Role of 5-HT3 Receptor on Food Intake in Fed and Fasted Mice

Li, Bingjin; Shao, Dongyuan; Luo, Yungang; Wang, Pu; Liu, Changhong; Zhang, Xingyi; Cui, Ranji

doi:10.1371/journal.pone.0121473

Cited by 15 publications

(14 citation statements)

References 47 publications

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“…Moreover, (i) there was a significant difference in diarrhoea severity score between intervened and mock-treated mice at 48 and 72 h p.i, (ii) mock-treated mice had significantly more total diarrhoea output than intervened mice and (iii) Ondansetron-treatment resulted in better weight gain ( Fig 8A–8E ). The latter finding should be put in the light of observations that serotonin help control food intake [ 78 , 79 ] and that feeding behaviour in fasting mice may be related to C-fos activity in the hypothalamus and brainstem [ 80 ]. It should also be noted that RV infection activates C-fos in CNS [ 3 ], which suggests a common signalling pathway that may result in less appetite during RV illness.…”

Section: Discussionmentioning

confidence: 99%

Rotavirus and Serotonin Cross-Talk in Diarrhoea

et al. 2016

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Rotavirus (RV) has been shown to infect and stimulate secretion of serotonin from human enterochromaffin (EC) cells and to infect EC cells in the small intestine of mice. It remains to identify which intracellularly expressed viral protein(s) is responsible for this novel property and to further establish the clinical role of serotonin in RV infection. First, we found that siRNA specifically silencing NSP4 (siRNANSP4) significantly attenuated secretion of serotonin from Rhesus rotavirus (RRV) infected EC tumor cells compared to siRNAVP4, siRNAVP6 and siRNAVP7. Second, intracellular calcium mobilization and diarrhoeal capacity from virulent and avirulent porcine viruses correlated with the capacity to release serotonin from EC tumor cells. Third, following administration of serotonin, all (10/10) infants, but no (0/8) adult mice, responded with diarrhoea. Finally, blocking of serotonin receptors using Ondansetron significantly attenuated murine RV (strain EDIM) diarrhoea in infant mice (2.9 vs 4.5 days). Ondansetron-treated mice (n = 11) had significantly (p < 0.05) less diarrhoea, lower diarrhoea severity score and lower total diarrhoea output as compared to mock-treated mice (n = 9). Similarly, Ondansetron-treated mice had better weight gain than mock-treated animals (p < 0.05). A most surprising finding was that the serotonin receptor antagonist significantly (p < 0.05) also attenuated total viral shedding. In summary, we show that intracellularly expressed NSP4 stimulates release of serotonin from human EC tumor cells and that serotonin participates in RV diarrhoea, which can be attenuated by Ondansetron.

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Section: Discussionmentioning

confidence: 99%

Rotavirus and Serotonin Cross-Talk in Diarrhoea

et al. 2016

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“…Mazzola-Pomietto and colleagues [52] reported a transient inhibition of feeding (within the first hour), followed by transient hyperphagia (between 1 and 4 hr), after systemic mCPBG treatment (10 mg/kg) in food-restricted male Wistar rats, though chin-rubbing behavior may have interfered with food intake early in the session. Recently, a similar transient inhibition of acute feeding was observed following SR 57227 (a 5-HT 3 agonist) treatment in food restricted, but not fed, mice [60]. That 5-HT 3 receptor stimulation does not appear to consistently reduce feeding when given systemically may suggest differential involvement of 5-HT 3 receptors in food intake under different dietary and deprivation conditions across the hindbrain (see introduction), the ventral tegmentum, and the NAcc.…”

Section: 0 Discussionmentioning

confidence: 77%

Contrasting effects of 5-HT3 receptor stimulation of the nucleus accumbens or ventral tegmentum on food intake in the rat

Pratt

Lin

Pierce-Messick

et al. 2017

Behavioural Brain Research

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Although serotonin (5-HT) signaling is known to regulate food intake and energy homeostasis, the roles of the 5-HT3 receptor in feeding processes has been elusive. 5-HT3 receptors are found throughout mesolimbic circuitry that promote feeding not only in response to hunger, but also to the palatable and rewarding properties of food. These experiments examined if stimulation or blockade of the 5-HT3 receptor of the nucleus accumbens (NAcc) or ventral tegmentum affected food intake in the rat in response to hunger or the presence of a palatable diet. Rats (N = 6–9/group) received bilateral injections of the 5-HT3 agonist m-chlorophenylbiguanide hydrochloride (mCPBG; at 0.0, 10.0, or 20.0 µg/0.5µl/side) or the 5-HT3 antagonist ondansetron hydrochloride (at 0.0, 1.0, 2.0, or 5.0 µg/0.5µl/side) into either the NAcc or the ventral tegmentum. NAcc 5-HT3 receptor stimulation significantly increased 2-hr food intake in food-deprived animals offered rat chow and in a separate group of unrestricted rats offered a sweetened fat diet. In contrast to the feeding increase seen with NAcc treatments, stimulation of 5-HT3 receptors of the ventral tegmentum significantly reduced food and water intake in food-restricted animals; reductions of intake in non-restricted rats offered the palatable diet did not approach significance. Blockade of the 5-HT3 receptor had no effect on feeding in either brain region. These data support a functional role for serotonergic signaling in the mesolimbic pathway on motivated behavior, and demonstrate that 5-HT3 receptors differentially modulate food consumption in a region-dependent manner.

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“…We also observed that pretreatment with baclofen reversed leptin‐induced reduction of food intake in fasted mice, indicating that GABA‐B receptor might be implicated in the regulation of food intake by leptin. We have previously reported that 5‐HT 3 receptor agonist SR‐57227 significantly inhibited the food intake of fasted mice which can be blocked by 5‐HT 3 receptor antagonist ondansetron . Some evidence have shown that systematic baclofen administration can increase the food intake of animals in a dose‐dependent manner .…”

Section: Discussionmentioning

confidence: 99%

Role of leptin in the regulation of food intake in fasted mice

Yao

Zhao

et al. 2020

J Cellular Molecular Medi

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Leptin is well acknowledged as an anorexigenic hormone that plays an important role in feeding control. Hypothalamic GABA system plays a significant role in leptin regulation on feeding and metabolism control. However, the pharmacological relationship of leptin and GABA receptor is still obscure. Therefore, we investigated the effect of leptin or combined with baclofen on the food intake in fasted mice. We detected the changes in hypothalamic c-Fos expression, hypothalamic TH, POMC and GAD67 expression, plasma insulin, POMC and GABA levels to demonstrate the mechanisms.We found that leptin inhibit fasting-induced increased food intake and activated hypothalamic neurons. The inhibitory effect on food intake induced by leptin in fasted mice can be reversed by pretreatment with baclofen. Baclofen reversed leptin's inhibition on c-Fos expression of PAMM in fasted mice. Therefore, these results indicate that leptin might inhibit fasting-triggered activation of PVN neurons via presynaptic GABA synaptic functions which might be partially blocked by pharmacological activating GABA-B. Our findings identify the role of leptin in the regulation of food intake. K E Y W O R D S baclofen, feeding behaviour, GABA-B, hypothalamus, leptin | 4525 GE Et al.

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Role of 5-HT3 Receptor on Food Intake in Fed and Fasted Mice

Cited by 15 publications

References 47 publications

Rotavirus and Serotonin Cross-Talk in Diarrhoea

Rotavirus and Serotonin Cross-Talk in Diarrhoea

Contrasting effects of 5-HT3 receptor stimulation of the nucleus accumbens or ventral tegmentum on food intake in the rat

Role of leptin in the regulation of food intake in fasted mice

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