This paper investigates the design of a missile seeker servo system combined with a guidance and control system. Firstly, a complete model containing a missile seeker servo system, missile guidance system, and missile control system (SGCS) was creatively proposed. Secondly, a designed high-order tracking differentiator (HTD) was used to estimate states of systems in real time, which guarantees the feasibility of the designed algorithm. To guarantee tracking precision and robustness, backstepping sliding-mode control was adopted. Aiming at the main problem of projectile motion disturbance, an adaptive radial basis function neural network (RBFNN) was proposed to compensate for disturbance. Adaptive RBFNN especially achieves online adjustment of residual error, which promotes estimation precision and eliminates the “chattering phenomenon”. The boundedness of all signals, including estimation error of high-order tracking differentiator, was especially proved via the Lyapunov stability theory, which is more rigorous. Finally, in considered scenarios, line of sight angle (LOSA)-tracking simulations were carried out to verify the tracking performance, and a Monte Carlo miss-distance simulation is presented to validate the effectiveness of the proposed method.