This study investigates the robust stability analysis of an unstable second order plus time-delay (SOPTD) plant by using Fractional-Order Proportional Integral (FOPI) controllers. We assume that there are simultaneous uncertainties in gain, time-constants, and time-delay of the plant. At first, a graphical method is provided for a robust stability analysis of the closed-loop system. Then, a robust stability checking function is introduced to facilitate the robust stability analysis. Additionally, new bounds are presented to reduce the computational burden for the robust stability analysis. Finally, two examples are provided to show the correctness of the proposed method.