ROESY and 13C NMR to distinguish between <scp>d</scp>- and <scp>l</scp>-rhamnose in the α-<scp>d</scp>-Manp-(1 → 4)-β-Rhap-(1 → 3) repeating motif

Davidson, Jeffrey; Gauthier-Signore, Charles; Bishop, Kevin P.; Wicks, Christopher; Monteiro, Mário A.; Roy, Pierre‐Nicholas; Auzanneau, France‐Isabelle

doi:10.1039/d2ob00131d

Cited by 5 publications

(2 citation statements)

References 48 publications

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“…Another group we noticed is the genera Enterocloster which had a stepped growth of abundance in groups of health, IBS without-and with psychiatric disorders. Enterocloster has been reported to increase in individuals suffering from GI symptoms and autism [29]. Interestingly, we observed that Enterocloster was also signi cantly enriched in IBS-D patients compared with IBS-C and healthy controls.…”

Section: Discussionsupporting

confidence: 46%

An Integrated Multicenter Amplicon Sequencing Data Reveals New Evidence of the Interaction Between the Gut Microbiota and Irritable Bowel Syndrome

Chen

Tang

et al. 2022

Preprint

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Background Gut dysbacteriosis has been reported as one of the etiologies for irritable bowel syndrome (IBS). However, the association between gut microbiota and IBS is still inconclusive. The study aims to provide new evidence of the interaction between the gut Microbiota and IBS. Method A paired-sample study was designed by retrieving original multicenter 16s-rRNA data of IBS patients and healthy controls from the GMrepo database. The propensity score matching (PSM) algorithm was applied to reduce confounding bias. The differential analysis of microbiota composition was performed at different taxonomic levels. The co-occurrence network was established. Subgroup analysis was performed to identify specific microbial compositions in different IBS subtypes. Results A total of 1522 amplicon samples were initially enrolled. After PSM, 708 samples (354 IBS and 354 healthy individuals) were eligible for further analysis. A total of 1,160 genera were identified. We identified significantly changed taxa in IBS groups (IBS-enriched: the families Enterobacteriaceae, Moraxellaceae, and Sphingobacteriaceae; the genera Streptococcus, Bacillus, Enterocloster, Sphingobacterium, Holdemania, and Acinetobacter. IBS-depleted: the phyla Firmicutes, Euryarchaeota, Cyanobacteria, Acidobacteria, and Lentisphaerae; the families Bifidobacteriaceae, Ruminococcaceae, Methanobacteriaceae, and the other 25 families; the genera Faecalibacterium, Bifidobacterium, and other 68 genera). In subgroup analysis, we profiled microbial compositions in IBS with predominant diarrhea and constipation. We further identified the genera Bilophila and Enterocloster that may be involved in linking IBS with psychiatric disorders. The co-occurrence network identified three hub genera and six hub species (including Faecalibacterium prausnitzii) that may be important in IBS pathophysiology. Strong positive interactions were identified among the Bifidobacterium longum, Bifidobacterium breve, and Bifidobacterium adolescentis in the Bifidobacterium community. Conclusion This study provides updated evidence in identifying specific microbes that may involve in IBS pathogenesis. Future modalities may be further validated by targeting these microorganisms.

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Section: Discussionsupporting

confidence: 46%

An Integrated Multicenter Amplicon Sequencing Data Reveals New Evidence of the Interaction Between the Gut Microbiota and Irritable Bowel Syndrome

Chen

Tang

et al. 2022

Preprint

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“…In some cases, when the absolute configuration of a specific monosaccharide residue of a glycan is known, the absolute configuration of the directly attached monosaccharides can be established through the analysis of 13 C NMR glycosylation shifts. , Interestingly, this strategy has proven useful to assign the absolute configuration of Rha residues linked to a structurally conserved N-glycan moiety of the major capsid protein of some chloroviruses. Depending on the virus species, the rhamnose residue that is linked to O3 of a trisubstituted l -Fuc p residue can have the d- or l -configuration; as predicted, a larger glycosylation shift (displacement) is observed for C1 of the d -Rha residue when compared to that of l -Rha .…”

Section: Identification Of Structural Partsmentioning

confidence: 99%

Primary Structure of Glycans by NMR Spectroscopy

Fontana

Widmalm

2023

Chem. Rev.

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Glycans, carbohydrate molecules in the realm of biology, are present as biomedically important glycoconjugates and a characteristic aspect is that their structures in many instances are branched. In determining the primary structure of a glycan, the sugar components including the absolute configuration and ring form, anomeric configuration, linkage(s), sequence, and substituents should be elucidated. Solution state NMR spectroscopy offers a unique opportunity to resolve all these aspects at atomic resolution. During the last two decades, advancement of both NMR experiments and spectrometer hardware have made it possible to unravel carbohydrate structure more efficiently. These developments applicable to glycans include, inter alia, NMR experiments that reduce spectral overlap, use selective excitations, record tilted projections of multidimensional spectra, acquire spectra by multiple receivers, utilize polarization by fast-pulsing techniques, concatenate pulse-sequence modules to acquire several spectra in a single measurement, acquire pure shift correlated spectra devoid of scalar couplings, employ stable isotope labeling to efficiently obtain homo-and/or heteronuclear correlations, as well as those that rely on dipolar cross-correlated interactions for sequential information. Refined computer programs for NMR spin simulation and chemical shift prediction aid the structural elucidation of glycans, which are notorious for their limited spectral dispersion. Hardware developments include cryogenically cold probes and dynamic nuclear polarization techniques, both resulting in enhanced sensitivity as well as ultrahigh field NMR spectrometers with a 1 H NMR resonance frequency higher than 1 GHz, thus improving resolution of resonances. Taken together, the developments have made and will in the future make it possible to elucidate carbohydrate structure in great detail, thereby forming the basis for understanding of how glycans interact with other molecules.

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Structure and synthesis of a vaccine and diagnostic target for Enterocloster bolteae, an autism-associated gut pathogen – Part II

Frame

Allas

Pequegnat

et al. 2023

Carbohydrate Research

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ROESY and ¹³C NMR to distinguish between d- and l-rhamnose in the α-d-Manp-(1 → 4)-β-Rhap-(1 → 3) repeating motif

Abstract: Many children suffering from autism spectrum disorder (ASD) experience gastrointestinal (GI) conditions. Enterocloster bolteae has been regularly detected in the stool of individuals suffering from GI symptoms and autism. Literature...

Cited by 5 publications

References 48 publications

An Integrated Multicenter Amplicon Sequencing Data Reveals New Evidence of the Interaction Between the Gut Microbiota and Irritable Bowel Syndrome

An Integrated Multicenter Amplicon Sequencing Data Reveals New Evidence of the Interaction Between the Gut Microbiota and Irritable Bowel Syndrome

Primary Structure of Glycans by NMR Spectroscopy

Structure and synthesis of a vaccine and diagnostic target for Enterocloster bolteae, an autism-associated gut pathogen – Part II

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ROESY and 13C NMR to distinguish between d- and l-rhamnose in the α-d-Manp-(1 → 4)-β-Rhap-(1 → 3) repeating motif

Abstract: Many children suffering from autism spectrum disorder (ASD) experience gastrointestinal (GI) conditions. Enterocloster bolteae has been regularly detected in the stool of individuals suffering from GI symptoms and autism. Literature...

Cited by 5 publications

References 48 publications

An Integrated Multicenter Amplicon Sequencing Data Reveals New Evidence of the Interaction Between the Gut Microbiota and Irritable Bowel Syndrome

An Integrated Multicenter Amplicon Sequencing Data Reveals New Evidence of the Interaction Between the Gut Microbiota and Irritable Bowel Syndrome

Primary Structure of Glycans by NMR Spectroscopy

Structure and synthesis of a vaccine and diagnostic target for Enterocloster bolteae, an autism-associated gut pathogen – Part II

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ROESY and ¹³C NMR to distinguish between d- and l-rhamnose in the α-d-Manp-(1 → 4)-β-Rhap-(1 → 3) repeating motif