Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina

Rivero, Mariana; Passucci, Juan; Rodríguez, Edgardo; Parma, A

doi:10.1099/jmm.0.015560-0

Cited by 59 publications

(48 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This observation is in line with findings in neighboring countries, such as The Netherlands (60), Germany (2), France (51), Spain (5), and Switzerland (26,27). In contrast to the situation in these continental European countries, the proportions of O157 and non-O157 infections are thought to be quite different in North America, Argentina, and the United Kingdom (53,55). Yet a recent study by Hedican et al showed that non-O157 infections may account for as many as 53% of human VTEC isolates in the United States (19).…”

Section: Discussionsupporting

confidence: 86%

Incidence and Virulence Determinants of Verocytotoxin-Producing Escherichia coli Infections in the Brussels-Capital Region, Belgium, in 2008–2010

et al. 2012

View full text Add to dashboard Cite

hThe incidence of verocytotoxin-producing Escherichia coli (VTEC) was investigated by PCR in all human stools from Universitair Ziekenhuis Brussel (UZB) and in selected stools from six other hospital laboratories in the Brussels-Capital Region, Belgium, collected between April 2008 and October 2010. The stools selected to be included in this study were those from patients with hemolytic-uremic syndrome (HUS), patients with a history of bloody diarrhea, patients linked to clusters of diarrhea, children up to the age of 6 years, and stools containing macroscopic blood. Verocytotoxin genes (vtx) were detected significantly more frequently in stools from patients with the selected conditions (2.04%) than in unselected stools from UZB (1.20%) (P ‫؍‬ 0.001). VTEC was detected most frequently in patients with HUS (35.3%), a history of bloody diarrhea (5.15%), or stools containing macroscopic blood (1.85%). Stools from patients up to the age of 17 years were significantly more frequently vtx positive than those from adult patients between the ages of 18 and 65 years (P ‫؍‬ 0.022). Although stools from patients older than 65 years were also more frequently positive for vtx than those from patients between 18 and 65 years, this trend was not significant. VTEC was isolated from 140 (67.9%) vtx-positive stools. One sample yielded two different serotypes; thus, 141 isolates could be characterized. Sixty different O:H serotypes harboring 85 different virulence profiles were identified. Serotypes O157:H7/H؊ (n ‫؍‬ 34), O26:H11/H؊ (n ‫؍‬ 21), O63:H6 (n ‫؍‬ 8), O111:H8/H؊ (n ‫؍‬ 7), and O146:H21/H؊ (n ‫؍‬ 6) accounted for 53.9% of isolates. All O157 isolates carried vtx2, eae, and a complete O island 122 (COI-122); 15 also carried vtx1. Non-O157 isolates (n ‫؍‬ 107), however, accounted for the bulk (75.9%) of isolates. Fifty-nine (55.1%) isolates were positive for vtx1, 36 (33.6%) were positive for vtx2, and 12 (11.2%) carried both vtx1 and vtx2. Pulsed-field gel electrophoresis revealed wide genetic diversity; however, small clusters of O157, O26, and O63:H6 VTEC that could have been part of unidentified outbreaks were identified. Antimicrobial resistance was observed in 63 (44.7%) isolates, and 34 (24.1%) showed multidrug resistance. Our data show that VTEC infections were not limited to patients with HUS or bloody diarrhea. Clinical laboratories should, therefore, screen all stools for O157 and non-O157 VTEC using selective media and a method for detecting verocytotoxins or vtx genes. V erocytotoxin-producing Escherichia coli (VTEC), also called Shiga toxin-producing E. coli (STEC), is associated with diarrhea, often bloody, that may be complicated with hemorrhagic colitis and the life-threatening hemolytic-uremic syndrome (HUS), especially in children and the elderly (29). VTEC is characterized by its ability to produce one or more phage-encoded verocytotoxins, VT1 and VT2, that show distinct immunogenic and genetic properties (42). Multiple subtypes of VT1 (VT1a, VT1c, and VT1d) and VT2 (VT2a to VT2g), with significant diff...

show abstract

Section: Discussionsupporting

confidence: 86%

Incidence and Virulence Determinants of Verocytotoxin-Producing Escherichia coli Infections in the Brussels-Capital Region, Belgium, in 2008–2010

et al. 2012

View full text Add to dashboard Cite

show abstract

“…In the present study the isolation rate of E. coli serotype O157:H7 was 28.9 %, which is lower than the isolation rate documented by Rivas et al 28 and Rivero et al 29 . These differences could be due to sampling of children with different types of acute diarrhea, rather than the study being oriented towards bloody diarrhea or to children in contact with hemolytic uremic syndrome (HUS) patients.…”

Section: Discussioncontrasting

confidence: 53%

Antibiogram of E. coli serotypes isolated from children aged under five with acute diarrhea in Bahir Dar town

et al. 2015

View full text Add to dashboard Cite

Background: Diarrheal disease and its complications remain a major cause of morbidity and mortality in children. The prevalence and antibiogram of E. coli as causative agents of diarrhea vary from region to region, and even within countries in the same geographical area. Objectives: To determine the serotype and antimicrobial susceptibility of E. coli in children under-five years of age. Methods: A cross-sectional study was conducted among 422 children with diarrhea from December 2011 to February 2012. Identification of E. coli and antimicrobial susceptibility testing were done following standard procedures. Results: The overall isolation rate of E. coli was 48.3%. Poly 2 sero-groups, poly 3 sero-groups, poly 4 sero-groups and E. coli O157:H7 accounted for 80 (39.2%), 40 (19.6%), 25 (12.3%), and 59 (28.9%) of the isolates, respectively. Poly 2 sero-groups, constituting isolates belonging to enteropathogenic E. coli were the most commonly isolated serotypes. E. coli exhibited high levels of antimicrobial resistance to ampicillin (86.8%), tetracycline (76%) and cotrimoxazole (76%). Low levels of resistance to ciprofloxacin (6.9%) and norfloxacin (9.3%) were documented. Conclusion: High prevalence of diarrheagenic E. coli compounded by alarming antimicrobial resistances is a serious public health problem. Regular determination of antibiogram and public education are recommended.

show abstract

“…It should be noted that the ECDC/EFSA recommends not comparing incidences between countries due to differences in detection. STEC is also prevalent in developing countries such as Argentina, which has been described in the literature to have the highest worldwide incidence of HUS in children under the age of 5 (197). This may be due to excessive exposure to known risk factors associated with STEC infections, including meat consumption, playing in recreational water, and poor personal hygiene (198).…”

Section: Epidemiologymentioning

confidence: 99%

Recent Advances in Understanding Enteric Pathogenic Escherichia coli

et al. 2013

View full text Add to dashboard Cite

SUMMARY Although Escherichia coli can be an innocuous resident of the gastrointestinal tract, it also has the pathogenic capacity to cause significant diarrheal and extraintestinal diseases. Pathogenic variants of E. coli (pathovars or pathotypes) cause much morbidity and mortality worldwide. Consequently, pathogenic E. coli is widely studied in humans, animals, food, and the environment. While there are many common features that these pathotypes employ to colonize the intestinal mucosa and cause disease, the course, onset, and complications vary significantly. Outbreaks are common in developed and developing countries, and they sometimes have fatal consequences. Many of these pathotypes are a major public health concern as they have low infectious doses and are transmitted through ubiquitous mediums, including food and water. The seriousness of pathogenic E. coli is exemplified by dedicated national and international surveillance programs that monitor and track outbreaks; unfortunately, this surveillance is often lacking in developing countries. While not all pathotypes carry the same public health profile, they all carry an enormous potential to cause disease and continue to present challenges to human health. This comprehensive review highlights recent advances in our understanding of the intestinal pathotypes of E. coli .

show abstract

Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina

Cited by 59 publications

References 37 publications

Incidence and Virulence Determinants of Verocytotoxin-Producing Escherichia coli Infections in the Brussels-Capital Region, Belgium, in 2008–2010

Incidence and Virulence Determinants of Verocytotoxin-Producing Escherichia coli Infections in the Brussels-Capital Region, Belgium, in 2008–2010

Antibiogram of E. coli serotypes isolated from children aged under five with acute diarrhea in Bahir Dar town

Recent Advances in Understanding Enteric Pathogenic Escherichia coli

Contact Info

Product

Resources

About