RNAi mediated knock down of 3β-hydroxysteroid dehydrogenases in Digitalis lanata shoot cultures

Overexpression of 3β-hydroxysteroid dehydrogenases in Digitalis lanata shoot cultures

“…Similar results were reported for OE lines, expressing progesterone 5β-reductase, another gene involved in cardenolide biosynthesis 4 . We conclude that RNAi-mediated knockdown is a more effective approach to demonstrate participation of certain enzymes in biosynthetic pathways 2 5 .…”