Rocket Immunoelectrophoresis

Walker, John M.

doi:10.1007/978-1-60327-259-9_137

Cited by 17 publications

(17 citation statements)

References 6 publications

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“…To understand the association of the content of glutamine, proline, glycine, tyrosine, serine, and valine in the Glu-A1 and Glu-B1 glutenin subunits, the content of cysteine residues in Glu-B1, and the content of arginine, isoleucine, and leucine in the Glu-B3 glutenin, with the suitability of durum wheat for pasta making and the suitability of bread wheat with good bread-making quality, the ProtParam tool ( (accessed on 5 April 2007)) was used for in silico computing of the chemical parameters of Glu-A1, Glu-B1, and Glu-B3 glutenins. The computed properties included the amino acid composition, protein molecular weight, aliphatic index (alanine, valine, isoleucine, and leucine), theoretical isoelectric point (pI), negatively and positively charged residues, hydrophobicity index, and instability index [ 53 ].…”

Section: Methodsmentioning

confidence: 99%

Association of High and Low Molecular Weight Glutenin Subunits with Gluten Strength in Tetraploid Durum Wheat (Triticum turgidum spp. Durum L.)

Al-Khayri

Alshegaihi

Mahgoub

et al. 2023

Plants

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The gluten strength and the composition of high- and low-molecular-weight glutenin subunits (HMWGSs and LMWGSs) of fifty-one durum wheat genotypes were evaluated using sodium dodecyl sulfate (SDS) sedimentation testing and SDS polyacrylamide gel electrophoresis (SDS-PAGE). This study examined the allelic variability and the composition of HMWGSs and LMWGSs in T. durum wheat genotypes. SDS-PAGE was proven to be a successful method for identifying HMWGS and LMWGS alleles and their importance in determining the dough quality. The evaluated durum wheat genotypes with HMWGS alleles 7+8, 7+9, 13+16, and 17+18 were highly correlated with improved dough strength. The genotypes containing the LMW-2 allele displayed stronger gluten than those with the LMW-1 allele. The comparative in silico analysis indicated that Glu-A1, Glu-B1, and Glu-B3 possessed a typical primary structure. The study also revealed that the lower content of glutamine, proline, glycine, and tyrosineand the higher content of serine and valine in the Glu-A1 and Glu-B1 glutenin subunits, and the higher cysteine residues in Glu-B1 and lower arginine, isoleucine, and leucine in the Glu-B3 glutenin, are associated with the suitability of durum wheat for pasta making and the suitability of bread wheat with good bread-making quality. The phylogeny analysis reported that both Glu-B1 and Glu-B3 had a closer evolutionary relationship in bread and durum wheat, while the Glu-A1 was highly distinct. The results of the current research may help breeders to manage the quality of durum wheat genotypes by exploiting the allelic variation in glutenin. Computational analysis showed the presence of higher proportions of glutamine, glycine, proline, serine, and tyrosine than the other residues in both HMWGSs and LMWGSs. Thus, durum wheat genotype selection according to the presence of a few protein components effectively distinguishes the strongest from the weakest types of gluten.

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Section: Methodsmentioning

confidence: 99%

Association of High and Low Molecular Weight Glutenin Subunits with Gluten Strength in Tetraploid Durum Wheat (Triticum turgidum spp. Durum L.)

Al-Khayri

Alshegaihi

Mahgoub

et al. 2023

Plants

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“…For competitive binding studies, a rabbit anti-GRP78/BiP antibody (Enzo Life Sciences #ADI-SPA-826F) was radiolabeled with 125 I using a modified Iodogen method as previously described. [20] Na- 125 I was obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO) and Iodogen reagent (1,3,4,6-tetrachloro-3α,6α-diphenylglycoluril) was obtained from Thermo Fisher Scientific (Rockford, IL).…”

Section: Methodsmentioning

confidence: 99%

Synergistic enhancement of cancer therapy using a combination of heat shock protein targeted HPMA copolymer–drug conjugates and gold nanorod induced hyperthermia

Larson¹,

Gormley

Frazier

et al. 2013

Journal of Controlled Release

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In the field of nanomedicine, selective delivery to cancer cells is a common goal, where active targeting strategies are often employed to increase tumor accumulation. In this study, tumor hyperthermia was utilized as a means to increase the active delivery of heat shock protein (HSP) targeted N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer-drug conjugates. Following hyperthermia, induced expression of cell surface heat shock protein (HSP) glucose regulated protein 78 kDa (GRP78) was utilized for targeted drug therapy. Conjugates bearing the anticancer agents aminohexylgeldanamycin (AHGDM), docetaxel (DOC), or cisplatin and the GRP78 targeting peptide WDLAWMFRLPVG were synthesized and characterized. Binding to cell surface expressed heat shock protein GRP78 on the surface of human prostate cancer DU145 cells was evaluated. HSP targeted AHGDM and DOC conjugates demonstrated active binding comparable to native targeting peptide. They were then assessed in vitro for the ability to synergistically induce cytotoxicity in combination with moderate hyperthermia (43°C, 30 min.). HSP targeted DOC conjugates exhibited high potency against DU145 cells with an IC50 of 2.4 nM. HSP targeted AHGDM and DOC conjugates demonstrated synergistic effects in combination with hyperthermia with combination index values of 0.65 and 0.45 respectively. Based on these results, HSP targeted DOC conjugates were selected for in vivo evaluation. In DU145 tumor bearing mice, a single treatment of tumor hyperthermia, induced via gold nanorod mediated plasmonic photothermal therapy, and intravenous administration of HSP targeted HPMA copolymer-docetaxel at 10 mg/kg resulted in maintained tumor regression for a period of 30 days. These results demonstrate the potential for tumor hyperthermia to increase the delivery of HSP targeted macromolecular chemotherapeutics.

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“…However, the hydropathy values of all amino acid residues are added, and the GRAVY is calculated by dividing the total number of residues by the results. 43,44…”

Section: Methodsmentioning

confidence: 99%

Multifaceted mutational immunotherapeutic approach to design therapeutic mAbs to combat monkeypox disease via integrated screening algorithms and antibody engineering

Singh¹,

Rao²,

Mishra³

et al. 2023

Mol. Syst. Des. Eng.

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Rocket Immunoelectrophoresis

Cited by 17 publications

References 6 publications

Association of High and Low Molecular Weight Glutenin Subunits with Gluten Strength in Tetraploid Durum Wheat (Triticum turgidum spp. Durum L.)

Association of High and Low Molecular Weight Glutenin Subunits with Gluten Strength in Tetraploid Durum Wheat (Triticum turgidum spp. Durum L.)

Synergistic enhancement of cancer therapy using a combination of heat shock protein targeted HPMA copolymer–drug conjugates and gold nanorod induced hyperthermia

Multifaceted mutational immunotherapeutic approach to design therapeutic mAbs to combat monkeypox disease via integrated screening algorithms and antibody engineering

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