Road Runoff Characterization: Ecotoxicological Assessment Combined with (Non-)Target Screenings of Micropollutants for the Identification of Relevant Toxicants in the Dissolved Phase

Abstract

Road runoff (RR) is an important vector of micropollutants towards groundwater and soils, threatening the environment and ecosystems. Through combined chemical and biological approaches, the purpose of this study was to get insights on specific toxicants present in RR from two sites differing by their traffic intensity and their toxicological risk assessment. Non-target screening was performed by HRMS on RR dissolved phase. Ecotoxicological risk was evaluated in a zebrafish embryos model and on rat liver mitochondrial respiratory chain. Specific HRMS fingerprints were obtained for each site, reflecting their respective traffic intensities. Several micropollutants, including 1,3-diphenylguanidine (DPG) and benzotriazole (BZT) were identified in greater concentrations at the high-traffic site. The origin of DPG was confirmed by analyzing HRMS fingerprints from shredded tires. RR samples from each site, DPG and BZT were of relatively low toxicity (no mortality) to zebrafish embryos, but all generated distinct and marked stress responses in the light–dark transition test, while DPG/BZT mixes abolished this effect. The moderate-traffic RR and DPG inhibited mitochondrial complex I. Our study highlights (i) the unpredictability of pollutants cocktail effect and (ii) the importance of a multi-approaches strategy to characterize environmental matrices, essential for their management at the source and optimization of depollution devices.

Polymer Additives to Go? Occurrence of the Rubber Additive 1,3-Diphenylguanidine (DPG) in Bottled Water

“…Recently, one such polymer additive transformation product, 6PPD-quinone, has been associated with the mass mortality of fish species . Other additives such as 1,3-diphenylguanidine (DPG) are also found to occur in many drinking water sources due to urban runoff events and leaching from tire materials, with DPG being detected consistently among the highest concentrations for tire leachable organics. − Additionally, DPG and 6-PPDQ uses in different applications can also be significant sources to the environment and to human exposure. , In tap water samples, DPG was found with a 100% detection frequency with a median concentration of 4.3 ng/L and was also associated with nontire-related source materials present in plumbing systems . In human urine, DPG was recently found with a detection frequency of 73% and 20% for children and adults, respectively, and at a median concentration of 0.05 ng/mL for children’s samples …”

Unveiling the Potential: Selecting Optimal Materials for Physical Pools in a Pavement-Runoff-Integrated Treatment System

“…These pollutants can have negative effects on human and animal health if they reach high concentrations. Additionally, pavement runoff [6] has also been recognized as an important source of micropollutants in groundwater. These micropollutants not only have ecotoxic effects on aquatic organisms but also negatively impact water biodiversity.…”