Role of ACE and AGT gene polymorphisms in genetic susceptibility to diabetes mellitus type 2 in a Brazilian sample

Abstract

ABSTRACT. The aim of the current study was to investigate the association between the InDel polymorphism in the angiotensin I-converting enzyme gene (ACE) and the rs699 polymorphism in the angiotensinogen gene (AGT) and diabetes mellitus type 2 (DM2) in a sample population from Southern Brazil. A case-control study was conducted with 228 patients with DM2 and 183 controls without DM2. The ACE InDel polymorphism was genotyped by polymerase chain reaction (PCR) with specific primers, followed by electrophoresis on 1.5% agarose gel. The AGT rs699 polymorphism was genotyped using a real-time PCR assay. No significant 19111 ACE and AGT gene polymorphisms in diabetes mellitus type 2 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 19110-19116 (2015) association between the ACE InDel polymorphism and DM2 was detected (P = 0.97). However, regarding the AGT rs699 polymorphism, DM2 patients had a significantly higher frequency of the AG genotype and lower frequency of the GG genotype when compared to the controls (P = 0.03). Our results suggest that there is an association between the AGT rs699 polymorphism and DM2 in a Brazilian sample.

Association of angiotensin-converting enzyme insertion/deletion (ACE I/D) and angiotensinogen (AGT M235T) polymorphisms with the risk of obesity in a Tunisian population

“…A number of important research papers have extensively searched the link of ACE I/D and AGT M235T with several diseases, mainly hypertension 22,10 and diabetes, [23][24][25] often with highly inconsistent findings throughout various ethnic populations worldwide; associations with overweight and obesity, an important risk factor for cardiovascular diseases, have also been reported. 9,26,27 In this context, ACE I/D and AGT M235T polymorphisms association studies in the Tunisian population are very limited, with reports linking them to obesity.…”

Lack of association between ACE ID genetic polymorphism and diabetes or hypertension in Brazilians aged from 50 to 70 years old

“…Baroudi et al, 21 studying the association between the ACE ID genotype and type 2 diabetes in older Jerbian individuals, demonstrated a higher DD genotype frequency in the diabetes group than the control group. On the other hand, Wollinger et al, 22 researching the older Brazilian population, demonstrated a lack of association between the ACE genotypes and type 2 diabetes.…”

Effect of the cream based on Croton lechleri Müll. Arg. in the treatment of diabetic patients' ulcers: a case study

“…There is still low number of prescriptions and use of phytotherapics in Vale do Taquari, region where Lajeado city is located, including for the DM control (REMPEL et al, 2010), even after a sequence of analyzes carried out by the research group with diabetic individuals at Universidade do Vale do Taquari in Lajeado (BENINI et al, 2009);BENINI et al, 2010;HELLER et al, 2013;MARMITT et al, 2015;MIRALLES;MORAES et al, 2010;NAVA et al, 2015;POZZOBON et al, 2014;POZZOBON et al, 2012;REMPEL et al, 2015;REMPEL et al, 2010;RODRIGUES et al, 2010;VANZETTO et al, 2011;WOLLINGER et al, 2015;ZACCARON et al, 2014). It should be emphasized that studies indicate healing potential for the sap of C. lechleri (CAY et al, 1991;PHILLIPSON, 1993;PHILLIPSON, 1994;PIETERS et al, 1992;SILVA et al, 2019).…”