstract

“…Previous studies have reported that osteoblasts may regulate angiogenesis 39,40 , but this phenomenon has yet not been explored on the single-cell level. Interestingly, transcripts for some secreted factors associated with the vascular system (e.g., VCAN and ANGPTL4 41,42 were highly expressed in osteoblast precursors, ( Supplementary Table 1: Sheet 3). This result suggests that osteoblast precursors may induce angiogenesis concurrently with osteogenesis.…”

Section: Osteoblast Precursor Induce Angiogenesis During Coupling Witmentioning

confidence: 99%

Single-cell RNA sequencing deconvolutes thein vivoheterogeneity of human bone marrow-derived mesenchymal stem cells

Wang

Li²,

Yang

et al. 2020

Preprint

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Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells, which have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and the fat found in bone marrow. In addition to providing microenvironmental support for hematopoietic processes, BM-MSCs can differentiate into various mesodermal lineages including osteoblast/osteocyte, chondrocyte, and adipocyte cells that are crucial for bone metabolism. While BM-MSCs have high cell-to-cell heterogeneity in gene expression, the cell subtypes that contribute to this heterogeneity in vivo in humans have not been characterized. To investigate the transcriptional diversity of BM-MSCs, we applied single-cell RNA sequencing (scRNA-seq) on freshly isolated CD271 + BM-derived mononuclear cells (BM-MNCs) from two human subjects. We successfully identified LEPR hi CD45 low BM-MSCs within the CD271 + BM-MNC population, and further codified the BM-MSCs into distinct subpopulations corresponding to the osteogenic, chondrogenic, and adipogenic differentiation trajectories, as well as terminal-stage quiescent cells. Biological functional annotations of transcriptomes suggest that osteoblast precursors may induce angiogenesis coupled with osteogenesis, and chondrocyte precursors may have the potential to differentiate into myocytes. We discovered transcripts for several cluster of differentiation (CD) markers that were highly expressed (e.g., CD167b, CD91, CD130 and CD118) or absent (e.g., CD74, CD217, CD148 and CD68) in BM-MSCs and could be novel markers for human BM-MSC purification. This study is the first systematic in vivo dissection of human BM-MSCs cell subtypes at the single-cell resolution, revealing insight into the extent of their cellular heterogeneity and bone homeostasis.

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HIF-1α upregulation exerts the antagonistic effect against angiogenesis inhibition in manganese deficiency-induced tibial dyschondroplasia of broiler chicks

Jin

Dong

et al. 2022

Vet Res Commun

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Manganese (Mn) is an essential microelement for broiler breeding and its de ciency causes tibial dyschondroplasia (TD). Tibial growth plate (TGP) development and metaphyseal vascularization are crucial for tibia growth in fast-growing broiler chickens, but their roles in Mn de ciency-induced TD in chicks remains unclear. This study was designed to clarify this issue. A total of 36 one-day-old broilers were divided into the control group and Mn-de ciency (Mn-D) group, which were fed with a standard diet (60 mg Mn/kg) and Mn de ciency diet (22 mg Mn/kg) for 42 days, respectively. TGP and proximal tibial metaphysis were collected to perform the related assays. First, Mn de ciency decreased the tibia length and TGP thickness in TD model. Mn de ciency reduced the number of blood vessels in proximal tibial metaphysis by histological analysis. Also, Mn de ciency decreased the mRNA and protein expression levels of vascular endothelial growth factor (VEGF) and macrophage migration inhibitory factor (MIF) in TGP. Moreover, up-regulation of hypoxia-inducible factor-1 alpha (HIF-1α) at both mRNA and protein levels was found in TGP of Mn-D group, suggesting the antagonistic role of HIF-1α against angiogenesis inhibition. Additionally, Mn de ciency enhanced the autophagy related 5 (ATG5) and microtubule associated protein 1 light chain 3 beta (LC3-II) expression levels, but decreased the SQSTM1 (p62) expression levels in TGP, suggesting autophagy activation in this process. Collectively, these data indicate that HIF-1α up-regulation and concurrent autophagy activation exert the protective effect against Mn de ciency-induced angiogenesis inhibition, which may provide a useful guidance to prevent TD in broilers.

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Role of Angiopoietin-like 4 on Bone Vascularization in Chickens Exposed to High-altitude Hypoxia

Cited by 6 publications

References 36 publications

Single-cell RNA sequencing deconvolutes the in vivo heterogeneity of human bone marrow-derived mesenchymal stem cells

Single-cell RNA sequencing deconvolutes the in vivo heterogeneity of human bone marrow-derived mesenchymal stem cells

Single-cell RNA sequencing deconvolutes thein vivoheterogeneity of human bone marrow-derived mesenchymal stem cells

HIF-1α upregulation exerts the antagonistic effect against angiogenesis inhibition in manganese deficiency-induced tibial dyschondroplasia of broiler chicks

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