RNAi‐mediated silencing of ezrin gene reverses malignant behavior of human gastric cancer cell line SGC‐7901

Wang, Hong Jian; Zhu, Jin; Zhang, Qiang; Guo, Hua; Dai, Yun; Xiong, Xi

doi:10.1111/j.1751-2980.2009.00394.x

Cited by 21 publications

(13 citation statements)

References 28 publications

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“…In accordance with the tissue sample data indicating the correlation between miR-214 expression and LNM, we found that, compared to the moderately well-differentiated cell line MKN28, miR-214 expression was markedly less in the poorly differentiated cell lines MKN45 and BGC823, and especially the metastatic cell line SGC7901 [14] ( P <0.05).…”

Section: Resultssupporting

confidence: 89%

Clinicopathological Significance of MicroRNA-214 in Gastric Cancer and Its Effect on Cell Biological Behaviour

Wang

Shi

et al. 2014

PLoS ONE

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Accumulating evidence indicates that numerous microRNAs are involved in the tumorigenesis and progression of gastric cancer, while the clinical significance of microRNA-214 in gastric cancer is poorly understood and the exact role of microRNA-214 in gastric cancer remains obscure. In the present study, expression levels of microRNA-214 in 80 gastric carcinoma tissues, 18 nontumourous gastric tissues, and 4 types of gastric cancer cell lines were quantified by reverse transcription followed by real-time quantitative polymerase chain reaction (RT-qPCR), and the relationship between microRNA-214 expression and cliniopathological characteristics including prognosis was explored. To investigate the potential role of microRNA-214 in gastric cancer cell biological behaviour, we performed cell proliferation, apoptosis, migration and invasion assays in four gastric cancer cell lines and an immortalized gastric cell line in vitro. Our results showed that microRNA-214 was dramatically downregulated in gastric cancer tissues and gastric cancer cell lines, compared with nontumourous gastric tissues. Stepwise downregulation of microRNA-214 expression was observed among nontumourous gastric mucosa, nonmetastasis gastric cancer tissues, and metastasis gastric cancer tissues. The expression of microRNA-214 was significantly inversely correlated with lymph node metastasis and tumour size but had no correlation with the patient's prognosis. Ectopic expression of microRNA-214 could inhibit cell migration and invasion ability in SGC7901 and MKN45 gastric cancer cells. And knockdown of microRNA-214 significantly facilitated cell proliferation, migration and invasion in a cell-specific manner in MKN28, BGC823 and GES-1 cells. Colony stimulating factor 1 (CSF1) was identified as a target gene of microRNA-214. In summary, our data demonstrated that microRNA-214 is a promising novel biomarker for lymph node metastasis in patients with gastric cancer. And we identified that downregulation of microRNA-214 may regulate the proliferation, invasion and migration of gastric cancer cells by directly targeting CSF1.

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Section: Resultssupporting

confidence: 89%

Clinicopathological Significance of MicroRNA-214 in Gastric Cancer and Its Effect on Cell Biological Behaviour

Wang

Shi

et al. 2014

PLoS ONE

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“…There is a relationship between high expression of ezrin and metastatic potential of some carcinomas, including medulloblastoma [10], hepatocellular carcinoma [11], lung cancer [12], breast carcinoma [13], pancreatic adenocarcinoma [14], gastric cancer cell [15], and colorectal cancer [16]. We have also demonstrated that ezrin is overexpressed in a malignantly transformed esophageal epithelial cell line compared to an immortalized cell line [17].…”

mentioning

confidence: 63%

Potential transcriptional regulatory regions exist upstream of the human ezrin gene promoter in esophageal carcinoma cells

Gao¹,

Dai²,

Yin³

et al. 2011

ABBS

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We previously demonstrated that the region 287/1134 of the human ezrin gene (VIL2) exhibited promoter activity in human esophageal carcinoma EC109 cells, and a further upstream region 21324/2890 positively regulated transcription. In this study, to identify the transcriptional regulatory regions upstream of the VIL2 promoter, we cloned VIL2 21541/2706 segment containing the 21324/2890, and investigated its transcriptional regulatory properties via luciferase assays in transiently transfected cells. In EC109 cells, it was found that VIL2 21541/2706 possessed promoter and enhancer activities. We also localized transcriptional regulatory regions by fusing 5 0 -or 3 0 -deletion segments of VIL2 21541/2706 to a luciferase reporter. We found that there were three positive and one negative transcriptional regulatory regions within VIL2 21541/2706 in EC109 cells. When these regions were separately located upstream of the luciferase gene without promoter, or located upstream of the VIL2 promoter or SV40 promoter directing the luciferase gene, only VIL2 21297/21186 exhibited considerable promoter and enhancer activities, which were lower than those of 21541/2706. In addition, transient expression of Sp1 increased ezrin expression and the transcriptional activation of VIL2 21297/21186. Other three regions, although exhibiting significantly positive or negative transcriptional regulation in deletion experiments, showed a weaker or absent regulation. These data suggested that more than one region upstream of the VIL2 promoter participated in VIL2 transcription, and the VIL2 21297/21186, probably as a key transcriptional regulatory region, regulated VIL2 transcription in company with other potential regulatory regions.

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“…The inhibition of Ezrin expression clearly inhibits the migration and invasion of the human gastric cancer cell line, SGC-7901, and increases both cell adhesion and sensitivity to camptothecin-induced apoptosis [18]. Overexpression of Ezrin also promoted cell protrusion, microvillus formation, anchorage-independent growth, motility and invasion in the pancreatic cancer cell line, MiaPaCa-2.…”

Section: Discussionmentioning

confidence: 98%

Ezrin is Associated with Gastric Cancer Progression and Prognosis

Wang

Zhao

et al. 2011

Pathol. Oncol. Res.

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To investigat the clinical significance of Ezrin in the development and progression of gastric cancer. Immunohistochemistry was employed to analyze Ezrin expression in 436 clinicopathologically characterized gastric cancer cases. Ezrin protein levels were up-regulated in gastric cancer lesions compared with adjacent noncancerous tissues. Positive expression of Ezrin correlated with age, size of tumor, location of tumor, depth of invasion, vessel invasion, lymph node and distant metastasis and TNM stage. In stages I, II and III, the 5 year survival rate of patients with a high expression of Ezrin was significantly lower than those in patients with low expression. In stage IV, Ezrin expression did not correlate with the 5 year survival rate. Further multivariate analysis suggested that the depth of invasion, lymph node and distant metastasis, TNM stage, and up-regulation of Ezrin were independent prognostic indicators for the disease. Expression of Ezrin in gastric cancer is significantly associated with lymph node and distant metastasis, and poor prognosis. Ezrin protein could be useful markers to predict tumor progression and prognosis.

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RNAi‐mediated silencing of ezrin gene reverses malignant behavior of human gastric cancer cell line SGC‐7901

Cited by 21 publications

References 28 publications

Clinicopathological Significance of MicroRNA-214 in Gastric Cancer and Its Effect on Cell Biological Behaviour

Clinicopathological Significance of MicroRNA-214 in Gastric Cancer and Its Effect on Cell Biological Behaviour

Potential transcriptional regulatory regions exist upstream of the human ezrin gene promoter in esophageal carcinoma cells

Ezrin is Associated with Gastric Cancer Progression and Prognosis

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