Rohu Fish (Labio Rohita) Bile Attenuates Streptozotocin- Induced Diabetes Mellitus in Rats: Evidence from Biochemical and Oxidative Stress Markers

Abstract

Aim: Present study was undertaken to validate folklore claims regarding antidiabetic activity of the bile of 'Rohu' (Labeo rohita) fish.Method: Diabetes was induced by single injection of Streptozotocin (50 mg/ kg) in Wistar rats. Diabetic rats were screened and treated with bile (0.08 and 0.16 ml/kg, p.o) daily for 14 days. Fasting blood glucose level was estimated at specific intervals. Serum lipid profiles, serum biochemical parameters related to diabetic pathophysiology and hepatic redox makers were estimated.Result: Oral administration of bile to diabetic rats caused significant and dose dependant hypoglycaemic activity with a maximum reduction of blood glucose level (~77 %, p < 0.001) on day 15 at the dose of 0.16ml/kg. Bile treatment also could significantly (p < 0.001) control hyperlipidemia and restore serum biochemical parameters near to normal values. Diabetic control rats showed a significant (p < 0.001) rise in lipid peroxidation and depletion (p < 0.001) of antioxidant enzymes in hepatic tissue of diabetic rats as compared with normal rats. Bile treatment, however, could significantly (p < 0.05-0.01) revert these changes near to the normal status. Conclusion:The Labeo rohita fish bile is capable of managing hyperglycaemia, hyperlipidemia and associated complications in diabetes.

Exploring fish in a new way: A review on non-food industrial applications of fish

“…However, physicians did not report any significant difference in function of insulin derived from fish and domestic animals [ 38 ]. Labeo rohita bile attenuates caused significant hypoglycemic activity in streptozotocin-induced diabetes mellitus in rats [ 39 ]. Amount of insulin found in some fishes has been presented in Table 1 [ 38 ].…”