Robo2-Slit1 dependent cell-cell interactions mediate assembly of the trigeminal ganglion

Clustering Genes, Tissues, Cells and Bioactive Chemicals by Sphere SOM

“…2 and the candidate genes clustered in each spot are listed in Table 1. It is noteworthy that ErbB3 and ROBO2 have been nominated as potential diagnostic markers here and some reports are found describing their relationships with breast cancers (Lemoine et al, 1992;Gasparini, 1994;Quinn et al 1994Travis et al, 1996Naidu et al, 1998;Fogel et al, 1999;Holbro et al, 2003;Barnes et al, 2005;Schabath et al, 2006;Shiau et al 2008). MUC1 is also known as a diagnostic marker in various cancers including breast cancers (Singh et al, 2008).…”

Mechanisms of cranial placode assembly

“…Close proximity between the two cell types has been shown in a number of studies at several stages of gangliogenesis (for instance, see Begbie, 2001;Shiau et al, 2008;Shigetani et al, 2008;Theveneau et al, 2013), the most recent one reporting the intriguing observation that NCC form corridors that wrap around migrating epibranchial neuroblasts in chick and mouse (Freter et al, 2013). Contradictory results were obtained from cell ablation experiments attempting to unravel a functional interaction between the two cell populations.…”

“…NCC express the Slit1 ligand, whereas placodal cells express its cognate receptor Robo2, and functional perturbation of this ligand-receptor pair leads to disorganised trigeminal ganglia (Shiau et al, 2008). The same authors further showed that N-Cadherin acts in cooperation with Slit1/Robo2 signalling to mediate coalescence of the trigeminal ganglion (Shiau and Bronner-Fraser, 2009).…”

“…The delamination process of trigeminal and epibranchial placodes was initially observed on sections of fixed tissues in mammals and in chick embryos (for example, see Blentic et al, 2011;Graham et al, 2007;Knabe et al, 2009;McCabe et al, 2009;Shiau et al, 2008;Xu et al, 2008), and by DiI labelling of the surface ectoderm in chick (Stark et al, 1997;Begbie, 2001a;McCabe et al, 2009). However, delamination of trigeminal or epibranchial cells from the ectoderm has to our knowledge not yet been directly observed in zebrafish or amphibian embryos, and its timing and modalities remain unclear in these species.…”

“…Contradictory results were obtained from cell ablation experiments attempting to unravel a functional interaction between the two cell populations. Whereas NCC depletion had no consequence on the development of the facial (geniculate) ganglion in mice (Coppola et al, 2010), NCC ablation in chick perturbed the coalescence of cranial ganglia without affecting delamination of placodal cells (Shiau et al, 2008), or led to early delamination defects in another study (Begbie, 2001). A recent report demonstrated with genetic ablation of subtypes of NCC that chondrogenic NCC, but not gliogenic NCC, are required for initial epibranchial ganglia formation in zebrafish (Culbertson et al, 2011).…”