RNase P activity in the mitochondria of Saccharomyces cerevisiae depends on both mitochondrion and nucleus-encoded components.

Hollingsworth, Margaret J.; Martín, Nancy

doi:10.1128/mcb.6.4.1058

Cited by 116 publications

(64 citation statements)

References 25 publications

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“…The yeast mitochondrial RNase P, required for the 5' maturation of mitochondrial transfer RNAs (tRNAs), is one such enzyme. The nuclear genome provides the protein components necessary for biogenesis and function of this enzyme (2,3) and the mitochondrial genome provides an RNA thought to be the catalytic subunit (4,5).…”

Section: Introductionmentioning

confidence: 99%

Successful transformation of yeast mitochondria withRPM1: an approach forin vivostudies of mitochondrial RNase P RNA structure, function and biosynthesis

et al. 1995

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Mitochondrial RNase P RNA (Rpmlr) is coded by the RPM1 gene of mitochondrial DNA in many yeasts. As an initial step to developing a genetic approach to the structure and biogenesis of yeast mitochondrial RNase P, biolistic transformation has been used to introduce wild type and altered RPM1 genes into strains containing no mitochondrial DNA. The introduced wild type gene does support RNase P activity demonstrating that pre existing RNase P activity is not necessary for the biosynthesis of the enzyme. Mutations introduced into RPM1 in vitro result in reduced accumulation of mature tRNA and in an alteration of the processing of Rpml r in vivo.

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Section: Introductionmentioning

confidence: 99%

Successful transformation of yeast mitochondria withRPM1: an approach forin vivostudies of mitochondrial RNase P RNA structure, function and biosynthesis

et al. 1995

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“…Transcription begins at position Ϫ1566, with the A of the AUG initiation codon of COB defined as ϩ1. RNase P and tRNA 3Ј endonuclease process the initial transcript at the 5Ј and 3Ј ends of tRNA Glu , respectively (7,18), which releases tRNA Glu and COB precursor RNA with a 5Ј end extending to Ϫ1098. Further processing of COB precursor RNA generates the mature COB message with a 5Ј end at Ϫ955 or Ϫ954 (4).…”

mentioning

confidence: 99%

Genetic Evidence for Interaction between Cbp1 and Specific Nucleotides in the 5′ Untranslated Region of Mitochondrial Cytochrome b mRNA in Saccharomyces cerevisiae

Chen

Dieckmann

1997

Molecular and Cellular Biology

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The cytochrome b (COB) gene is encoded by the mitochondrial genome; however, its expression requires the participation of several nuclearly encoded protein factors. The yeast Cbp1 protein, which is encoded by the nuclear CBP1 gene, is required for the stabilization of COB mRNA. A previous deletion analysis identified an 11-nucleotide-long sequence within the 5 untranslated region of COB mRNA that is important for Cbp1-dependent COB mRNA stability. In the present study, site-directed mutagenesis experiments were carried out to define further the features of this cis element. The CCG sequence within this region was shown to be necessary for stability. A change in residue 533 of Cbp1 from aspartate to tyrosine suppresses the effects of a single-base change in the CCG element. This is strong genetic evidence that the nuclearly encoded Cbp1 protein recognizes and binds directly to the sequence containing CCG and thus protects COB mRNA from degradation.Mitochondrial biogenesis is a coordinated process that requires the function of both nuclear and mitochondrial gene products. For example, cytochrome b is an electron carrier located in the inner membrane of mitochondria along with other components of the respiratory chain and is encoded by the mitochondrial gene COB. Expression of COB requires participation of nuclearly encoded proteins. For example, Cbp2 (28, 42), Mrs1 (5,21,22), Mrs2 (20,43), and Nam2 (24, 26) are required for intron splicing; Cbp1 stabilizes COB mRNA (8, 12); and Cbs1 (29, 36) and Cbs2 (Cbp7) (29,34,35) are COB-specific translation factors. None of these nuclearly encoded factors is essential for growth of yeast on fermentable carbon sources (e.g., glucose), but strains with null mutations in the genes encoding these factors have a PET (petite) phenotype; they form smaller colonies than the wild-type strains on glucose media. PET mutants cannot grow on nonfermentable carbon sources (e.g., glycerol or ethanol), which require mitochondrial function for utilization.In yeast mitochondria, the genes for tRNA Glu and cytochrome b are cotranscribed as a unit (Fig. 1). Transcription begins at position Ϫ1566, with the A of the AUG initiation codon of COB defined as ϩ1. RNase P and tRNA 3Ј endonuclease process the initial transcript at the 5Ј and 3Ј ends of tRNA Glu , respectively (7, 18), which releases tRNA Glu and COB precursor RNA with a 5Ј end extending to Ϫ1098. Further processing of COB precursor RNA generates the mature COB message with a 5Ј end at Ϫ955 or Ϫ954 (4).Cbp1 was identified because it is required for the stabilization of COB mRNA (12). In cbp1 mutant strains, the level of tRNA Glu is wild type, COB precursor RNA is decreased to ϳ20% of the wild-type level, and the mature COB message is undetectable. Since inadequate apocytochrome b is produced, cbp1 mutant strains cannot respire. We previously used deletion analysis to locate a small region in the COB 5Ј UTR (untranslated region) which is essential for two Cbp1-dependent functions: positioning of the Ϫ955/Ϫ954 cleavage site and COB message ...

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“…The deviation in sequence and increased size of RPM2 suggests that the protein moiety could play a more substantial role in catalysis or molecular recognition. 4 A striking deviation from the canonical RNA-dependent RNase P is found in human mitochondria, where RNase P is PRORP. The localization of the nuclearencoded PRORP is currently unknown.…”

Section: The Varying Composition Of Rnase P Enzymesmentioning

confidence: 99%

RNase P enzymes

et al. 2013

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RNase P activity in the mitochondria of Saccharomyces cerevisiae depends on both mitochondrion and nucleus-encoded components.

Cited by 116 publications

References 25 publications

Successful transformation of yeast mitochondria withRPM1: an approach forin vivostudies of mitochondrial RNase P RNA structure, function and biosynthesis

Successful transformation of yeast mitochondria withRPM1: an approach forin vivostudies of mitochondrial RNase P RNA structure, function and biosynthesis

Genetic Evidence for Interaction between Cbp1 and Specific Nucleotides in the 5′ Untranslated Region of Mitochondrial Cytochrome b mRNA in Saccharomyces cerevisiae

RNase P enzymes

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