Role for nitrate assimilatory genes in virulence of Ustilago maydis

Khanal, Sunita; Schroeder, Luke A.; Nava-Mercado, Otniel Alejandro; Mendoza, Hector; Perlin, Michael H.

doi:10.1016/j.funbio.2021.04.010

Cited by 2 publications

(3 citation statements)

References 63 publications

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“…To address this possibility, we analyzed the proposed nitrate permease from U . maydis (UMAG_11105, nrt1 from here, see S11 Fig for characterization of this permease) [ 54 , 55 ]. In control cells, a GFP-tagged allele of Nrt1 was located at PM.…”

Section: Resultsmentioning

confidence: 99%

“…This inhibition would result in nitrogen starvation conditions, despite the presence of nitrate in the growth medium. To address this possibility, we analyzed the proposed nitrate permease from U. maydis (UMAG_11105, nrt1 from here, see S11 Fig for characterization of this permease) [54,55]. In control cells, a GFP-tagged allele of Nrt1 was located at PM.…”

Section: Overactivation Of Torc1 Affects the Ability Of Cells To Grow...mentioning

confidence: 99%

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The Rheb GTPase promotes pheromone blindness via a TORC1-independent pathway in the phytopathogenic fungus Ustilago maydis

Torre

Perez-Martin

2022

PLoS Genet

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The target of the rapamycin (TOR) signaling pathway plays a negative role in controlling virulence in phytopathogenic fungi. However, the actual targets involved in virulence are currently unknown. Using the corn smut fungus Ustilago maydis, we tried to address the effects of the ectopic activation of TOR on virulence. We obtained gain-of-function mutations in the Rheb GTPase, one of the conserved TOR kinase regulators. We have found that unscheduled activation of Rheb resulted in the alteration of the proper localization of the pheromone receptor, Pra1, and thereby pheromone insensitivity. Since pheromone signaling triggers virulence in Ustilaginales, we believe that the Rheb-induced pheromone blindness was responsible for the associated lack of virulence. Strikingly, although these effects required the concourse of the Rsp5 ubiquitin ligase and the Art3 α-arrestin, the TOR kinase was not involved. Several eukaryotic organisms have shown that Rheb transmits environmental information through TOR-dependent and -independent pathways. Therefore, our results expand the range of signaling manners at which environmental conditions could impinge on the virulence of phytopathogenic fungi.

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Section: Resultsmentioning

confidence: 99%

Section: Overactivation Of Torc1 Affects the Ability Of Cells To Grow...mentioning

confidence: 99%

The Rheb GTPase promotes pheromone blindness via a TORC1-independent pathway in the phytopathogenic fungus Ustilago maydis

Torre

Perez-Martin

2022

PLoS Genet

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“…U. maydis can use nitrate as the sole nitrogen source. Khanal et al ( 2021 ) characterized the role of nitrate assimilation genes in the virulence of U. maydis , including two genes, um03848 and um03849 , which serve as nitrite reductase and nitrate transporter, respectively. The deletion mutants for um03848, um03849 , or both genes were obtained by homologous recombination.…”

Section: Application Progress Of Crispr/cas9 In Edible Fungimentioning

confidence: 99%

Application progress of CRISPR/Cas9 genome-editing technology in edible fungi

et al. 2023

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Edible fungi are not only delicious but are also rich in nutritional and medicinal value, which is highly sought after by consumers. As the edible fungi industry continues to rapidly advance worldwide, particularly in China, the cultivation of superior and innovative edible fungi strains has become increasingly pivotal. Nevertheless, conventional breeding techniques for edible fungi can be arduous and time-consuming. CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9) is a powerful tool for molecular breeding due to its ability to mediate high-efficiency and high-precision genome modification, which has been successfully applied to many kinds of edible fungi. In this review, we briefly summarized the working mechanism of the CRISPR/Cas9 system and highlighted the application progress of CRISPR/Cas9-mediated genome-editing technology in edible fungi, including Agaricus bisporus, Ganoderma lucidum, Flammulina filiformis, Ustilago maydis, Pleurotus eryngii, Pleurotus ostreatus, Coprinopsis cinerea, Schizophyllum commune, Cordyceps militaris, and Shiraia bambusicola. Additionally, we discussed the limitations and challenges encountered using CRISPR/Cas9 technology in edible fungi and provided potential solutions. Finally, the applications of CRISPR/Cas9 system for molecular breeding of edible fungi in the future are explored.

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Role for nitrate assimilatory genes in virulence of Ustilago maydis

Cited by 2 publications

References 63 publications

The Rheb GTPase promotes pheromone blindness via a TORC1-independent pathway in the phytopathogenic fungus Ustilago maydis

The Rheb GTPase promotes pheromone blindness via a TORC1-independent pathway in the phytopathogenic fungus Ustilago maydis

Application progress of CRISPR/Cas9 genome-editing technology in edible fungi

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