Role of alkaline phosphatase in insecticidal action of Cry1Ac against Helicoverpa armigera larvae

Upadhyay, Santosh Kumar; Singh, Pradhyumna Kumar

doi:10.1007/s10529-011-0665-x

Cited by 28 publications

(26 citation statements)

References 43 publications

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“…Angelucci et al [2] have reported seven APN genes in H. armigera, four among them interact with Cry1Ac d-endotoxin. It is noteworthy that APNs have been comprehensively studied as receptors of d-endotoxins [2,44,49,87]. Fitches et al [30] observed the interaction of garlic bulb lectin with the membrane bound alanyl aminopeptidase N of Acyrthosiphon pisum.…”

Section: Cadherin Like Proteinmentioning

confidence: 99%

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Receptors of Garlic (Allium sativum) Lectins and Their Role in Insecticidal Action

Upadhyay

Singh

2012

Protein J

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Garlic (Allium sativum) lectins are promising candidate molecules for the protection against chewing (lepidopteran) as well as sap sucking (homopteran) insect pests. Molecular mechanism of toxicity and interaction of lectins with midgut receptor proteins has been described in many reports. Lectins show its effect right from sensory receptors of mouth parts by disrupting the membrane integrity and food detection ability. Subsequently, enter into the gut lumen and interact with midgut glycosylated proteins like alkaline phosphatase (ALP), aminopeptidase-N (APN), cadherin-like proteins, polycalins, sucrase, symbionin and others. These proteins play critical role in life cycle of insect directly or indirectly. Lectins interfere with the activity of these proteins and causes physiological disorders leading to the death of insects. Lectins further transported across the insect gut, accumulated in various body parts (like haemolymph and ovary) and interact with intracellular proteins like symbionin and cytochrome p450. Binding with cytochrome p450 (which involve in ecdysone synthesis) might interfere in the development of insects, which results in growth retardation and pre-mature death.

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Section: Cadherin Like Proteinmentioning

confidence: 99%

“…ALPs are also a family of proteins and function as a receptor of d-endotoxins in H. armigera [87], M. sexta [58], H. virescens [45,46,51] and others. Glycan mediated binding of ALPs to d-endotoxins has been demonstrated [79].…”

Section: Gpi-anchored Alkaline Phosphatase (Alp)mentioning

confidence: 99%

Receptors of Garlic (Allium sativum) Lectins and Their Role in Insecticidal Action

Upadhyay

Singh

2012

Protein J

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“…The Cry1Ac toxin is most effective against large number of lepidopteran insects due to efficient oligomerization and binding to these receptors particularly with alkaline phosphatase (Bravo et al 2004; Upadhyay and Singh 2011). Whereas, Cry1Ab toxin is shown to bind rapidly with BT-R 1 receptor involving the stimulation of G-protein adenylyl cyclase/PKA signaling pathway for destabilization of the midgut cytoskeleton, ion channel and pore formation via a different mode of action resulting to mortality of lepidopteran insects (Zhang et al 20052006; Kato et al 2006; Padilla et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Transgenic tomato line expressing modified Bacillus thuringiensis cry1Ab gene showing complete resistance to two lepidopteran pests

et al. 2014

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The modified truncated Bt-cry1Ab gene of Bacillus thuringiensis has been used for the development and selection of over expressing transgenic events in a commercially important variety of tomato (Solanum lycopersicum L.) by Agrobacterium-mediated leaf-disc transformation procedure. The integration and inheritance of cry1Ab gene in T0 transgenic plants and their progenies were determined by PCR, RT-PCR and Southern blot hybridization analysis. The toxin expression was monitored by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). The transgenic line Ab25 E, expressing 0.47 ± 0.01% Cry1Ab toxin of total soluble protein (TSP) was finally selected in the T4 generation from the segregating population, showing 100% mortality to the second instar larvae of H. armigera and S. litura and minimal damages to leaves and fruits. Southern blot analysis data revealed single copy introgression of cry1Ab gene in highly-expressing Ab25 E transgenic line and expression of Cry1Ab toxin of molecular mass ~65 kDa was evident in Western blot analyses in transgenic plants of T4, T5 and T6 generation. Receptor binding assay performed with partially purified Cry1Ab protein from Ab25 E transgenic tomato line, confirmed efficient protein-protein interaction of Cry1Ab toxin with receptor(s) of both the insects. The higher level of Cry1Ab toxin (≈ 0.47 ± 0.01% TSP) did not affect the normal in vitro regeneration, plant development and fruit yield in this transgenic line. This high expressing Cry1Ab homozygous transgenic line can be a useful candidate in tomato breeding programmes for introgression of important agronomical traits.Electronic supplementary materialThe online version of this article (doi:10.1186/2193-1801-3-84) contains supplementary material, which is available to authorized users.

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“…In lepidopteran larvae the first binding event of activated Cry1A toxins is proposed to be a low affinity interaction with the highly abundant GPI-anchored-receptors, alkaline phosphatase (ALP) or aminopeptidase-N (APN) 15–18 . This interaction is proposed to concentrate the toxin in proximity to the brush border microvilli membrane of the midgut cells where the toxin binds, in a high affinity interaction, to the cadherin receptor 15 .…”

Section: Introductionmentioning

confidence: 99%

ABCC2 is associated with Bacillus thuringiensis Cry1Ac toxin oligomerization and membrane insertion in diamondback moth

Ocelotl

Sánchez

Gómez

et al. 2017

Sci Rep

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Cry1A insecticidal toxins bind sequentially to different larval gut proteins facilitating oligomerization, membrane insertion and pore formation. Cry1Ac interaction with cadherin triggers oligomerization. However, a mutation in an ABC transporter gene (ABCC2) is linked to Cry1Ac resistance in Plutella xylostella. Cry1AcMod, engineered to lack helix α-1, was able to form oligomers without cadherinbinding and effectively countered Cry1Ac resistance linked to ABCC2. Here we analyzed Cry1Ac and Cry1AcMod binding and oligomerization by western blots using brush border membrane vesicles (BBMV) from a strain of P. xylostella susceptible to Cry1Ac (Geneva 88) and a strain with resistance to Cry1Ac (NO-QAGE) linked to an ABCC2 mutation. Resistance correlated with lack of specific binding and reduced oligomerization of Cry1Ac in BBMV from NO-QAGE. In contrast, Cry1AcMod bound specifically and still formed oligomers in BBMV from both strains. We compared association of pre-formed Cry1Ac oligomer, obtained by incubating Cry1Ac toxin with a Manduca sexta cadherin fragment, with BBMV from both strains. Our results show that pre-formed oligomers associate more efficiently with BBMV from Geneva 88 than with BBMV from NO-QAGE, indicating that the ABCC2 mutation also affects the association of Cry1Ac oligomer with the membrane. These data indicate, for the first time, that ABCC2 facilitates Cry1Ac oligomerization and oligomer membrane insertion in P. xylostella.

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Role of alkaline phosphatase in insecticidal action of Cry1Ac against Helicoverpa armigera larvae

Cited by 28 publications

References 43 publications

Receptors of Garlic (Allium sativum) Lectins and Their Role in Insecticidal Action

Receptors of Garlic (Allium sativum) Lectins and Their Role in Insecticidal Action

Transgenic tomato line expressing modified Bacillus thuringiensis cry1Ab gene showing complete resistance to two lepidopteran pests

ABCC2 is associated with Bacillus thuringiensis Cry1Ac toxin oligomerization and membrane insertion in diamondback moth

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