Listening to music is an enjoyable activity for most individuals, yet the factors that relate to aesthetic preferences are not completely understood. In the present article, we investigate whether the absolute tuning of music influences listener evaluations of music. Across three experiments, participants rated musical excerpts, tuned conventionally (A4 = 440 Hz) versus unconventionally (+50 cents from conventional tuning), in terms of aesthetic preference. In Experiment 1, participants rated single musical instrument digital interface piano excerpts on each trial in terms of liking, interest, and unusualness. In Experiments 2 and 3, participants heard two versions of the same excerpt on each trial, only differing in terms of tuning, and made a forced-choice judgment as to which version they preferred. Experiment 2 used the same piano excerpts as Experiment 1, whereas Experiment 3 introduced both highly familiar and unknown song excerpts by professional recording artists. Overall, the results suggest that absolute tuning influences aesthetic preferences under limited circumstances. Although there was no strong evidence for tuning influencing judgments in either Experiments 1 or 2, we found a robust effect in Experiment 3 depending on the familiarity of the recording. Whereas participants clearly preferred the conventionally tuned version for highly familiar recordings, they tended to prefer the version that was highest in absolute pitch if the recording was unfamiliar. Overall, these results suggest that absolute tuning can influence musical preferences, although the specific nature of the effect depends on familiarity.