Role for Periodontitis in the Progression of Lipid Deposition in an Animal Model

Jain, Ashish; Batista, Evander de Jesus Oliveira; Serhan, Charles N.; Stahl, Gregory L.; Dyke, Thomas E. Van

doi:10.1128/iai.71.10.6012-6018.2003

Cited by 150 publications

(135 citation statements)

References 22 publications

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“…Colonies were then selected and anaerobically subcultured at 37°C for 48 hr in brain heart infusion broth supplemented with hemin and menadione. P. gingivalis turbidity (OD 0.8) was spectrophotometrically determined at 600 nm, and bacteria were mixed with 4% sodium carboxymethyl cellulose (Wako Pure Chemical Industries, Tokyo; Jain, Batista, Serhan, Stahl, & Van Dyke, 2003). …”

Section: Methodsmentioning

confidence: 99%

Porphyromonas gingivalis induced periodontitis exacerbates progression of non‐alcoholic steatohepatitis in rats

Kuraji

Fujita

et al. 2016

Clinical & Exp Dental Res

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Non‐alcoholic steatohepatitis (NASH) is a chronic liver disease that can develop into hepatocirrhosis and hepatic carcinoma. In recent years, epidemiological and animal studies have reported that Porphyromonas gingivalis (P. gingivalis), a known periodontopathic bacteria, is closely related to NASH. However, previous studies could not demonstrate a direct relationship between periodontitis, P. gingivalis infection, and NASH. The purpose of the present study was to examine the impact of P. gingivalis‐associated periodontitis on the onset and progression of NASH. Forty‐two male Wistar rats were used in this study. Rats were fed a high‐fat diet (HFD) for 12 weeks in order to induce fatty liver. At 4 weeks from the start of feeding, the animals were performed ligature placement around the maxillary first molar tooth in order to induce experimental periodontitis, and then a P. gingivalis slurry was applied around the ligature twice in a week for 8 weeks (HFD/Pg(+) group). Controls were given the slurry without P. gingivalis after ligature placement using the same protocol (HFD/Pg(−) group). Significant increases in alveolar bone resorption and inflammation in periodontal tissue around the molar tooth in the HFD/Pg(+) group were observed when compared with the HFD/Pg(−) group. Moreover, histological images showing NASH characterized by perivenular lipid deposition including big fatty drops, ballooning degeneration, and focal necrosis with inflammatory cells were confirmed in the liver of rats in the HFD/Pg(+) group. Significant increases in alanine aminotransaminase, aspartate aminotransferase, and C‐reactive protein levels were observed in the HFD/Pg(+) group. Furthermore, endotoxin levels in serum in the HFD/Pg(+) group were significantly higher than those in the HFD/Pg(−) group. The present study demonstrated that experimental periodontitis induced by P. gingivalis led to the progression of NASH in rats with fatty liver. Increased levels of endotoxin derived from P. gingivalis infection appear to play a considerable role in the progression of NASH.

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Section: Methodsmentioning

confidence: 99%

Porphyromonas gingivalis induced periodontitis exacerbates progression of non‐alcoholic steatohepatitis in rats

Kuraji

Fujita

et al. 2016

Clinical & Exp Dental Res

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“…Periodontal pathogens, such as Aggregatibacter actinomycetemcomitans, Treponema denticola and Porphyromonas gingivalis are also capable of invading endothelial cells [23–27], and they have been detected in atherosclerotic plaques, heart valves, aortic aneurysms, carotid and coronary vessels [28–33]. Studies in a variety of animal models have demonstrated that recurrent bacteraemia or oral administration with P. gingivalis can enhance atherogenesis [34,35]. Of interest, P. gingivalis is so far the only bacterium capable of causing enzymatic citrullination of peptides with subsequent development of anti-citrullinated peptide auto-antibodies, a major etiopathologic event in RA [36].…”

Section: Introductionmentioning

confidence: 99%

Defining the gut microbiota in individuals with periodontal diseases: an exploratory study

Lourenςo

Spencer

Alm

et al. 2018

Journal of Oral Microbiology

109

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Background: This exploratory study aimed to characterize the gut microbiome of individuals with different periodontal conditions, and correlate it with periodontal inflammation and tissue destruction.Methods: Stool samples were obtained from individuals presenting periodontal health (PH = 7), gingivitis (G = 14) and chronic periodontitis (CP = 23). The intestinal microbiome composition was determined by Illumina MiSeq sequencing.Results: A lower alpha-diversity in the gut microbiome of individuals with CP was observed, although no significant difference among groups was found (p > 0.01). Firmicutes, Proteobacteria, Verrucomicrobia and Euryarchaeota were increased, whereas Bacteroidetes were decreased in abundance in patients with periodontitis compared to PH. Prevotella (genus), Comamonadaceae (family) and Lactobacillales (order) were detected in higher numbers in G, while Bacteroidales (order) was predominant in PH (p < 0.01). Significant correlations (rho = 0.337–0.468, p < 0.01) were found between OTUs representative of periodontal pathogens and attachment loss. Mogibacteriaceae, Ruminococcaceae and Prevotella were able to discriminate individuals with periodontal diseases from PH (overall accuracy = 84%). Oral taxa were detected in high numbers in all stool samples.Conclusions: Individuals with periodontal diseases present a less diverse gut microbiome consistent with other systemic inflammatory diseases. High numbers of oral taxa related to periodontal destruction and inflammation were detected in the gut microbiome of individuals regardless of periodontal status.

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“…7 One potential pathway linking tooth loss and vascular disease risk is oral infection-inflammation related to periodontal disease. 6 -18 Periodontal disease, a local chronic bacterial infection in the oral cavity, may contribute to endothelial dysfunction, 12 carotid artery plaque formation, 11,13,15,17 or deterioration of the antiatherogenic potency of HDL. 18 A second pathway is that tooth loss may affect dietary quality and nutrient intake, leading to an increased risk of vascular diseases.…”

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confidence: 99%

Tooth Loss Is Associated With an Increased Risk of Hypertension in Postmenopausal Women

et al. 2004

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Abstract-Tooth loss has been associated with an increased risk of vascular diseases such as coronary heart disease and cerebrovascular disease. Little is known whether hypertension is an important factor linking 2 phenomena in postmenopausal women. We compared an incidence of hypertension and traditional risk factors for vascular diseases between 2 age-matched groups: 67 postmenopausal women with missing teeth and 31 without missing teeth. In addition to blood pressure, serum concentration of total cholesterol, high-and low-density lipoprotein cholesterol and triglycerides, plasma angiotensin-converting enzyme activity, plasma angiotensin II concentration, plasma renin activity, and resting heart rate were measured as traditional risk factors for vascular diseases. Subjects without missing teeth had significantly lower diastolic blood pressure than did subjects with missing teeth (Pϭ0.021). The former tended to have lower systolic blood pressure than did the latter (Pϭ0.058). There were no significant differences in other variables between subjects with and without missing teeth. The odds ratio of having hypertension in subjects with missing teeth was 3.59 (95% confidence interval, 1.10 to 11.7) after adjustment of obesity, hypercholesterolemia, and hypertriglyceridemia. Our results suggest that hypertension may be an important factor linking tooth loss and an increased risk of vascular diseases in postmenopausal women.

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Role for Periodontitis in the Progression of Lipid Deposition in an Animal Model

Cited by 150 publications

References 22 publications

Porphyromonas gingivalis induced periodontitis exacerbates progression of non‐alcoholic steatohepatitis in rats

Porphyromonas gingivalis induced periodontitis exacerbates progression of non‐alcoholic steatohepatitis in rats

Defining the gut microbiota in individuals with periodontal diseases: an exploratory study

Tooth Loss Is Associated With an Increased Risk of Hypertension in Postmenopausal Women

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