Rodent Behavioral Testing to Assess Functional Deficits Caused by Microelectrode Implantation in the Rat Motor Cortex

Goss-Varley, Monika; Shoffstall, Andrew J.; Dona, Keith R.; McMahon, Justin A.; Lindner, Sydney C.; Ereifej, Evon S.; Capadona, Jeffrey R.

doi:10.3791/57829-v

Cited by 6 publications

(8 citation statements)

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“…Unfortunately, a varying degree of success was observed through these approaches [7,[26][27][28][29][30][31]. Some of the most successful approaches to mitigate the neuroinflammatory response indicate a dominant role of reactive microglia cells and infiltrating macrophages, as well as the stability of the local BBB [8,[32][33][34][35][36]. For example, the anti-inflammatory/antibiotic Minocycline has been shown to increase the longevity and quality of functional neural recordings [7].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of the cluster of differentiation 14 innate immunity pathway with IAXO-101 improves chronic microelectrode performance

et al. 2018

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Section: Introductionmentioning

confidence: 99%

Inhibition of the cluster of differentiation 14 innate immunity pathway with IAXO-101 improves chronic microelectrode performance

et al. 2018

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“…Stereotactic implantation can exert consequences on animal behavioral patterns also reflecting putative detrimental effects on well‐being . The comparison between electrode‐implanted animals and naive control animals at various time points did not reveal relevant behavioral or biochemical alterations.…”

Section: Discussionmentioning

confidence: 88%

“…Stereotactic implantation can exert consequences on animal behavioral patterns also reflecting putative detrimental effects on well-being. 27,28 The comparison between electrode-implanted animals and naive control animals at various time points did not reveal relevant behavioral or biochemical alterations. These data are in line with our previous findings, in which animals with depth-electrode implantation exhibited only very minor behavioral alterations 9 weeks after surgery.…”

Section: Discussionmentioning

confidence: 90%

Toward evidence‐based severity assessment in rat models with repeated seizures: II. Chemical post–status epilepticus model

et al. 2019

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Objective Considering the complexity of neuronal circuits and their epilepsy‐associated alterations, epilepsy models cannot be completely replaced by in vitro experimental approaches. Decisions about ethical approval of in vivo studies require a thorough weighing of the animal's burden and the benefit regarding the expected gain in knowledge. Methods Based on combined behavioral, biochemical, and physiological analyses, we assessed the impact on animal well‐being and condition in different phases of the pilocarpine post–status epilepticus (SE) model in rats. Results As a consequence of SE, increased levels of impairment were evident in the early postinsult phase and late chronic phase, whereas only mild impairment was observed in the interim phase. Parameters that stood out as sensitive indicators of animal distress include burrowing, which proved to be affected throughout all experimental phases, saccharin preference, fecal corticosterone metabolites, heart rate, and heart rate variability. Significance The cumulative burden with temporary but not long‐lasting phases of more pronounced impairment suggests a classification of severe as a basis for laboratory‐specific prospective and retrospective evaluation. Among the parameters analyzed, burrowing behavior and saccharin preference stand out as candidate parameters that seem to be well suited to obtain information about animal distress in epileptogenesis models.

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“…Another important consideration that needs to be further validated is the effect of tissue aspiration and GRIN lens implantation on the mechanisms underlying the effects of neuromodulation therapies [79]. For example, this procedure disrupts tissue within the motor cortex, striatum, and other structures which may be critical to the therapeutic effects of DBS.…”

Section: Validation Of the Pathological Model And Dbs Therapy In The ...mentioning

confidence: 99%

Calcium imaging in freely moving mice during electrical stimulation of deep brain structures

et al. 2021

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After decades of study in humans and animal models, there remains a lack of consensus regarding how the action of electrical stimulation on neuronal and non-neuronal elementse.g. neuropil, cell bodies, glial cells, etc.leads to the therapeutic effects of neuromodulation therapies. To further our understanding of neuromodulation therapies, there is a critical need for novel methodological approaches using state-of-the-art neuroscience tools to study neuromodulation therapy in preclinical models of disease. In this manuscript we outline one such approach combining chronic behaving single-photon microendoscope recordings in a pathological mouse model with electrical stimulation of a common deep brain stimulation (DBS) target. We describe in detail the steps necessary to realize this approach, as well as discuss key considerations for extending this experimental paradigm to other DBS targets for different therapeutic indications. Additionally, we make recommendations from our experience on implementing and validating the required combination of procedures that includes: the induction of a pathological model (6-OHDA model of Parkinson's disease) through an injection procedure, the injection of the viral vector to induce GCaMP expression, the implantation of the GRIN lens and stimulation electrode, and the installation of a baseplate for mounting the microendoscope. We proactively identify unique data analysis confounds occurring due to the combination of electrical stimulation and optical recordings and outline an approach to address these confounds. In order to validate the technical feasibility of this unique combination of experimental methods, we present data to demonstrate that 1) despite the complex multifaceted surgical procedures, chronic optical recordings of hundreds of cells combined with stimulation is achievable over week long periods 2) this approach enables measurement of differences in DBS evoked neural activity between anesthetized and awake conditions and 3) this combination of techniques can be used to measure electrical stimulation induced changes in neural activity during behavior in a pathological mouse model. These findings are presented to underscore the feasibility and potential utility of minimally constrained optical recordings to elucidate the mechanisms of DBS therapies in animal models of disease.

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Rodent Behavioral Testing to Assess Functional Deficits Caused by Microelectrode Implantation in the Rat Motor Cortex

Cited by 6 publications

References 0 publications

Inhibition of the cluster of differentiation 14 innate immunity pathway with IAXO-101 improves chronic microelectrode performance

Inhibition of the cluster of differentiation 14 innate immunity pathway with IAXO-101 improves chronic microelectrode performance

Toward evidence‐based severity assessment in rat models with repeated seizures: II. Chemical post–status epilepticus model

Calcium imaging in freely moving mice during electrical stimulation of deep brain structures

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