Role of Actin Cytoskeleton in E-cadherin-Based Cell–Cell Adhesion Assembly and Maintenance

Rasool, Saad; Geethakumari, Anupriya M.; Biswas, Kabir H.

doi:10.1007/s41745-020-00214-0

Cited by 8 publications

(6 citation statements)

References 133 publications

(98 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Overall, nuclear β-catenin staining is strongly associated with poor prognosis in tumor patients [ 82 , 85 ]. The disruption of E-cadherin adhesion and its down-regulation, as we have shown after repeated NMP exposure, have essential roles in cell proliferation and are a common epithelial cancer feature [ 86 ]. Also, the mRNA level of glycogen synthase kinase (GSK) 3β and the nuclear lymphoid enhancer-biding factor (LEF) 1 were consistent with findings of previous studies.…”

Section: Discussionmentioning

confidence: 99%

“…One important role of the actin cytoskeleton is regulating the β-catenin / E-cadherin complex between adherent cells. The actin cytoskeleton maintains and changes the cellular shape and structural stability of cells and is involved in cell–cell and cell–matrix adhesion [ 86 ]. Individual components of the actin cytoskeleton (e.g., stress fibers, filipodia, lamellipodia, focal adhesions) define the mechanical properties of the cell surface [ 37 ], which were strongly changed upon NMP treatment.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Short- and long-term polystyrene nano- and microplastic exposure promotes oxidative stress and divergently affects skin cell architecture and Wnt/beta-catenin signaling

et al. 2023

View full text Add to dashboard Cite

Nano- and microplastic particles (NMP) are strong environmental contaminants affecting marine ecosystems and human health. The negligible use of biodegradable plastics and the lack of knowledge about plastic uptake, accumulation, and functional consequences led us to investigate the short- and long-term effects in freshly isolated skin cells from mice. Using fluorescent NMP of several sizes (200 nm to 6 µm), efficient cellular uptake was observed, causing, however, only minor acute toxicity as metabolic activity and apoptosis data suggested, albeit changes in intracellular reactive species and thiol levels were observed. The internalized NMP induced an altered expression of various targets of the nuclear factor-2-related transcription factor 2 pathway and were accompanied by changed antioxidant and oxidative stress signaling responses, as suggested by altered heme oxygenase 1 and glutathione peroxide 2 levels. A highly increased beta-catenin expression under acute but not chronic NMP exposure was concomitant with a strong translocation from membrane to the nucleus and subsequent transcription activation of Wnt signaling target genes after both single-dose and chronic long-term NMP exposure. Moreover, fibroblast-to-myofibroblast transdifferentiation accompanied by an increase of α smooth muscle actin and collagen expression was observed. Together with several NMP-induced changes in junctional and adherence protein expression, our study for the first time elucidates the acute and chronic effects of NMP of different sizes in primary skin cells' signaling and functional biology, contributing to a better understanding of nano- and microplastic to health risks in higher vertebrates.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Short- and long-term polystyrene nano- and microplastic exposure promotes oxidative stress and divergently affects skin cell architecture and Wnt/beta-catenin signaling

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Considering the impact on cell morphology of PE N/MPLs that we highlighted by TEM, we underlined a transient breakdown of the actin cytoskeleton in vaginal cells exposed to the highest concentration of PE particles. Indeed, the actin cytoskeleton is responsible for the structural stability of cells by maintaining and adapting the cellular shape, and mediating cell-cell and cell-matrix adhesion [53]. Further, we observed β-catenin accumulation at the cell junction upon low PE particle concentration exposure, while it moved towards the nucleus at high PE N/MPL concentrations.…”

Section: Discussionmentioning

confidence: 60%

Assessing the Impact of Polyethylene Nano/Microplastic Exposure on Human Vaginal Keratinocytes

Pontecorvi,

Ceccarelli,

Cece

et al. 2023

IJMS

View full text Add to dashboard Cite

The global rise of single-use throw-away plastic products has elicited a massive increase in the nano/microplastics (N/MPLs) exposure burden in humans. Recently, it has been demonstrated that disposable period products may release N/MPLs with usage, which represents a potential threat to women’s health which has not been scientifically addressed yet. By using polyethyl ene (PE) particles (200 nm to 9 μm), we showed that acute exposure to a high concentration of N/MPLs induced cell toxicity in vaginal keratinocytes after effective cellular uptake, as viability and apoptosis data suggest, along with transmission electron microscopy (TEM) observations. The internalised N/MPLs altered the expression of junctional and adherence proteins and the organisation of the actin cortex, influencing the level of genes involved in oxidative stress signalling pathways and that of miRNAs related to epithelial barrier function. When the exposure to PE N/MPLs was discontinued or became chronic, cells were able to recover from the negative effects on viability and differentiation/proliferation gene expression in a few days. However, in all cases, PE N/MPL exposure prompted a sustained alteration of DNA methyltransferase and DNA demethylase expression, which might impact epigenetic regulation processes, leading to accelerated cell ageing and inflammation, or the occurrence of malignant transformation.

show abstract

“…There are four negatively poled calcium ion binding sides between the five EC-domains each binding three Calcium ions. The EC1s of neighbouring cells interact calcium-dependently in a homophilic manner and mediate rigid cell–cell adhesion [ 36 , 37 ]. Cis interactions of the EC1 domain with the EC2 domain lead to further clustering [ 38 ].…”

Section: Molecular Genetics and Histopathological Alterations In Hdgcmentioning

confidence: 99%

Hereditary Diffuse Gastric Cancer: Molecular Genetics, Biological Mechanisms and Current Therapeutic Approaches

Cosma

Schlosser

Tews

et al. 2022

IJMS

View full text Add to dashboard Cite

Hereditary diffuse gastric cancer is an autosomal dominant syndrome characterized by a high prevalence of diffuse gastric cancer and lobular breast cancer. It is caused by inactivating mutations in the tumor suppressor gene CDH1. Genetic testing technologies have become more efficient over the years, also enabling the discovery of other susceptibility genes for gastric cancer, such as CTNNA1 among the most important genes. The diagnosis of pathogenic variant carriers with an increased risk of developing gastric cancer is a selection process involving a multidisciplinary team. To achieve optimal long-term results, it requires shared decision-making in risk management. In this review, we present a synopsis of the molecular changes and current therapeutic approaches in HDGC based on the current literature.

show abstract

Role of Actin Cytoskeleton in E-cadherin-Based Cell–Cell Adhesion Assembly and Maintenance

Cited by 8 publications

References 133 publications

Short- and long-term polystyrene nano- and microplastic exposure promotes oxidative stress and divergently affects skin cell architecture and Wnt/beta-catenin signaling

Short- and long-term polystyrene nano- and microplastic exposure promotes oxidative stress and divergently affects skin cell architecture and Wnt/beta-catenin signaling

Assessing the Impact of Polyethylene Nano/Microplastic Exposure on Human Vaginal Keratinocytes

Hereditary Diffuse Gastric Cancer: Molecular Genetics, Biological Mechanisms and Current Therapeutic Approaches

Contact Info

Product

Resources

About