Role of an Arabidopsis Rab GTPase RabG3b in Pathogen Response and Leaf Senescence

Kwon, Sanghoon; Cho, Hong Joo; Bae, Kisuk; Jung, Jin Hee; Jin, Hak Chul; Park, Ohkmae K.

doi:10.1007/s12374-009-9011-4

Cited by 28 publications

(35 citation statements)

References 57 publications

(61 reference statements)

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“…as well as Arabidopsis (Kwon et al, 2011). We also reported that RabG3b is involved in cell death associated with the fungal pathogen A. brassicicola and infection with the fungal toxin fumonisin B1 (FB1) as well as leaf senescence (Kwon et al, 2009). Here, we extend our work to determine the role of RabG3b and autophagy in immunity-associated HR PCD.…”

supporting

confidence: 55%

“…Affinity-purified recombinant proteins were used to raise the anti-ATG8a antibody in rats. The anti-RabG3b antibody was raised in rabbits as described previously (Kwon et al, 2009). …”

Section: Antibody Preparationmentioning

confidence: 99%

“…The segregation of F2 seeds was confirmed by selection on kanamycin, and F2 plants were genotyped to select crossing lines. Homozygous RabG3bCA atg5-1 crossing lines were confirmed by PCR, as described previously (Kwon et al, 2009;Yoshimoto et al, 2009). …”

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confidence: 99%

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The Rab GTPase RabG3b Positively Regulates Autophagy and Immunity-Associated Hypersensitive Cell Death in Arabidopsis

et al. 2013

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A central component of the plant defense response to pathogens is the hypersensitive response (HR), a form of programmed cell death (PCD). Rapid and localized induction of HR PCD ensures that pathogen invasion is prevented. Autophagy has been implicated in the regulation of HR cell death, but the functional relationship between autophagy and HR PCD and the regulation of these processes during the plant immune response remain controversial. Here, we show that a small GTP-binding protein, RabG3b, plays a positive role in autophagy and promotes HR cell death in response to avirulent bacterial pathogens in Arabidopsis (Arabidopsis thaliana). Transgenic plants overexpressing a constitutively active RabG3b (RabG3bCA) displayed accelerated, unrestricted HR PCD within 1 d of infection, in contrast to the autophagy-defective atg5-1 mutant, which gradually developed chlorotic cell death through uninfected sites over several days. Microscopic analyses showed the accumulation of autophagic structures during HR cell death in RabG3bCA cells. Our results suggest that RabG3b contributes to HR cell death via the activation of autophagy, which plays a positive role in plant immunity-triggered HR PCD.

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supporting

confidence: 55%

“…Affinity-purified recombinant proteins were used to raise the anti-ATG8a antibody in rats. The anti-RabG3b antibody was raised in rabbits as described previously (Kwon et al, 2009). …”

Section: Antibody Preparationmentioning

confidence: 99%

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The Rab GTPase RabG3b Positively Regulates Autophagy and Immunity-Associated Hypersensitive Cell Death in Arabidopsis

et al. 2013

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“…Plant tissues often exhibit varying degrees of resistance depending on their stage of development. The relationship between maturity and disease resistance (age-related resistance, ARR) is well documented in different plantpathogen systems (Kus et al 2002;Yun and Choi 2003;Rusterucci et al 2005;Zeier 2005;Kwon et al 2009). The onset of some ARR forms is associated with flowering, whereas others are correlated with plant age, leaf size, or the synthesis of secondary metabolites or defense proteins .…”

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confidence: 99%

Age-Dependent Variations of Volatile Emissions and Inhibitory Activity Toward Botrytis cinerea and Fusarium oxysporum in Tomato Leaves Treated with Chitosan Oligosaccharide

Zhang

Chen

2009

J. Plant Biol.

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We investigated variations in the level and composition of volatiles emitted by tomato leaves at different ages. Our focus also included their antifungal properties and responses to chitosan oligosaccharide. Based on leaf position, the release of volatiles decreased over time. Young leaves produced high levels of C6-aldehyde, which is mainly composed of hexenal, while the volatiles emitted by more mature leaves largely comprised terpenes, particularly β-phellandrene and caryophyllane. In young upper leaves, the main components (up to 86% of the total) were hexenal, β-phellandrene, and caryophyllane. Their levels decreased steadily over time, from 386.3 μg g −1 fresh weight (FW) in young leaves to 113.2 μg g −1 FW in old tissues. Volatiles emitted from young leaves exhibited the best antifungal activity against spore germination and hyphal growth by Botrytis cinerea and Fusarium oxysporum. Leaves became more susceptible to oligosaccharide treatment with increasing age. When young tissues were exposed to chitosan, we found declines in both the quantity of volatiles and their ability to inhibit fungal growth. Compared with the control, the amount of volatiles from young tissues was 88.4% lower after such treatment. In contrast, contents of volatiles from old and adult leaves were dramatically increased by chitosan oligosaccharide. Likewise, their inhibitory effect was significantly enhanced. Therefore, our results suggest that these volatiles are responsible for antifungal activity and may play a role in age-related resistance by tomato.

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“…Rab7 is regarded as a key regulator in endo-lysosomal trafficking, which mediates the regulated internalization and degradation of nutrient transporters and triggers nutrient starvation that facilitates cell death [61] . In Arabidopsis, overexpression of the Rab7 homolog RabG3b leads to expanded HR upon pathogen infection and accelerated leaf senescence [62] . A wheat Rab7 homolog TaRab7 was identified and observed to be upregulated in response to salt and drought stress, and infection by the stripe rust fungus, indicating that TaRab7 has a role in plant response to various biotic and abiotic stimuli [63] .…”

Section: Signaling Pathwaymentioning

confidence: 99%

New insights in the battle between wheat and Puccinia striiformis

Tang¹,

Wang²,

Cheng³

et al. 2015

Front. Agr. Sci. Eng.

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Wheat stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) poses a great threat to wheat production worldwide. The rapid change in virulence of Pst leads to a loss of resistance in currently resistant wheat cultivars, which results in frequent disease epidemics. Therefore, a major focus is currently placed on investigating the molecular mechanisms underlying this rapid variation of pathogenicity and coevolving wheat resistance. Limited by the lack of a system for stable transformation of Pst and the difficulties in wheat transformation, it is not easy to generate deeper insights into the wheat-Pst interaction using established genetic methods. Nevertheless, considerable effort has been made to unravel the wheat-Pst interaction and significant progress is being made. Histology and cytology have revealed basic details of infection strategies and defense responses during wheat-Pst interactions, identified cellular components involved in wheat-Pst interactions, and have helped to elucidate their role in the infection process or in plant defense responses. Transcriptome and genome sequencing has revealed the molecular features and dynamics of the wheat-Pst pathosystem. Extensive molecular analyses have led to the identification of major components in the wheat resistance response and in Pst virulence. Studies of wheat-Pst interactions have now entered a new phase in which cellular and molecular approaches are being used. This review focuses on the cellular biology of wheat-Pst interactions and integrates the emerging data from molecular analyses with the histocytological observations.

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Role of an Arabidopsis Rab GTPase RabG3b in Pathogen Response and Leaf Senescence

Cited by 28 publications

References 57 publications

The Rab GTPase RabG3b Positively Regulates Autophagy and Immunity-Associated Hypersensitive Cell Death in Arabidopsis

The Rab GTPase RabG3b Positively Regulates Autophagy and Immunity-Associated Hypersensitive Cell Death in Arabidopsis

Age-Dependent Variations of Volatile Emissions and Inhibitory Activity Toward Botrytis cinerea and Fusarium oxysporum in Tomato Leaves Treated with Chitosan Oligosaccharide

New insights in the battle between wheat and Puccinia striiformis

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