Role and mechanism of CD90+ fibroblasts in inflammatory diseases and malignant tumors

Abstract

Fibroblasts are highly heterogeneous mesenchymal stromal cells, and different fibroblast subpopulations play different roles. A subpopulation of fibroblasts expressing CD90, a 25–37 kDa glycosylphosphatidylinositol anchored protein, plays a dominant role in the fibrotic and pro-inflammatory state. In this review, we focused on CD90+ fibroblasts, and their roles and possible mechanisms in disease processes. First, the main biological functions of CD90+ fibroblasts in inducing angiogenesis and maintaining tissue homeostasis are described. Second, the role and possible mechanism of CD90+ fibroblasts in inducing pulmonary fibrosis, inflammatory arthritis, inflammatory skin diseases, and scar formation are introduced, and we discuss how CD90+ cancer-associated fibroblasts might serve as promising cancer biomarkers. Finally, we propose future research directions related to CD90+ fibroblasts. This review will provide a theoretical basis for the diagnosis and treatment CD90+ fibroblast-related disease.

Identification of a set of genes potentially responsible for resistance to ferroptosis in lung adenocarcinoma cancer stem cells

“…For the immunophenotypic characterization of the primary cultures, the expression levels of some specific markers were analysed by flow cytometry. In particular: epithelial cell adhesion molecule (EpCAM), expressed in epithelial cells and correlated with tumorigenesis [ 17 ]; Cytokeratin 7 (CK7), normally produced by lung epithelial cells and commonly used as a marker for diagnosis of lung tumors [ 18 ]; CD90 (alias Thymus cell antigen 1), a well-known marker of fibroblast [ 19 , 20 ]; CD45, a ubiquitous cell surface marker of nucleated hematopoietic cells [ 21 ]. The LUAD primary cell cultures isolated exhibited a marked positivity for EpCAM and CK7/8 markers, assuring that they were constituted by cancer cells of epithelial origin with the same phenotypic features of the original tumor, as well as negligible expression of CD90 and CD45 markers, thus excluding the presence of fibroblasts and tumor-infiltrating immune cells (Table 1 ).…”

The mechanisms and therapeutic potential of clopidogrel in mitigating diabetic cardiomyopathy in db/db mice

“…CD90 is a surface marker specific for fibroblasts. 21 , 22 Based on this, two-color immunofluorescence was used to identify the transition of cardiac fibroblasts to myofibroblasts in mouse cardiac tissue. We observed a significant increase in this transition in DM mice, which was markedly reduced after treatment with Clo ( Figure S1 B).…”

Multi‑dimensional analysis reveals NCKAP5L is a promising biomarker for the diagnosis and prognosis of human cancers, especially colorectal cancer

“…As an important component of the tumor microenvironment, fibroblasts are important mesenchymal stem cells that constitute tissues and organs of organisms and may serve roles in biological processes such as tissue fibrosis and collagen secretion (49)(50)(51). Cancer-related fibroblasts have been extensively studied and are associated with the occurrence and development of cancers (52)(53)(54)(55).…”