RNF152, a novel lysosome localized E3 ligase with pro-apoptotic activities

Zhang, Songling; Wu, Wei; Wu, You; Zheng, Jiyan; Suo, Talin; Tang, Hong; Tang, Jie

doi:10.1007/s13238-010-0083-1

Cited by 34 publications

(39 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ring finger protein 152 (RNF152) is an E3 ubiquitin ligase with a RING domain and a transmembrane (TM) domain. Previous studies have suggested that RNF152 promotes apoptosis [18] and negatively regulates mTORC1 signaling by mediating K63-linked polyubiquitination of RagA [19]. In this study, we demonstrate that RNF152 positively regulates TLR/IL-1R-mediated inflammatory response by facilitating oligomerization of MyD88, which subsequently promotes the assembly of Myddosome.…”

Section: Introductionsupporting

confidence: 62%

RNF 152 positively regulates TLR / IL ‐1R signaling by enhancing MyD88 oligomerization

Xiong

et al. 2020

EMBO Reports

View full text Add to dashboard Cite

Toll‐like receptors (TLRs) are important pattern recognition receptors (PRRs) that are critical for the defense against invading pathogens. IL‐1β is an important pro‐inflammatory cytokine that also plays pivotal roles in shaping the adaptive immune response. TLRs and interleukin‐1 receptor (IL‐1R) share similar cytosolic domains and signaling processes. In this study, we identify the E3 ubiquitin ligase RNF152 as a positive regulator of TLR/IL‐1R‐mediated signaling. Overexpression of RNF152 potentiates IL‐1β‐ and LPS‐induced NF‐κB activation in an ubiquitination‐independent manner, whereas knockdown of RNF152 has the opposite effects. RNF152‐deficient mice produce less inflammatory cytokines in response to LPS and are more resistant to LPS‐induced lethal endotoxemia. Mechanistically, RNF152 interacts with the adaptor protein MyD88 and enhances oligomerization of MyD88, which is essential for the recruitment of downstream signaling components and activation of TLR/IL‐1R‐mediated signal transduction. Our findings suggest that RNF152‐mediated oligomerization of MyD88 is important for TLR/IL‐1R‐mediated inflammatory response.

show abstract

Section: Introductionsupporting

confidence: 62%

RNF 152 positively regulates TLR / IL ‐1R signaling by enhancing MyD88 oligomerization

Xiong

et al. 2020

EMBO Reports

View full text Add to dashboard Cite

show abstract

“…For example, a protein conjugated to a polyubiquitin chain by ubiquitin lysine 48 (but possibly by all non-lysine 63 linkage) is targeted to the 26S proteasome to be degraded [ 80 ]. In our microarray results, one E3 ubiquitin-protein ligase mediating 'Lys–48'-linked polyubiquitination of target proteins (RING finger protein 152 (RNF152)) [ 82 ] was up-regulated in FW ionocytes. Furthermore, two non-ATPase regulatory subunits of the 26S proteasome were concomitantly more expressed (PSMD5 and PSMD 7).…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Analysis of Trout Gill Ionocytes in Fresh Water and Sea Water Using Laser Capture Microdissection Combined with Microarray Analysis

et al. 2015

View full text Add to dashboard Cite

Fish gills represent a complex organ composed of several cell types that perform multiple physiological functions. Among these cells, ionocytes are implicated in the maintenance of ion homeostasis. However, because the ionocyte represents only a small percent of whole gill tissue, its specific transcriptome can be overlooked among the numerous cell types included in the gill. The objective of this study is to better understand ionocyte functions by comparing the RNA expression of this cell type in freshwater and seawater acclimated rainbow trout. To realize this objective, ionocytes were captured from gill cryosections using laser capture microdissection after immunohistochemistry. Then, transcriptome analyses were performed on an Agilent trout oligonucleotide microarray. Gene expression analysis identified 108 unique annotated genes differentially expressed between freshwater and seawater ionocytes, with a fold change higher than 3. Most of these genes were up-regulated in freshwater cells. Interestingly, several genes implicated in ion transport, extracellular matrix and structural cellular proteins appeared up-regulated in freshwater ionocytes. Among them, several ion transporters, such as CIC2, SLC26A6, and NBC, were validated by qPCR and/or in situ hybridization. The latter technique allowed us to localize the transcripts of these ion transporters in only ionocytes and more particularly in the freshwater cells. Genes involved in metabolism and also several genes implicated in transcriptional regulation, cell signaling and the cell cycle were also enhanced in freshwater ionocytes. In conclusion, laser capture microdissection combined with microarray analysis allowed for the determination of the transcriptional signature of scarce cells in fish gills, such as ionocytes, and aided characterization of the transcriptome of these cells in freshwater and seawater acclimated trout.

show abstract

“…In the human ewing sarcoma cell line, PRKCD could activate mTOR through phosphorylation and inactivation of TSC2 [103], while the TOR pathway has been identified to be a regulator of the cell apoptosis and autophagy in chicken small intestines induced by arsenic [104] and a key player linking specific extracellular milieu and intracellular metabolism during Salmonella infection in chicken [105]. The downregulated ring finger protein 152 (RNF152) predicted to be regulated by the putative downregulated lncRNA NONGGAT004163.2 was localized in lysosomes and could prompt apoptosis of Hela cells [106]. In addition, 1453 mRNAs were also predicted to be trans-regulated by 87 lncRNAs.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of differentially expressed profiles of mRNAs, lncRNAs and circRNAs in chickens during Eimeria necatrix infection

et al. 2020

View full text Add to dashboard Cite

Background: Eimeria necatrix, the most highly pathogenic coccidian in chicken small intestines, can cause high morbidity and mortality in susceptible birds and devastating economic losses in poultry production, but the underlying molecular mechanisms in interaction between chicken and E. necatrix are not entirely revealed. Accumulating evidence shows that the long-non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) are key regulators in various infectious diseases. However, the expression profiles and roles of these two non-coding RNAs (ncRNAs) during E. necatrix infection are still unclear. Methods: The expression profiles of mRNAs, lncRNAs and circRNAs in mid-segments of chicken small intestines at 108 h post-infection (pi) with E. necatrix were analyzed by using the RNA-seq technique. Results: After strict filtering of raw data, we putatively identified 49,183 mRNAs, 818 lncRNAs and 4153 circRNAs. The obtained lncRNAs were classified into four types, including 228 (27.87%) intergenic, 67 (8.19%) intronic, 166 (20.29%) anti-sense and 357 (43.64%) sense-overlapping lncRNAs; of these, 571 were found to be novel. Five types were also predicted for putative circRNAs, including 180 exonic, 54 intronic, 113 antisense, 109 intergenic and 3697 senseoverlapping circRNAs. Eimeria necatrix infection significantly altered the expression of 1543 mRNAs (707 upregulated and 836 downregulated), 95 lncRNAs (49 upregulated and 46 downregulated) and 13 circRNAs (9 upregulated and 4 downregulated). Target predictions revealed that 38 aberrantly expressed lncRNAs would cis-regulate 73 mRNAs, and 1453 mRNAs could be trans-regulated by 87 differentially regulated lncRNAs. Additionally, 109 potential sponging miRNAs were also identified for 9 circRNAs. GO and KEGG enrichment analysis of target mRNAs for lncRNAs, and sponging miRNA targets and source genes for circRNAs identified associations of both lncRNAs and circRNAs with host immune defense and pathogenesis during E. necatrix infection. Conclusions: To the best of our knowledge, the present study provides the first genome-wide analysis of mRNAs, lncRNAs and circRNAs in chicken small intestines infected with E. necatrix. The obtained data will offer novel clues for exploring the interaction mechanisms between chickens and Eimeria spp.

show abstract

RNF152, a novel lysosome localized E3 ligase with pro-apoptotic activities

Cited by 34 publications

References 26 publications

RNF 152 positively regulates TLR / IL ‐1R signaling by enhancing MyD88 oligomerization

RNF 152 positively regulates TLR / IL ‐1R signaling by enhancing MyD88 oligomerization

Transcriptomic Analysis of Trout Gill Ionocytes in Fresh Water and Sea Water Using Laser Capture Microdissection Combined with Microarray Analysis

Genome-wide analysis of differentially expressed profiles of mRNAs, lncRNAs and circRNAs in chickens during Eimeria necatrix infection

Contact Info

Product

Resources

About