Role of Actin Cytoskeleton During Mammalian Sperm Acrosomal Exocytosis

Romarowski, Ana; Luque, Guillermina M.; Spina, Florenza Antonella la; Krapf, Darío; Buffone, Mariano G.

doi:10.1007/978-3-319-30567-7_7

Cited by 20 publications

(14 citation statements)

References 99 publications

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“…Filamentous actin (F-actin) is a key factor in exocytosis in many cell types. In several mammalian species, AR is regulated by the actin cytoskeleton (Romarowski, Luque, et al, 2016). However, the dynamic changes of the actin cytoskeleton in live sperm could not be followed unless actin was revealed by expressing fluorescent proteins together with the actin monomers.…”

Section: Dynamic Changes Of the Actin Cytoskeleton During The Armentioning

confidence: 99%

Seeing is believing: Current methods to observe sperm acrosomal exocytosis in real time

Balestrini

Jabloñski

Schiavi‐Ehrenhaus

et al. 2020

Molecular Reproduction Devel

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Acrosomal exocytosis (AR) is a critical process that sperm need to undergo to fertilize an egg. The evaluation of the presence or absence of the acrosome is usually performed by using lectins or dyes in fixed cells. With this approach, it is neither possible to monitor the dynamic process of exocytosis and related molecular events while discriminating between live and dead cells, nor to evaluate the acrosomal status while sperm reside in the female reproductive tract. However, over the last two decades, several new methodologies have been used to assess the occurrence of AR in living cells allowing different groups to obtain information that was not possible in the past. These techniques have revolutionized the whole study of this process. This review summarizes current methods available to analyze AR in living cells as well as the important information that emerged from studies using these approaches.

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Section: Dynamic Changes Of the Actin Cytoskeleton During The Armentioning

confidence: 99%

Seeing is believing: Current methods to observe sperm acrosomal exocytosis in real time

Balestrini

Jabloñski

Schiavi‐Ehrenhaus

et al. 2020

Molecular Reproduction Devel

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“…The scaffolding framework that maintains the organization of the sperm flagellum cytoskeleton and its differentiated structures is poorly understood. In addition to the axoneme, actin and actinassociated proteins have been documented in sperm from many species (Romarowski et al, 2016). Actin is an abundant and highly conserved protein among eukaryotes, and is essential for diverse cellular functions, such as cell shape maintenance, motility, membrane organization (Krapf, 2018) and cytokinesis (Campellone and Welch, 2010).…”

Section: Introductionmentioning

confidence: 99%

The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure

Gervasi

Carbajal-González

et al. 2018

Journal of Cell Science

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Conception in mammals is determined by the fusion of a sperm cell with an oocyte during fertilization. Motility is one of the features of sperm that allows them to succeed in fertilization, and their flagellum is essential for this function. Longitudinally, the flagellum can be divided into the midpiece, the principal piece and the end piece. A precise cytoskeletal architecture of the sperm tail is key for the acquisition of fertilization competence. It has been proposed that the actin cytoskeleton plays essential roles in the regulation of sperm motility; however, the actin organization in sperm remains elusive. In the present work, we show that there are different types of actin structures in the sperm tail by using three-dimensional stochastic optical reconstruction microscopy (STORM). In the principal piece, actin is radially distributed between the axoneme and the plasma membrane. The actin-associated proteins spectrin and adducin are also found in these structures. Strikingly, polymerized actin in the midpiece forms a double-helix that accompanies mitochondria. Our findings illustrate a novel specialized structure of actin filaments in a mammalian cell.

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“…The signaling cascade of acrosomal exocytosis, induced by the binding of sperm head ligands to ZPC, activates signaling through phospholipases, and both the tyrosine and serine/threonine protein kinases which leads to the influx of external calcium, depolymerization of the actin microfilaments scaffolding the acrosome, and the activation of membrane fusion pathways leading to the fusion and hybridizing vesiculation of plasma membrane and OAM, resulting in the formation of acrosomal shroud (Belmonte et al, 2016; Romarowski et al, 2016).…”

Section: Sperm–zona Pellucida Interactionsmentioning

confidence: 99%

Review: Sperm–oocyte interactions and their implications for bull fertility, with emphasis on the ubiquitin–proteasome system

Šutovský

2018

Animal

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Fertilization is an intricate cascade of events that irreversibly alter the participating male and female gamete and ultimately lead to the union of paternal and maternal genomes in the zygote. Fertilization starts with sperm capacitation within the oviductal sperm reservoir, followed by gamete recognition, sperm-zona pellucida interactions and sperm-oolemma adhesion and fusion, followed by sperm incorporation, oocyte activation, pronuclear development and embryo cleavage. At fertilization, bull spermatozoon loses its acrosome and plasma membrane components and contributes chromosomes, centriole, perinuclear theca proteins and regulatory RNAs to the zygote. While also incorporated in oocyte cytoplasm, structures of the sperm tail, including mitochondrial sheath, axoneme, fibrous sheath and outer dense fibers are degraded and recycled. The ability of some of these sperm contributed components to give rise to functional zygotic structures and properly induce embryonic development may vary between bulls, bearing on their reproductive performance, and on the fitness, health, fertility and production traits of their offspring. Proper functioning, recycling and remodeling of gamete structures at fertilization is aided by the ubiquitin-proteasome system (UPS), the universal substrate-specific protein recycling pathway present in bovine and other mammalian oocytes and spermatozoa. This review is focused on the aspects of UPS relevant to bovine fertilization and bull fertility.

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Role of Actin Cytoskeleton During Mammalian Sperm Acrosomal Exocytosis

Cited by 20 publications

References 99 publications

Seeing is believing: Current methods to observe sperm acrosomal exocytosis in real time

Seeing is believing: Current methods to observe sperm acrosomal exocytosis in real time

The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure

Review: Sperm–oocyte interactions and their implications for bull fertility, with emphasis on the ubiquitin–proteasome system

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