Robust biodegradation of naproxen and diclofenac by laccase immobilized using electrospun nanofibers with enhanced stability and reusability

Zdarta, Jakub; Jankowska, Katarzyna; Wyszowska, Marta; Kijeńska‐Gawrońska, Ewa; Zgoła‐Grześkowiak, Agnieszka; Pinelo, Manuel; Meyer, Anne S.; Moszyński, Dariusz; Jesionowski, Teofil

doi:10.1016/j.msec.2019.109789

Cited by 92 publications

(55 citation statements)

References 37 publications

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“…However, it should be noted that due to the open structure of the chitinous matrix and limited deformation of the enzyme structure, only a slight worsening of catalytic properties was observed. Similar observations were made in our previous study concerning adsorption immobilization of laccase on poly(l-lactic acid)-co-poly(ε-caprolactone) electrospun nanofibers [63]. By contrast, in a study by Olajuyigbe et al [64], laccase was immobilized by entrapment using calcium and copper alginate beads.…”

Section: Characterization Of Products Following Immobilizationsupporting

confidence: 85%

3D Chitin Scaffolds from the Marine Demosponge Aplysina archeri as a Support for Laccase Immobilization and Its Use in the Removal of Pharmaceuticals

et al. 2020

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For the first time, 3D chitin scaffolds from the marine demosponge Aplysina archeri were used for adsorption and immobilization of laccase from Trametes versicolor. The resulting chitin–enzyme biocatalytic systems were applied in the removal of tetracycline. Effective enzyme immobilization was confirmed by scanning electron microscopy. Immobilization yield and kinetic parameters were investigated in detail, in addition to the activity of the enzyme after immobilization. The designed systems were further used for the removal of tetracycline under various process conditions. Optimum process conditions, enabling total removal of tetracycline from solutions at concentrations up to 1 mg/L, were found to be pH 5, temperature between 25 and 35 °C, and 1 h process duration. Due to the protective effect of the chitinous scaffolds and stabilization of the enzyme by multipoint attachment, the storage stability and thermal stability of the immobilized biomolecules were significantly improved as compared to the free enzyme. The produced biocatalytic systems also exhibited good reusability, as after 10 repeated uses they removed over 90% of tetracycline from solution. Finally, the immobilized laccase was used in a packed bed reactor for continuous removal of tetracycline, and enabled the removal of over 80% of the antibiotic after 24 h of continuous use.

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Section: Characterization Of Products Following Immobilizationsupporting

confidence: 85%

3D Chitin Scaffolds from the Marine Demosponge Aplysina archeri as a Support for Laccase Immobilization and Its Use in the Removal of Pharmaceuticals

et al. 2020

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“…Degradation of EDCs using immobilized laccase has been the subject of a number of studies [55,56]. Cerrena unicolor laccase (12 U/5 mL) captured on porous silica beads, for example, has been shown to eliminate around 90% of BPA (50 µM) after 60 min of incubation [57].…”

Section: Resultsmentioning

confidence: 99%

Polyamide-Laccase Nanofiber Membrane for Degradation of Endocrine-Disrupting Bisphenol A, 17α-ethinylestradiol, and Triclosan

et al. 2019

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Contamination of potable water by endocrine disrupting chemicals (EDCs) is a growing problem worldwide. One of the possible treatments is the utilization of laccase enzyme catalyzing oxidation of phenolic structures of EDC when anchored in a polymeric nanofiber membrane. Previous studies failed to develop a membrane with a sufficiently active enzyme, or the immobilization process was too complicated and time-consuming. Here, we established an elegant method for immobilizing Trametes versicolor laccase onto polyamide 6 nanofibers (PA6-laccase) via adsorption and glutaraldehyde crosslinking, promoting high enzyme activity and easier applicability in water treatment technology. This simple and inexpensive immobilization ensures both repeated use, with over 88% of initial activity retained after five ABTS catalytic cycles, and enhanced storage stability. PA6-laccase was highly effective in degrading a 50-µM EDC mixture, with only 7% of bisphenol A, 2% of 17α-ethinylestradiol, and 30% of triclosan remaining after a 24-h catalytic process. The PA6-laccase membrane can lead to the improvement of novel technologies for controlling of EDC contamination in potable water.

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“…However, they observed that after 24 h, the EC 30 (the concentration of the drug at which 30% of the microorganisms showed a positive response after the exposure time) values for untreated naproxen and diclofenac solutions amounted around 20% and 25%, respectively. Enzymatic treatment with encapsulated laccase resulted in decreasing the EC 30 values to around 80% and 85% for diclofenac and naproxen, respectively (Zdarta et al 2019). A decrease in the toxicity was also observed by Marco-Urrea et al (2010) and Rodriguez-Rodriguez et al (2011) during the treatment of naproxen effluent using Trametes versicolor.…”

Section: Occurrence Of Naproxen In the Environmentmentioning

confidence: 86%

“…Although low concentrations of naproxen occur in the environment, the increase in its toxicity may be related to a synergy effect with other contaminations. Zdarta et al (2019) examined the toxicity of untreated and enzymatically treated solutions of naproxen and diclofenac against Artemia salina. However, they observed that after 24 h, the EC 30 (the concentration of the drug at which 30% of the microorganisms showed a positive response after the exposure time) values for untreated naproxen and diclofenac solutions amounted around 20% and 25%, respectively.…”

Section: Occurrence Of Naproxen In the Environmentmentioning

confidence: 99%

Naproxen in the environment: its occurrence, toxicity to nontarget organisms and biodegradation

Wojcieszyńska

Guzik

2020

Appl Microbiol Biotechnol

110

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This article summarizes the current knowledge about the presence of naproxen in the environment, its toxicity to nontarget organisms and the microbial degradation of this drug. Currently, naproxen has been detected in all types of water, including drinking water and groundwater. The concentrations that have been observed ranged from ng/L to μg/L. These concentrations, although low, may have a negative effect of long-term exposure on nontarget organisms, especially when naproxen is mixed with other drugs. The biological decomposition of naproxen is performed by fungi, algae and bacteria, but the only well-described pathway for its complete degradation is the degradation of naproxen by Bacillus thuringiensis B1(2015b). The key intermediates that appear during the degradation of naproxen by this strain are O-desmethylnaproxen and salicylate. This latter is then cleaved by 1,2-salicylate dioxygenase or is hydroxylated to gentisate or catechol. These intermediates can be cleaved by the appropriate dioxygenases, and the resulting products are incorporated into the central metabolism. Key points •High consumption of naproxen is reflected in its presence in the environment. •Prolonged exposure of nontargeted organisms to naproxen can cause adverse effects. •Naproxen biodegradation occurs mainly through desmethylnaproxen as a key intermediate.

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Robust biodegradation of naproxen and diclofenac by laccase immobilized using electrospun nanofibers with enhanced stability and reusability

Cited by 92 publications

References 37 publications

3D Chitin Scaffolds from the Marine Demosponge Aplysina archeri as a Support for Laccase Immobilization and Its Use in the Removal of Pharmaceuticals

3D Chitin Scaffolds from the Marine Demosponge Aplysina archeri as a Support for Laccase Immobilization and Its Use in the Removal of Pharmaceuticals

Polyamide-Laccase Nanofiber Membrane for Degradation of Endocrine-Disrupting Bisphenol A, 17α-ethinylestradiol, and Triclosan

Naproxen in the environment: its occurrence, toxicity to nontarget organisms and biodegradation

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