RocS drives chromosome segregation and nucleoid protection in Streptococcus pneumoniae

Chryslène, Mercy; Ducret, Adrien; Slager, Jelle; Lavergne, Jean‐Pierre; Freton, Céline; Nagarajan, Sathya Narayanan; Garcia, Pierre; Noirot‐Gros, Marie‐Françoise; Dubarry, Nelly; Nourikyan, Julien; Veening, Jan‐Willem; Grangeasse, Christophe

doi:10.1038/s41564-019-0472-z

Cited by 28 publications

(43 citation statements)

References 48 publications

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“…In S. pneumoniae, ParB interacts with DivIVA during cell division. It has also been shown that ParB participates in specific crosstalk between DNA segregation, cell division, and capsule formation in this organism [168,170]. S. pneumoniae's incomplete Par(A)B-parS system consists of four parS sites close to oriC, as well as an orphan parB gene [197].…”

Section: Parb's Role In Capsule Formation In S Pneumoniaementioning

confidence: 99%

“…The deletion of parB impairs chromosome segregation but only in a minor way and has no influence on bacterial growth [71]. Recent reports have shed light on the role of S. pneumoniae ParB, showing that it cooperates with two proteins, CpsD and RocS [168,170]. Capsular polysaccharide protein D (CpsD), a ParA-like ATPase, is a component of a protein complex involved in the synthesis and export of capsular polysaccharide.…”

Section: Parb's Role In Capsule Formation In S Pneumoniaementioning

confidence: 99%

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Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes

et al. 2020

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The segregation of newly replicated chromosomes in bacterial cells is a highly coordinated spatiotemporal process. In the majority of bacterial species, a tripartite ParAB-parS system, composed of an ATPase (ParA), a DNA-binding protein (ParB), and its target(s) parS sequence(s), facilitates the initial steps of chromosome partitioning. ParB nucleates around parS(s) located in the vicinity of newly replicated oriCs to form large nucleoprotein complexes, which are subsequently relocated by ParA to distal cellular compartments. In this review, we describe the role of ParB in various processes within bacterial cells, pointing out interspecies differences. We outline recent progress in understanding the ParB nucleoprotein complex formation and its role in DNA segregation, including ori positioning and anchoring, DNA condensation, and loading of the structural maintenance of chromosome (SMC) proteins. The auxiliary roles of ParBs in the control of chromosome replication initiation and cell division, as well as the regulation of gene expression, are discussed. Moreover, we catalog ParB interacting proteins. Overall, this work highlights how different bacterial species adapt the DNA partitioning ParAB-parS system to meet their specific requirements.

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Section: Parb's Role In Capsule Formation In S Pneumoniaementioning

confidence: 99%

Section: Parb's Role In Capsule Formation In S Pneumoniaementioning

confidence: 99%

Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes

et al. 2020

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“…Recent studies identified another ParB interacting partner in S. pneumoniae-the RocS protein. RocS is required for chromosome segregation but also interacts with FtsZ and CspD (Mercy et al, 2019). The above examples show that chromosome segregation and cell division are coupled due to the interactions of segregation proteins.…”

Section: Interactions Between Segregation and Cell Division Proteinsmentioning

confidence: 91%

Chromosome Segregation Proteins as Coordinators of Cell Cycle in Response to Environmental Conditions

Pióro

Jakimowicz

2020

Front. Microbiol.

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Chromosome segregation is a crucial stage of the cell cycle. In general, proteins involved in this process are DNA-binding proteins, and in most bacteria, ParA and ParB are the main players; however, some bacteria manage this process by employing other proteins, such as condensins. The dynamic interaction between ParA and ParB drives movement and exerts positioning of the chromosomal origin of replication (oriC) within the cell. In addition, both ParA and ParB were shown to interact with the other proteins, including those involved in cell division or cell elongation. The significance of these interactions for the progression of the cell cycle is currently under investigation. Remarkably, DNA binding by ParA and ParB as well as their interactions with protein partners conceivably may be modulated by intra-and extracellular conditions. This notion provokes the question of whether chromosome segregation can be regarded as a regulatory stage of the cell cycle. To address this question, we discuss how environmental conditions affect chromosome segregation and how segregation proteins influence other cell cycle processes.

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“…In model species, spatiotemporal control of cell division often invokes a negative regulator that prevents division at a given time and/or place in the cell. For instance, nucleoid occlusion factors hinder division over non-partitioned chromosomes (Wu and Errington, 2004;Bernhardt and de Boer, 2005;Mercy et al, 2019), whereas the Min system inhibits division at cell poles (Raskin and de Boer, 1997). A few proteins that positively regulate the localization of the cytokinetic protein FtsZ have also been discovered recently (Willemse et al, 2011;Treuner-Lange et al, 2013;Fleurie et al, 2014;van Raaphorst et al, 2017;Eswara et al, 2018).…”

Section: Spatiotemporal Control Of Non-binary Cell Divisionmentioning

confidence: 99%

Shedding Light on the Cell Biology of the Predatory Bacterium Bdellovibrio bacteriovorus

Laloux

2020

Front. Microbiol.

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Bdellovibrio bacteriovorus is a predatory bacterium that feeds upon and proliferates inside other Gram-negative bacteria. Upon entry into the periplasmic space of the prey envelope, B. bacteriovorus initiates an exquisite developmental program in which it digests the host resources and grows as a filament, which eventually divides in a nonbinary manner, releasing a variable number of daughter cells. The progeny then escape from the prey ghost to encounter new victims and resume the predation cycle. Owing to its unique biology, B. bacteriovorus undoubtedly represents an attractive model to unravel novel mechanisms of bacterial cell cycle control and cellular organization. Yet, the molecular factors behind the sophisticated lifestyle of this micro-predator are still mysterious. In particular, the spatiotemporal dynamics of proteins that control key cellular processes such as transmission of the genetic information, cell growth and division remain largely unexplored. In this Perspective article, I highlight outstanding fundamental questions related to these aspects and arising from the original biology of this bacterium. I also discuss available insights and potential cell biology approaches based on quantitative live imaging techniques, in combination with bacterial genetics and biochemistry, to shed light on the intracellular organization of B. bacteriovorus in space and time.Despite the general lack of intracytoplasmic organelles, bacterial cells are remarkably organized in space and time. Indeed, their content is distributed at specific subcellular locations in a sophisticated and dynamic manner. Such spatiotemporal organization allows various cellular processes to be tightly coordinated with each other, which is absolutely crucial for the proper completion of every cell cycle and for successful adaptation to the environment. The fine regulation of key Frontiers in Microbiology | www.frontiersin.org January 2020 | Volume 10 | Article 3136 Laloux Cell Biology of Bdellovibrio bacteriovorus Frontiers in Microbiology | www.frontiersin.org GL conducted literature search and review and wrote the manuscript.

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RocS drives chromosome segregation and nucleoid protection in Streptococcus pneumoniae

Cited by 28 publications

References 48 publications

Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes

Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes

Chromosome Segregation Proteins as Coordinators of Cell Cycle in Response to Environmental Conditions

Shedding Light on the Cell Biology of the Predatory Bacterium Bdellovibrio bacteriovorus

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