Robustness and plasticity in Drosophila heat avoidance

Simões, José Miguel; Levy, Joshua I.; Zaharieva, Emanuela E.; Vinson, Leah T.; Zhao, Peixiong; Alpert, Michael H.; Kath, William L.; Para, Alessia; Gallio, Marco

doi:10.1038/s41467-021-22322-w

Cited by 21 publications

(49 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1F ). This result is consistent with previous reports (Ni et al, 2013, Simões et al, 2021, Budelli et al, 2019). Moreover, Gr28b MB also moved more than wild-type ( wt ) flies in this condition ( Fig.…”

Section: Resultssupporting

confidence: 94%

Using TrackMate to Analyze Drosophila Larval and Adult Locomotion

Huda

et al. 2021

Preprint

View full text Add to dashboard Cite

Drosophila adult and larvae exhibit sophisticated behaviors that are widely used in development, synaptic transmission, sensory physiology, and learning and memory research. Many of these behaviors depend on locomotion, the ability of an animal to move. However, the statistical analysis of locomotion is not trivial. Here we use an open-source Fiji plugin TrackMate to track the locomotion of Drosophila adults and larvae. We build optimal experimental setups to rapidly process recordings by Fiji and analyze by TrackMate. We also provide tips for analyzing non-optimal recordings. TrackMate extracts the X and Y positions of an animal on each frame of an image sequence or a video. This information allows for generating moving trajectories, calculating moving distances, and determining preference indices in two-choice assays. Notably, this free-cost analysis method does not require programming skills.

show abstract

Section: Resultssupporting

confidence: 94%

Using TrackMate to Analyze Drosophila Larval and Adult Locomotion

Huda

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…3A,B). PIs of Gr28b MB were significantly lower than that of WCS, which is consistent with previous reports (Ni et al, 2013, Simões et al, 2021, Budelli et al, 2019 (Fig. 3A,B).…”

Section: Biarmipes D Bipectinata D Mojavensis and D Yakuba Don't Avoid Warm Temperaturessupporting

confidence: 93%

“…3A,B). PIs of Gr28b MB were significantly lower than that of WCS, which is consistent with previous reports (Ni et al, 2013, Simões et al, 2021, Budelli et al, 2019 (which was not certified by peer review) is the author/funder. All rights reserved.…”

Section: Biarmipes D Bipectinata D Mojavensis and D Yakuba Don't Avoid Warm Temperaturessupporting

confidence: 92%

See 1 more Smart Citation

Responses to Temperatures of DifferentDrosophilaSpecies

Huda

Vaden

Omelchenko

et al. 2021

Preprint

View full text Add to dashboard Cite

Temperature is a critical environmental variable that affects the distribution, survival, and reproduction of most animals. Although temperature receptors have been identified in different animals, how these receptors respond to temperatures is largely unknown. Here we use modified single-fly thermotactic assays to analyze movements and temperature preferences of nine Drosophila species. The ability/inclination to move varies among these species and at different temperatures. Importantly, different species prefer various ranges of temperatures. While wild-type D. melanogaster flies avoid the warm temperature in the warm avoidance assay and the cool temperature in the cool avoidance assay, D. bipectinata and D. yakuba avoid neither warm nor cool temperatures and D. biarmipes and D. mojavensis do not avoid the warm temperature in the warm avoidance assay. These results demonstrate that Drosophila species have different mobilities and temperature preferences, thereby benefiting the research on molecular mechanisms of temperature responsiveness.

show abstract

“…Unlike adult flies who always select ∼25 • C, fly larvae search for different temperatures when confronted with gradient and sudden temperature changes. They choose ∼18 • C when exposed to a sudden temperature increase, but slowly pursue ∼25 • C on a shallow temperature gradient (Hamada et al, 2008;Kwon et al, 2008Kwon et al, , 2010Gallio et al, 2011;Shen et al, 2011;Ni et al, 2013;Sokabe et al, 2016;Frank et al, 2017;Simões et al, 2021;Tyrrell et al, 2021;Huda et al, 2022). Further studies are needed to understand why and how larvae select a low temperature in response to fast temperature variations but a higher temperature to slow changes.…”

Section: Discussionmentioning

confidence: 99%

Cool and warm ionotropic receptors control multiple thermotaxes in Drosophila larvae

Omelchenko

Bai²,

Spina³

et al. 2022

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Animals are continuously confronted with different rates of temperature variation. The mechanism underlying how temperature-sensing systems detect and respond to fast and slow temperature changes is not fully understood in fly larvae. Here, we applied two-choice behavioral assays to mimic fast temperature variations and a gradient assay to model slow temperature changes. Previous research indicates that Rhodopsin 1 (Rh1) and its phospholipase C (PLC) cascade regulate fast and slow temperature responses. We focused on the ionotropic receptors (IRs) expressed in dorsal organ ganglions (DOG), in which dorsal organ cool-activated cells (DOCCs) and warm-activated cells (DOWCs) rely on IR-formed cool and warm receptors to respond to temperature changes. In two-choice assays, both cool and warm IRs are sufficient for selecting 18°C between 18°C and 25°C but neither function in cool preferences between 25°C and 32°C. The Rh1 pathway, on the other hand, contributes to choosing preferred temperatures in both assays. In a gradient assay, cool and warm IR receptors exert opposite effects to guide animals to ∼25°C. Cool IRs drive animals to avoid cool temperatures, whereas warm IRs guide them to leave warm regions. The Rh1 cascade and warm IRs may function in the same pathway to drive warm avoidance in gradient assays. Moreover, IR92a is not expressed in temperature-responsive neurons but regulates the activation of DOWCs and the deactivation of DOCCs. Together with previous studies, we conclude that multiple thermosensory systems, in various collaborative ways, help larvae to make their optimal choices in response to different rates of temperature change.

show abstract

Robustness and plasticity in Drosophila heat avoidance

Cited by 21 publications

References 50 publications

Using TrackMate to Analyze Drosophila Larval and Adult Locomotion

Using TrackMate to Analyze Drosophila Larval and Adult Locomotion

Responses to Temperatures of DifferentDrosophilaSpecies

Cool and warm ionotropic receptors control multiple thermotaxes in Drosophila larvae

Contact Info

Product

Resources

About