Robust Surface Plasmon Resonance Chips for Repetitive and Accurate Analysis of Lignin–Peptide Interactions

Isozaki, Katsuhiro; Shimoaka, Takafumi; Oshiro, Satoshi; Yamaguchi, Asako; Pincella, Francesca; Ueno, Ryo; Hasegawa, Takeshi; Watanabe, Takashi; Takaya, Hikaru; Nakamura, Masaharu

doi:10.1021/acsomega.8b01161

Cited by 7 publications

(5 citation statements)

References 68 publications

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“…With RHBL g-oxCA in hand, we decided to couple the newly introduced carboxylic acids with aniline 10,a sw ef elt this represented af lexible approach to grafting onto the lignin backbone that complemented other recent reports from our group and others. [5,[46][47][48][49][50][51][52] Initially, model 11 was synthesized from 9 and 10 to aid characterization of the final lignin (Scheme 1). RHBL g-oxCA was then coupled with 10 by using EDC, Et 3 Na nd HOBt at RT for 24 h( Lignin general procedure D).…”

Section: Tempo-mediated Formation Of B-o-4 G-carboxylic Acid-substitumentioning

confidence: 99%

Selective Primary Alcohol Oxidation of Lignin Streams from Butanol‐Pretreated Agricultural Waste Biomass

Panovic

Lancefield

Phillips³

et al. 2019

ChemSusChem

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Chemically modified lignins are important for the generation of biomass‐derived materials and as precursors to renewable aromatic monomers. A butanol‐based organosolv pretreatment has been used to convert an abundant agricultural waste product, rice husks, into a cellulose pulp and three additional product streams. One of these streams, a butanol‐modified lignin, was oxidized at the γ position to give a carboxylic acid functionalized material. Subsequent coupling of the acid with aniline aided lignin characterization and served as an example of the flexibility of this approach for grafting side chains onto a lignin core structure. The pretreatment was scaled up for use on a multi‐kilogram scale, a development that enabled the isolation of an anomeric mixture of butoxylated xylose in high purity. The robust and scalable butanosolv pretreatment has been developed further and demonstrates considerable potential for the processing of rice husks.

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Section: Tempo-mediated Formation Of B-o-4 G-carboxylic Acid-substitumentioning

confidence: 99%

Selective Primary Alcohol Oxidation of Lignin Streams from Butanol‐Pretreated Agricultural Waste Biomass

Panovic

Lancefield

Phillips³

et al. 2019

ChemSusChem

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“…The optofluidics methods mostly applied as biosensors for microfluidics measurements can be divided into two general categories: label-free and label-based methods. Label-free methods include surface plasmon resonance, 41,42 scanning electron microscopy, 43 and Raman microscopy. 44 Although these methods allow the study of specific cell's secreted/excreted compounds, cellular morphology or targeting of specific areas of individual cells for analysis, respectively, some shortcomings are intrinsically associated, namely possible interference with conformation of molecular structures, interaction with biological processes, and demand for bulky external equipment.…”

Section: Microscopy-based Biosensorsmentioning

confidence: 99%

New bioimaging avenues for organs‐on‐chips by integration of bioluminescence

Teixeira

Mezzanotte

2021

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The unmet demand for the development of advanced in vitro models that emulate key features of human (patho-)physiology and organ or tissue functionality led to the emergence of organ-on-a-chip screening platforms. This biomimetic microtechnology enables accurate prediction of human responses, even at the multitissue or organ interaction level. With these advances, the necessity of incorporating biosensing within these platform becomes increasingly evident, to ultimately allow in situ, noninvasive, monitoring of cells, tissues and organs' behavior. Seamlessly integrated biosensors on-chip also offer the prospect of uninterrupted and fully automated analysis. The continuous search for biosensors that not only avoid disruptive analyses, but are also highly sensitive and permit instant in situ evaluation, steered further developments of optical biosensors including bioluminescence (BL). BL is particularly suitable to interrogate biological systems at the multiparameter level and it is well matched to address the intrinsic biological systems' heterogeneity due to its inherent high signal to background ratio, to which the demand of simple measurement equipment can be added. Moreover, BL can be effortlessly integrated into any available organ-ona-chip platform and provides unique groundbreaking means for online monitoring, at the cellular level, and/or detection of cell secreted/excreted compounds. This review depicts recent advances in biosensors, with particular focus on BL. We highlight current challenges and future directions, aimed at stimulating the application of BL to interface organ-on-a-chip systems, leading to the exponential advancement of both fields.

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“…Lignin-modified SPR probes have been successfully employed for the study of the binding and precipitation behaviour of glycinin and b-conglycinin from soy beans (Salas et al 2013). An SPR probe modified with milled wood lignin immobilized onto a self-assembled monolayer of methoxypolyethylene glycol thiol (mPEG-SH) was used to investigate the binding of 12-mer peptides to lignin (Yamaguchi et al 2016;Isozaki et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Non-productive binding of cellobiohydrolase i investigated by surface plasmon resonance spectroscopy

et al. 2021

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Future biorefineries are facing the challenge to separate and depolymerize biopolymers into their building blocks for the production of biofuels and basic molecules as chemical stock. Fungi have evolved lignocellulolytic enzymes to perform this task specifically and efficiently, but a detailed understanding of their heterogeneous reactions is a prerequisite for the optimization of large-scale enzymatic biomass degradation. Here, we investigate the binding of cellulolytic enzymes onto biopolymers by surface plasmon resonance (SPR) spectroscopy for the fast and precise characterization of enzyme adsorption processes. Using different sensor architectures, SPR probes modified with regenerated cellulose as well as with lignin films were prepared by spin-coating techniques. The modified SPR probes were analyzed by atomic force microscopy and static contact angle measurements to determine physical and surface molecular properties. SPR spectroscopy was used to study the activity and affinity of Trichoderma reesei cellobiohydrolase I (CBHI) glycoforms on the modified SPR probes. N-glycan removal led to no significant change in activity or cellulose binding, while a slightly higher tendency for non-productive binding to SPR probes modified with different lignin fractions was observed. The results suggest that the main role of the N-glycosylation in CBHI is not to prevent non-productive binding to lignin, but probably to increase its stability against proteolytic degradation. The work also demonstrates the suitability of SPR-based techniques for the characterization of the binding of lignocellulolytic enzymes to biomass-derived polymers. Graphic abstract

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Robust Surface Plasmon Resonance Chips for Repetitive and Accurate Analysis of Lignin–Peptide Interactions

Cited by 7 publications

References 68 publications

Selective Primary Alcohol Oxidation of Lignin Streams from Butanol‐Pretreated Agricultural Waste Biomass

Selective Primary Alcohol Oxidation of Lignin Streams from Butanol‐Pretreated Agricultural Waste Biomass

New bioimaging avenues for organs‐on‐chips by integration of bioluminescence

Non-productive binding of cellobiohydrolase i investigated by surface plasmon resonance spectroscopy

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