RNF20 and USP44 Regulate Stem Cell Differentiation by Modulating H2B Monoubiquitylation

Abstract

In Figure S4A of the above article, the authors inadvertently inserted an incorrect GAPDH panel. A revised figure, including the correct GAPDH panel, is now shown below. In addition, as the analysis shows effects of different siRNAs on the level of H2Bub1, the authors believe that the total H2B panel (identical to the previous figure) is the more appropriate control. The authors apologize for any confusion that this may have caused.

The Deubiquitylating Enzyme USP44 Counteracts the DNA Double-strand Break Response Mediated by the RNF8 and RNF168 Ubiquitin Ligases

“…Our finding that USP44 promotes histone H2A deubiquitylation is well aligned with a recent study showing that USP44 deubiquitylates histone H2B to regulate gene expression during stem cell differentiation (36). Other recent studies point to a role for USP44 as a tumor suppressor that counteracts the activity of the anaphase-promoting complex and regulates centrosome positioning during mitosis (38,42).…”

The U4/U6 Recycling Factor SART3 Has Histone Chaperone Activity and Associates with USP15 to Regulate H2B Deubiquitination

“…In contrast, many histone DUBs identified to date are promiscuous toward these functionally distinct substrates. SAGA DUB , Usp44, and Usp3 have all been reported to deubiquitinate both H2A and H2B (51)(52)(53)(54), suggesting that targeting of these DUBs is the main factor in determining specificity. Usp15 binds to ub*H2A and ub*H2B mimics with similar affinities and deubiquitinates both ubH2A and ubH2B efficiently (Figs.…”

The Epigenetic Modifier Ubiquitin-specific Protease 22 (USP22) Regulates Embryonic Stem Cell Differentiation via Transcriptional Repression of Sex-determining Region Y-box 2 (SOX2)

“…In order to assess changes in USP22 levels in response to a lineage-specific differentiation signal, RA was used to induce neuronal differentiation in mouse ESCs (5,41,42). Cells were withdrawn from 2i/LIF and differentiated with RA over the course of 5 days.…”