Ro/SS-A and the pathogenic significance of its antibodies

Serological cross-reactivity between a human Ro/SS-A autoantigen (calreticulin) and the lambda Ral-1 antigen of Onchocerca volvulus.

“…Anti-Ro/ SS-A antibodies have been defined in part by their ability to immunoprecipitate four small RNAs, hYl-hY5 (hY2 is a degraded form of hYl), from HeLa cells (4,11). Therefore, to further confirm that the protein recognized by the onchocerciasis sera was indeed a Ro/SS-A autoantigen, we performed electrophoresis of the nucleic acids from immunoprecipitates derived from WiL-2 cells labeled in vivo with [32P]orthophosphate.…”

“…Autoantibodies to the Ro/SS-A ribonucleoproteins have been associated with several autoimmune diseases, including primary Sjogren's syndrome (SS),' subacute cutaneous lupus erythematosus (SCLE), neonatal lupus erythematosus, antinuclear antibody negative systemic lupus erythematosus (SLE), and systemic lupus erythematosus-like disease secondary to homozygous C2 or C4 complement deficiency (1)(2)(3)(4)(5)(6). A considerable body ofevidence now suggests that there are at least four immunologically distinct Ro/SS-A molecules: 2 of 60 kD mol mass, as well as 52-kD and 54-kD proteins (7)(8)(9)(10).…”

Molecular cloning, expression, and chromosome 19 localization of a human Ro/SS-A autoantigen.

“…6) predicts the location of several potential epitopes including the previously characterized epitope at the amino terminus of this polypeptide, (synthetic peptide [6][7][8][9][10][11][12][13][14][15][16][17][18][19] (14), and a recently characterized epitope (synthetic peptide Figure 3. The 1.9-kb Ro cDNA nucleic acid and encoded amino acid sequence.…”

“…The significance of these antibodies is uncertain, but there is substantial evidence that they have a major role in the pathogenesis of disease (7).…”

A novel tumour associated leucine zipper protein targeting to sites of gene transcription and splicing

“…Immuno¯uorescence microscopy revealed the formation of speckles, which are con®ned to the nucleus and organized in small groups (Figure 4a). To gain a better understanding of the supramolecular structures in which this protein obviously organized itself, immunostainings with well-characterized sera from patients diagnosed for autoimmune diseases and containing autoantibodies to centromers (Kallenberg, 1990), U3-RNP/®brillarin (Ochs et al, 1985), SP100 (Szostecki et al, 1987), SS-A/Ro (McCauli e et al, 1989), SS-B/La (Chan et al, 1989) and Coilin (Andrade et al, 1991) were performed. No signi®cant co-staining with eGFP-tagged CT-8/HOM-TES-85 was obtained with these sera.…”