Role of AcndtA in cleistothecium formation, osmotic stress response, pigmentation and carbon metabolism of Aspergillus cristatus

Wang, Yaping; Tan, Yi; Wang, Yuchen; Liu, Yongxiang; Liu, Hui; Shao, Lei; Liu, Yimei; Ren, Xiuxiu; Zuo-yi, Liu

doi:10.1016/j.funbio.2021.04.009

Cited by 7 publications

(12 citation statements)

References 72 publications

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“…Pigment production is commonly carried out as a response to environmental stressors such as toxic metals, UV or ionizing radiation [ 41 , 42 ]. The genus Aspergillus is known to produce a variety of pigments, including several types of melanin, naphthopyrones, parasperone A or aspergillin, which are often present in vegetative structures such as spores, hyphae and sclerotia [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex

Harpke

Pietschmann

Ueberschaar

et al. 2022

Genes

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Three strains of the Aspergillus versicolor complex were isolated from a salty marsh at a former uranium mining site in Thuringia, Germany. The strains from a metal-rich environment were not only highly salt tolerant (up to 20% NaCl), but at the same time could sustain elevated Cs and Sr (both up to 100 mM) concentrations as well as other (heavy) metals present in the environment. During growth experiments when screening for differential cell morphology, the occurrence of guttation droplets was observed, specifically when elevated Sr concentrations of 25 mM were present in the media. To analyze the potential of metal tolerance being promoted by these excretions, proteomics and metabolomics of guttation droplets were performed. Indeed, proteins involved in up-regulated metabolic activities as well as in stress responses were identified. The metabolome verified the presence of amino sugars, glucose homeostasis-regulating substances, abscisic acid and bioactive alkaloids, flavones and quinones.

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Section: Discussionmentioning

confidence: 99%

Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex

Harpke

Pietschmann

Ueberschaar

et al. 2022

Genes

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“…A standard curve was established using the genomic DNA of ΔAcndtA. Southern blot analysis verified that the foreign gene in the ΔAcndtA strain was inserted in a single copy [7].…”

Section: Nucleic Acid Isolation and Rt-qpcr Analysismentioning

confidence: 99%

“…Deletion of veA resulted in reduced sexual sporulation and increased conidium production [6]. Mutation of the AcndtA gene caused the development of the cleistothecium to stagnate at an early stage [7]. The sporulation mechanism of A. cristatus remains unclear.…”

Section: Introductionmentioning

confidence: 99%

“…Although several zinc finger transcription factors have been reported to play an important role in fungal development, the molecular mechanisms of fungal reproduction have not been elucidated. The p53-like transcription factor AcndtA was found to regulate fungal reproduction, pigmentation, and stress response in A. cristatus [7]. A total of 25 development-related genes were significantly regulated in an AcndtA mutant compared with the wild-type (WT) [7].…”

Section: Introductionmentioning

confidence: 99%

“…The p53-like transcription factor AcndtA was found to regulate fungal reproduction, pigmentation, and stress response in A. cristatus [7]. A total of 25 development-related genes were significantly regulated in an AcndtA mutant compared with the wild-type (WT) [7]. Five of them were zinc finger transcription factor genes.…”

Section: Introductionmentioning

confidence: 99%

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Characterizing the role of the zinc finger transcription factor AcrpnR in governing development in Aspergillus cristatus

et al. 2021

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Filamentous fungi reproduce sexually or asexually, and the developmental processes are strictly regulated by a variety of transcription factors. In this study, we characterized a zinc finger transcription factor, called AcrpnR, in Aspergillus cristatus (GME2916). The ΔAcrpnR strain exhibited decreased asexual reproduction and increased cleistothecium production. The complementation strain showed restoration of these phenotypic differences. Overexpression of AcrpnR resulted in enhanced asexual development and delayed and inhibited sexual reproduction, suggesting that AcrpnR is required for proper asexual and sexual development in A.cristatus. In addition, AcrpnR positively regulated the expression of genes of the central regulatory pathway of conidiation and negatively regulated the expression of sex-related genes. Overall, these results demonstrate that AcrpnR is essential for maintaining a balance between asexual and sexual development.

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The role of Acpbs2 in the asexual sporulation, stress response and carbon metabolism of Aspergillus cristatus

et al. 2022

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Aspergillus cristatus is the dominant fungus during the fermentation of Fuzhuan brick tea, hypotonic conditions only induced its sexual development to produce ascospores, while hypertonic conditions only induced its asexual development to produce conidia, indicating that osmotic stress can regulate spore production in A. cristatus. However, the underlying regulatory mechanism is unclear. In this study, the roles of Acpbs2, which is homologous to pbs2 from Saccharomyces cerevisiae, in sporulation, stress responses, the color of colonies, and carbon metabolism were explored in A. cristatus.Deletion mutants of Acpbs2 were obtained by homologous recombination. The time required to produce conidia was delayed, and the number of conidia produced was significantly reduced in hypertonic media in ΔAcpbs2 by phenotypic observations, indicating that Acpbs2 plays a positive role in asexual development. Stress sensitivity tests showed that the order of the sensitivity of ΔAcpbs2 to different osmotic regulators was 3 M NaCl > 3 M sucrose > 3 M sorbitol. Moreover, the deletion mutants were sensitive to high oxidative stress. The growth of the Acpbs2 deletion mutant was inhibited under alkaline-pH stress, indicating that Acpbs2 is involved in high pH stress tolerance. Additionally, compared with the wild type, the colony color of the Acpbs2 deletion mutant became lighter. All the above developmental defects were reversed by the reintroduction of the Acpbs2 gene in ΔAcpbs2. Transcriptome data showed that Acpbs2 regulated the expression of several genes related to conidial development, osmotic stress, oxidative stress, and carbon metabolism. More importantly, the interaction between Acpbs2 and its downstream gene Achog1 was verified by yeast two-hybrid assays. We speculated that this interaction might regulate the osmotic stress response, the oxidative stress response, and asexual sporulation in A. cristatus, which will be one of the focuses of our future research.

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Role of AcndtA in cleistothecium formation, osmotic stress response, pigmentation and carbon metabolism of Aspergillus cristatus

Cited by 7 publications

References 72 publications

Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex

Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex

Characterizing the role of the zinc finger transcription factor AcrpnR in governing development in Aspergillus cristatus

The role of Acpbs2 in the asexual sporulation, stress response and carbon metabolism of Aspergillus cristatus

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