RNASeq in <i>C. elegans</i> Following Manganese Exposure

Parmalee, Nancy L.; Maqbool, Shahina; Ye, Bin; Calder, Brent; Bowman, Aaron B.; Aschner, Michael

doi:10.1002/0471140856.tx1120s65

Cited by 4 publications

(3 citation statements)

References 42 publications

(41 reference statements)

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“…In combination, these assays are even more powerful. For example, Parmalee and colleagues have characterized a robust method to perform RNA-Seq in C. elegans exposed to manganese, which could be easily adaptable for any environmental exposure [ 73 ]. Combined with the relative ease of genetic manipulation in C. elegans , platforms such as these are ideal for amassing gene-environment information relative to disease mechanisms in PD, like mitochondria [ 74, 75 ] and monoamine function [ 76 ].…”

Section: Pre-clinical and Basic Research: From Molecular Biology To A...mentioning

confidence: 99%

Preventing Parkinson’s Disease: An Environmental Agenda

Miranda

Goldman

Miller

et al. 2022

JPD

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Fueled by aging populations and continued environmental contamination, the global burden of Parkinson’s disease (PD) is increasing. The disease, or more appropriately diseases, have multiple environmental and genetic influences but no approved disease modifying therapy. Additionally, efforts to prevent this debilitating disease have been limited. As numerous environmental contaminants (e.g., pesticides, metals, industrial chemicals) are implicated in PD, disease prevention is possible. To reduce the burden of PD, we have compiled preclinical and clinical research priorities that highlight both disease prediction and primary prevention. Though not exhaustive, the “PD prevention agenda” builds upon many years of research by our colleagues and proposes next steps through the lens of modifiable risk factors. The agenda identifies ten specific areas of further inquiry and considers the funding and policy changes that will be necessary to help prevent the world’s fastest growing brain disease.

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Section: Pre-clinical and Basic Research: From Molecular Biology To A...mentioning

confidence: 99%

Preventing Parkinson’s Disease: An Environmental Agenda

Miranda

Goldman

Miller

et al. 2022

JPD

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“…The J1-stage worms can be transferred to seeded plates to continue development until the desired stage. The synchronization process summarized below has been adapted from previously described methods (e.g., Lewis & Fleming, 1995; see Current Protocols article; Parmalee et al, 2015). 2.…”

Section: Synchronization Of C Elegans By Using a Bleaching Solutionmentioning

confidence: 99%

Measurement of the Effects of Metals on Taxis‐to‐Food Behavior in Caenorhabditis elegans

et al. 2021

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Chemosensation in nematodes is linked to processes that affect their ability to survive, such as the search for food and the avoidance of toxic substances. Since the 1970s, numerous studies have assessed chemotaxis in the nematode species Caenorhabditis elegans, focusing on a multitude of agents, including bacteria (food), ions, salts, hormones, volatile organic compounds, and, to a lesser extent, metal-contaminated medium/food. The few studies evaluating metal exposure have reported a variety of responses (neutral, attraction, avoidance), which generally appear to be contaminant and/or concentration specific. Differences in experimental designs, however, hinder appropriate comparison of the findings and attainment of firm conclusions. Therefore, we herein propose and describe a detailed protocol for the assessment of the effects of metals on taxis-tofood behavior in C. elegans. Distinct approaches are proposed in two innovative stages of testing to (1) screen metals' effects on taxis-to-food behavior and (2) classify the behavioral response as attraction/avoidance/indifference or preference. Use of such a standard protocol will allow for easy comparison across studies and direct interpretation of results. Findings using this model system can contribute to a deeper understanding of the real risks of metal contamination to nematodes and how such contaminants could impact ecosystems in general, given the key environmental roles that these organisms play. © 2021 Wiley Periodicals LLC.Basic Protocol: Assessing the effects of metal contamination on taxis-to-food behavior in Caenorhabditis elegans Support Protocol 1: Synchronization of C. elegans by hand-picking gravid worms Support Protocol 2: Synchronization of C. elegans by using a bleaching solution

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“…In order to understand the overall toxic effects of DON in C. elegans , we employed RNAseq to analyze the comprehensive modulation of gene expression in C. elegans upon DON intoxication. RNAseq analysis has been used to elucidate transcriptional changes following manganese exposure in C. elegans [ 14 ]. Whole genome microarray analysis has similarly been used to profile the host response in mouse brain following exposure to the plant toxin abrin [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of C. elegans Reveals Novel Targets for DON Cytotoxicity

Zhang

Lawton

2018

Toxins

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Deoxynivalenol (DON) is a mycotoxin produced by Fusarium spp. that causes Fusarium head blight (FHB) disease in cereal crops. Ingestion of food contaminated with DON poses serious human health complications. However, the DON cytotoxicity has been mostly deduced from animal studies. In this study, we used the nematode Caenorhabditis elegans (C. elegans) as a tractable animal model to dissect the toxic effect of DON. Our results indicate that DON reduces the fecundity and lifespan of C. elegans. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis showed that DON upregulates innate immunity-related genes including C17H12.8 and K08D8.5 encoding PMK-1 (mitogen activated protein kinase-1)-regulated immune effectors, and F35E12.5 encoding a CUB-like domain-containing protein. Furthermore, our RNAseq data demonstrate that out of ~17,000 C. elegans genes, 313 are upregulated and 166 were downregulated by DON treatment. Among the DON-upregulated genes, several are ugt genes encoding UDP-glucuronosyl transferase (UGTs) which are known to be involved in chemical detoxification. The three upregulated genes, F52F10.4 (oac-32), C10H11.6 (ugt-26) and C10H11.4 (ugt-28) encoding the O-acyltransferase homolog, UGT26 and UGT 28, respectively, are shown to contribute to DON tolerance by a RNAi bacterial feeding experiment. The results of this study provide insights to the targets of DON cytotoxicity and potential mitigation measures.

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RNASeq in C. elegans Following Manganese Exposure

Cited by 4 publications

References 42 publications

Preventing Parkinson’s Disease: An Environmental Agenda

Preventing Parkinson’s Disease: An Environmental Agenda

Measurement of the Effects of Metals on Taxis‐to‐Food Behavior in Caenorhabditis elegans

Transcriptome Analysis of C. elegans Reveals Novel Targets for DON Cytotoxicity

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