Rodent Models of Methamphetamine Misuse: Mechanisms of Methamphetamine Action and Comparison of Different Rodent Paradigms

Hasan, Hiba; Abdelhady, Samar; Haidar, Muhammad; Fakih, Christina; Hayek, Samer El; Mondello, Stefania; Kobeissy, Firas; Shaito, Abdullah

doi:10.1007/978-1-4939-9554-7_13

Cited by 2 publications

(2 citation statements)

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“…Further characterization of these differences is crucial to evaluate the impact of the drug on human health and to characterize its various interactions with other drugs that can exacerbate neurotoxicity. One common theme among these drugs is their neurotoxic effects on the brain leading to what is observed in brain injury [43,[57][58][59][60][61].…”

Section: Discussionmentioning

confidence: 99%

Effects of Cathinones (Bath Salts) On Cultured Primary Neurons/Astroglia Cells and Neurobehavioral Functions in Mice

Selig¹,

Pierre²,

Arja³

et al. 2022

Preprint

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This study aims to examine the cytotoxicity mechanisms of synthetic cathinone (bath salts) on rat primary cultured neurons and primary astroglial cells, and to assess their neurobehavioral effects on mice. We administered methylenedioxypyrovalerone (MDPV) to both rat primary cultured neurons and primary astroglial cells to assess cell injury. We also analyzed the effects of MDPV on these cell cultures using immunocytochemistry. We utilized western blotting to assess the breakdown of αII-spectrin and glial fibrillary acidic protein (GFAP) induced by MDPV. The western blotting experiment also included calpain and caspase inhibitors (SNJ1945 and Z-D-DCB, respectively) and pro-apoptotic and pro-necrotic agents (Staurosporine and calcium ionophore A23187, respectively). Lastly, we assessed MDPV’s effects on behavioral effects using rotarod, locomotor activity, elevated plus maze, Morris water maze, forced swimming, and open field tests. MDPV caused a dose-dependent release of LDH in both cerebrocortical neuron-astroglia mixed cultures and primary astroglial cultures. MDPV also caused neurite breakages and astroglial process retraction on immunocytochemistry. Lastly, MDPV induced αII-spectrin breakdown in western blotting experiments. Co-administration of calpain and caspase inhibitors reduced the degradation of αII-spectrin and GFAP. MDPV administration also increased anxiety-like behavior and locomotor activity in the mice. Synthetic cathinones, which share structural similarities with methamphetamine, also induce significant neurotoxic effects and neurobehavioral effects on rodent models. These neurotoxic effects are likely mediated by calpain and caspase-induced apoptosis and necrosis, while astroglial death is likely only due to calpain activation. Therefore, further research may focus on pharmacological interventions targeting these pathways to mitigate the cytotoxic impact of cathinones in humans.

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Section: Discussionmentioning

confidence: 99%

Effects of Cathinones (Bath Salts) On Cultured Primary Neurons/Astroglia Cells and Neurobehavioral Functions in Mice

Selig¹,

Pierre²,

Arja³

et al. 2022

Preprint

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show abstract

“…Methamphetamine (MA), a new drug commonly known as “ice,” affects the activities of the peripheral nervous system and central nervous system ( 1 ). MA can lead to strong psychological dependence, neurotoxicity, and a high relapse rate and long-term abuse of MA can seriously damage the physical and psychological health of people ( 2 ).…”

Section: Introductionmentioning

confidence: 99%

The Association of High-Frequency Nut Intake With a Low Risk of Psychological Problems in Female Methamphetamine Users

et al. 2022

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BackgroundRecent years have witnessed a gradual increase in the number of female methamphetamine users. Meanwhile, female methamphetamine users are more likely to have psychological problems than male methamphetamine users. The association between diet and psychological problems have been found among non-methamphetamine user. The present study aims to investigate the relationship between dietary intake frequency and psychological problems in female methamphetamine users.Materials and MethodsA total of 109 female methamphetamine users, collected from a Compulsory Isolated Drug Rehabilitation Centre in northern China, participated in the study. All participants completed the Symptom Checklist 90 (SCL-90) questionnaire to assess psychological status. The relation of dietary intake frequency with the SCL-90 score was tested in partial correlation analysis. Multivariable regression models were used to calculate odds ratios to evaluate the association of dietary intake frequency with psychological problems.ResultsOf the current female methamphetamine population, 33 participants were diagnosed with psychological problems using SCL-90. In the terms of dietary intake frequency, the frequency of nut intake in the psychiatric symptom group was significantly lower than that in the asymptomatic group. However, there was no difference in the frequency of other food intakes between the two groups. The frequency of nut intake was negatively correlated with the total score of SCL-90 and 8 different symptom clusters of psychopathologies on SCL-90. Logistic regression analysis indicated that the increased frequency of nut intake was associated with a lower risk of psychological problems.ConclusionIn the female methamphetamine population, increasing the frequency of nut intake may reduce the risk of psychological problems for female methamphetamine users.

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Rodent Models of Methamphetamine Misuse: Mechanisms of Methamphetamine Action and Comparison of Different Rodent Paradigms

Cited by 2 publications

References 125 publications

Effects of Cathinones (Bath Salts) On Cultured Primary Neurons/Astroglia Cells and Neurobehavioral Functions in Mice

Effects of Cathinones (Bath Salts) On Cultured Primary Neurons/Astroglia Cells and Neurobehavioral Functions in Mice

The Association of High-Frequency Nut Intake With a Low Risk of Psychological Problems in Female Methamphetamine Users

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