Role of A-type ARABIDOPSIS RESPONSE REGULATORS in meristem maintenance and regeneration

Buechel, Sabine; Leibfried, Andrea; To, Jennifer P.C.; Zhao, Zhong; Andersen, Stig Uggerhøj; Kieber, Joseph J.; Lohmann, Jan U.

doi:10.1016/j.ejcb.2009.11.016

Cited by 112 publications

(103 citation statements)

References 39 publications

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“…STM expression levels were upregulated 2.1-fold in 7-day-old msl2 msl3 mutants, but were not distinguishable from the wild type at 21 DAG. Consistent with previous observations that they inhibit callus formation (Buechel et al, 2010;Liu et al, 2016), ARR7 and ARR15 transcript levels were reduced in both 7-and 21-day-old msl2 msl3 mutant seedlings compared with the wild type, exhibiting 43-55% and 13-44% decreases, respectively. Transcriptional repression is specific to ARR7 and ARR15, as transcript levels of two other Atype ARR genes, ARR4 and ARR5, were elevated in msl2 msl3 mutant seedlings compared with the wild type.…”

Section: Msl2 Msl3 Double Mutants Develop Callus At the Shoot Apexsupporting

confidence: 77%

Plastid osmotic stress influences cell differentiation at the plant shoot apex

Wilson¹,

Mixdorf²,

Berg³

et al. 2016

Journal of Cell Science

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The balance between proliferation and differentiation in the plant shoot apical meristem is controlled by regulatory loops involving the phytohormone cytokinin and stem cell identity genes. Concurrently, cellular differentiation in the developing shoot is coordinated with the environmental and developmental status of plastids within those cells. Here, we employ an Arabidopsis thaliana mutant exhibiting constitutive plastid osmotic stress to investigate the molecular and genetic pathways connecting plastid osmotic stress with cell differentiation at the shoot apex. msl2 msl3 mutants exhibit dramatically enlarged and deformed plastids in the shoot apical meristem, and develop a mass of callus tissue at the shoot apex. Callus production in this mutant requires the cytokinin receptor AHK2 and is characterized by increased cytokinin levels, downregulation of cytokinin signaling inhibitors ARR7 and ARR15, and induction of the stem cell identity gene WUSCHEL. Furthermore, plastid stressinduced apical callus production requires elevated plastidic reactive oxygen species, ABA biosynthesis, the retrograde signaling protein GUN1, and ABI4. These results are consistent with a model wherein the cytokinin/WUS pathway and retrograde signaling control cell differentiation at the shoot apex.

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Section: Msl2 Msl3 Double Mutants Develop Callus At the Shoot Apexsupporting

confidence: 77%

Plastid osmotic stress influences cell differentiation at the plant shoot apex

Wilson¹,

Mixdorf²,

Berg³

et al. 2016

Journal of Cell Science

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“…However, several family members need to be repressed to achieve noticeable cytokinin hypersensitivity or enhanced callus induction and shoot regeneration in culture. 17,27 This genetic redundancy is a major limitation to using Type-A response regulators to improve regeneration from tissue culture.…”

Section: Manipulating the Cytokinin Response To Enhance Regeneration mentioning

confidence: 99%

Enhancing plant regeneration in tissue culture

Hill

Schäller

2013

Plant Signaling & Behavior

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Micropropagation is used for commercial purposes worldwide, but the capacity to undergo somatic organogenesis and plant regeneration varies greatly among species. The plant hormones auxin and cytokinin are critical for plant regeneration in tissue culture, with cytokinin playing an instrumental role in shoot organogenesis. Type-B response regulators govern the transcriptional output in response to cytokinin and are required for plant regeneration. In our paper published in Plant Physiology, we explored the functional redundancy among the 11 type-B Arabidopsis response regulators (ARRs). Interestingly, we discovered that the enhanced expression of one family member, ARR10, induced hypersensitivity to cytokinin in multiple assays, including callus greening and shoot induction of explants. Here we 1) discuss the hormone dependence for in vitro plant regeneration, 2) how manipulation of the cytokinin response has been used to enhance plant regeneration, and 3) the potential of the ARR10 transgene as a tool to increase the regeneration capacity of agriculturally important crop plants. The efficacy of ARR10 for enhancing plant regeneration likely arises from its ability to transcriptionally regulate key cytokinin responsive genes combined with an enhanced protein stability of ARR10 compared with other type-B ARRs. By increasing the capacity of key tissues and cell types to respond to cytokinin, ARR10, or other type-B response regulators with similar properties, could be used as a tool to combat

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“…An Arabidopsis shoot induction system described previously was adopted for the analysis of shoot regeneration in this study (Buechel et al, 2010). First, root explants were pretreated in an auxin-rich callus induction medium (CIM) to generate callus.…”

Section: Arr1 Arr10 and Arr12 Display Dynamic Expression Patterns Dmentioning

confidence: 99%

“…Mutations in type-B ARABIDOPSIS RESPONSE REGULATORs (ARRs), key regulators of primary cytokinin response genes, result in reduced shoot regeneration (Ishida et al, 2008;Hwang et al, 2012;Hill et al, 2013;Zhang et al, 2015). By contrast, overexpressing type-A ARRs encoding negative regulators of cytokinin signaling suppress shoot formation (Buechel et al, 2010). Moreover, spatial biosynthesis, polar transport, and signaling transduction of auxin are required for shoot regeneration (Gordon et al, 2007;Kareem et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Type-B ARABIDOPSIS RESPONSE REGULATORs Specify the Shoot Stem Cell Niche by Dual Regulation of WUSCHEL

et al. 2017

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ORCID IDs: 0000-0003-3103-8629 (W.J.M.); 0000-0002-3129-5206 (X.S.Z.)Plants are known for their capacity to regenerate the whole body through de novo formation of apical meristems from a mass of proliferating cells named callus. Exogenous cytokinin and auxin determine cell fate for the establishment of the stem cell niche, which is the vital step of shoot regeneration, but the underlying mechanisms remain unclear. Here, we show that type-B ARABIDOPSIS RESPONSE REGULATORs (ARRs), critical components of cytokinin signaling, activate the transcription of WUSCHEL (WUS), which encodes a key regulator for maintaining stem cells. In parallel, type-B ARRs inhibit auxin accumulation by repressing the expression of YUCCAs, which encode a key enzyme for auxin biosynthesis, indirectly promoting WUS induction. Both pathways are essential for de novo regeneration of the shoot stem cell niche. In addition, the dual regulation of type-B ARRs on WUS transcription is required for the maintenance of the shoot apical meristem in planta. Thus, our results reveal a long-standing missing link between cytokinin signaling and WUS regulator, and the findings provide critical information for understanding cell fate specification.

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Role of A-type ARABIDOPSIS RESPONSE REGULATORS in meristem maintenance and regeneration

Cited by 112 publications

References 39 publications

Plastid osmotic stress influences cell differentiation at the plant shoot apex

Plastid osmotic stress influences cell differentiation at the plant shoot apex

Enhancing plant regeneration in tissue culture

Type-B ARABIDOPSIS RESPONSE REGULATORs Specify the Shoot Stem Cell Niche by Dual Regulation of WUSCHEL

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