Robust thin-film fluorescence thermometry for prolonged measurements in microfluidic devices

Schreiter, Kurt M.; Glawdel, Tomasz; Forrest, James A.; Ren, Carolyn L.

doi:10.1039/c3ra41368c

Cited by 8 publications

(7 citation statements)

References 27 publications

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“…Our herein presented study concerns the exploration of novel thermoluminophores for high spatial and thermal resolution thermography at temperatures around RT. In this context, we find that known thermoluminophores, namely, organic dyes, polymers, quantum dots, rare‐earth‐doped metal‐oxides, [ 17–25 ] face limitations such as complexity in materials fabrication or thin‐film deposition, durability and robustness, or unsuited optical characteristics for a specific temperature range or common detection methods.…”

Section: Figurementioning

confidence: 99%

Hybrid 0D Antimony Halides as Air‐Stable Luminophores for High‐Spatial‐Resolution Remote Thermography

et al. 2021

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Luminescent organic–inorganic low‐dimensional ns2 metal halides are of rising interest as thermographic phosphors. The intrinsic nature of the excitonic self‐trapping provides for reliable temperature sensing due to the existence of a temperature range, typically 50–100 K wide, in which the luminescence lifetimes (and quantum yields) are steeply temperature‐dependent. This sensitivity range can be adjusted from cryogenic temperatures to above room temperature by structural engineering, thus enabling diverse thermometric and thermographic applications ranging from protein crystallography to diagnostics in microelectronics. Owing to the stable oxidation state of Sb3+, Sb(III)‐based halides are far more attractive than all major non‐heavy‐metal alternatives (Sn‐, Ge‐, Bi‐based halides). In this work, the relationship between the luminescence characteristics and crystal structure and microstructure of TPP2SbBr5 (TPP = tetraphenylphosphonium) is established, and then its potential is showcased as environmentally stable and robust phosphor for remote thermography. The material is easily processable into thin films, which is highly beneficial for high‐spatial‐resolution remote thermography. In particular, a compelling combination of high spatial resolution (1 µm) and high thermometric precision (high specific sensitivities of 0.03–0.04 K−1) is demonstrated by fluorescence‐lifetime imaging of a heated resistive pattern on a flat substrate, covered with a solution‐spun film of TPP2SbBr5.

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Section: Figurementioning

confidence: 99%

Hybrid 0D Antimony Halides as Air‐Stable Luminophores for High‐Spatial‐Resolution Remote Thermography

et al. 2021

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“…The additional PDMS layer was used to protect the sensing layer and promote adhesion to the fluidic structure. Schreiter et al directly bonded the fluidic structure onto a dye-doped PS or PMMA layer [41]. They used a modified oxygen plasma based bonding procedure to achieve sealing between the temperature sensitive layer and the PDMS fluidic network.…”

Section: Coating and Sealing With Pdmsmentioning

confidence: 99%

Microfluidic platforms employing integrated fluorescent or luminescent chemical sensors: a review of methods, scope and applications

Pfeiffer

Nagl

2015

Methods Appl. Fluoresc.

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Herein we critically review microfluidic platforms that contain integrated fluorescent or luminescent chemical sensor assemblies. These were employed in particular for miniaturized oxygen and pH sensing. Microchips with optical temperature sensing capability are also covered since these share many concepts and applications. Other analytes and derived parameters from the above analytes are found in some sensing approaches in microfluidics.After an introduction, the work is structured into three main chapters dealing with the fabrication and microintegration of these sensors, readout and detection strategies, and applications of these microsystems. The fabrication is discussed with a focus on soft lithography-based approaches in polydimethylsiloxane (PDMS) or PDMS and glass hybrid devices that form the majority of work so far. Alternative approaches, particularly using glass or quartz as the main chip material are also covered. Detection techniques employed to date are the subject of the next chapter, where simple intensity as well as lifetime- or wavelength-referenced schemes are presented and the utility of image-based sensing on the microscale is discussed.Lastly, exciting applications of these microfluidic chips are highlighted. Luminescent oxygen and pH sensing has been of particular interest in the field of microbioreactors but other areas are also of interest, particularly chemical reactors and electrophoresis. Optical temperature sensing is discussed and its use in fundamental studies as well as in enzyme reactors. Integrated microsystems with biosensing capabilities and some for monitoring of metal ions and other analytes are also presented.

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“…Free probes that are not stationary and not shielded from the environment are not well-suited for that. For integration of stationary luminescent chemical sensor layers into microfluidic chips, numerous ways have been devised in recent years. − Temperature sensors had been introduced into glass and elastomer hybrid microfluidic systems by staining the elastomer with fluorescent probes, blade- or spin-coating of glass substrates, and subsequent sealing of the elastomer to the glass.…”

mentioning

confidence: 99%

On-Chip Photothermal Analyte Detection Using Integrated Luminescent Temperature Sensors

Pfeiffer

Nagl

2017

Anal. Chem.

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Optical absorbance detection based on attenuated light transmission is limited in sensitivity due to short path lengths in microfluidic and other miniaturized platforms. An alternative is detection using the photothermal effect. Herein we introduce a new kind of photothermal absorbance measurement using integrated luminescent temperature sensor spots inside microfluidic channels. The temperature sensors were photopolymerized inside the channels from NOA 81 UV-curable thiolene prepolymer doped with a tris(1,10-phenanthroline)ruthenium(II) temperature probe. The polymerized sensing structures were as small as 26 ± 3 μm in diameter and displayed a temperature resolution of better than 0.3 K between 20 and 50 °C. The absorbance from 532 nm laser excitation of the food dye Amaranth as a model analyte was quantified using these spots, and the influence of the flow rate, laser power, and concentration was investigated. Calibration yielded a linear relationship between analyte concentration and the temperature signal in the channels. The limit of detection for the azo-dye Amaranth (E123) in this setup was 13 μM. A minimal detectable absorbance of 3.2 × 10 AU was obtained using an optical path length of 125 μm in this initial study. A microreactor with integrated temperature sensors was then employed for an absorbance-based miniaturized nitrite analysis, yielding a detection limit of 26 μM at a total assay time of only 75 s. This technique is very promising for sensitive, and potentially spatially resolved, optical absorbance detection on the micro- and nanoscale.

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Robust thin-film fluorescence thermometry for prolonged measurements in microfluidic devices

Cited by 8 publications

References 27 publications

Hybrid 0D Antimony Halides as Air‐Stable Luminophores for High‐Spatial‐Resolution Remote Thermography

Hybrid 0D Antimony Halides as Air‐Stable Luminophores for High‐Spatial‐Resolution Remote Thermography

Microfluidic platforms employing integrated fluorescent or luminescent chemical sensors: a review of methods, scope and applications

On-Chip Photothermal Analyte Detection Using Integrated Luminescent Temperature Sensors

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