2004
DOI: 10.1007/s11103-004-0438-1
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RNase activity requires formation of disulfide bonds and is regulated by the redox state

Abstract: The activity of many RNases requires the formation of one or more disulfide bonds which can contribute to their stability. In this study, we show that RNase activity and, to a much lesser extent, nuclease activity, are redox regulated. Intracellular RNase activity was altered in vitro by changes in the glutathione redox state. Moreover, RNase activity was abolished following exposure to reducing agents such as beta-ME or DTT. Following reduction with glutathione (GSH), RNase activity could be fully reactivated… Show more

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Cited by 20 publications
(16 citation statements)
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“…In this study, we developed Direct-RT buffer that realizes cost-effective direct-lysate RT of crude plant, yeast and animal samples. DTT could reduce disulfide bonds required for the activity of RNase in lysates 24 . Because no loss of RNA in purification occurs through direct-lysate RT, this technique is beneficial also for gene expression analysis in small tissues from which it is difficult to purify enough amount of RNA for the experiments.…”
Section: Discussionmentioning
confidence: 99%
See 2 more Smart Citations
“…In this study, we developed Direct-RT buffer that realizes cost-effective direct-lysate RT of crude plant, yeast and animal samples. DTT could reduce disulfide bonds required for the activity of RNase in lysates 24 . Because no loss of RNA in purification occurs through direct-lysate RT, this technique is beneficial also for gene expression analysis in small tissues from which it is difficult to purify enough amount of RNA for the experiments.…”
Section: Discussionmentioning
confidence: 99%
“…In the previous research 24 , inactivation of RNase in an extract prepared from dark grown wheat required 0.5 mM DTT or more than 0.014% 2-mercaptoethanol (2ME) 24 .…”
Section: Reducing Regents Inhibit Rna Degradation In Plant Yeast Andmentioning
confidence: 99%
See 1 more Smart Citation
“…This strategy has previously been used to inhibit the degradation of RNA (2, 3), either by disulfide bridge reduction in RNases (4) or activation of endogenous RNase inhibitors (5). The new protocol was developed using neonatal rat ovaries, human granulosa cells (hGCs), and human corpus luteum (CL) by measuring the mRNA expression of the inhibin alpha subunit ( Inha) gene along with two housekeeping genes, the 60S ribosomal protein L32 (Rpl32) and glyceraldehyde 3-phosphate dehydrogenase (Gapdh), while measuring the enzymatic activities of phosphodiesterases (PDE), caspase-3, lactate dehydrogenase (LDH) and the immunoprecipitation of GAPDH and -actin.…”
mentioning
confidence: 99%
“…La actividad ADNasa y ARNasa de la nucleasa bifuncional de Arabidopsis BFN1 aumenta durante la senescencia foliar (Perez-Amador et al, 2000). En general, la actividad nucleasa in vitro es dependiente del estado redox y es inhibida por agentes quelantes (Chen et al, 2004).…”
Section: Degradación De áCidos Nucleicosunclassified