RNAi-Mediated Inhibition of Apoptosis Fails to Prevent Cationic Nanoparticle-Induced Cell Death in Cultured Cells

Bexiga, Mariana G.; Kelly, Ciara; Dawson, Kenneth A.; Simpson, Jeremy C.

doi:10.2217/nnm.13.151

Cited by 14 publications

(21 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Flow cytometric analysis of phosphatydilserine exposure measured by AnnexinV-FITC and Propidium Iodide (PI) uptake revealed a population of early apoptotic cells (AnnexinV positive and PI negative), following exposure of A549 cells, while only necrotic cells (AnnexinV positive and PI positive) could be observed following exposure of RAW264.7 cells, consistent with the previous findings showing that PS-NH 2 NPs induced apoptosis in A549 cells [43], [45], and further supporting the hypothesis that RAW264.7 cells underwent necrosis as opposed to apoptosis (Figure S4 in File S1). …”

Section: Resultssupporting

confidence: 89%

High Content Analysis Provides Mechanistic Insights on the Pathways of Toxicity Induced by Amine-Modified Polystyrene Nanoparticles

et al. 2014

View full text Add to dashboard Cite

The fast-paced development of nanotechnology needs the support of effective safety testing. We have developed a screening platform measuring simultaneously several cellular parameters for exposure to various concentrations of nanoparticles (NPs). Cell lines representative of different organ cell types, including lung, endothelium, liver, kidney, macrophages, glia, and neuronal cells were exposed to 50 nm amine-modified polystyrene (PS-NH2) NPs previously reported to induce apoptosis and to 50 nm sulphonated and carboxyl-modified polystyrene NPs that were reported to be silent. All cell lines apart from Raw 264.7 executed apoptosis in response to PS-NH2 NPs, showing specific sequences of EC50 thresholds; lysosomal acidification was the most sensitive parameter. Loss of mitochondrial membrane potential and plasma membrane integrity measured by High Content Analysis resulted comparably sensitive to the equivalent OECD-recommended assays, allowing increased output. Analysis of the acidic compartments revealed good cerrelation between size/fluorescence intensity and dose of PS-NH2 NPs applied; moreover steatosis and phospholipidosis were observed, consistent with the lysosomal alterations revealed by Lysotracker green; similar responses were observed when comparing astrocytoma cells with primary astrocytes. We have established a platform providing mechanistic insights on the response to exposure to nanoparticles. Such platform holds great potential for in vitro screening of nanomaterials in highthroughput format.

show abstract

Section: Resultssupporting

confidence: 89%

High Content Analysis Provides Mechanistic Insights on the Pathways of Toxicity Induced by Amine-Modified Polystyrene Nanoparticles

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Despite data on environmental concentrations of similar particles in sea water are not available, these concentrations may be far above real exposure conditions. PS-NH 2 caused apoptosis in 1321N1 human cells at 50 mg/ml (Bexiga et al, 2014), but in our previous study (Della Torre et al, 2014a) we observed induction of an apoptotic pathway in sea urchin developing embryos already at 3 mg/ml, thus raising concern regarding the impact of lower PS NPs concentrations in marine organisms.…”

Section: Physico-chemical Characterization Of Ps-cooh and Ps-nh 2 Npsmentioning

confidence: 67%

Nano-sized polystyrene affects feeding, behavior and physiology of brine shrimp Artemia franciscana larvae

Bergami

Bocci

Vannuccini

et al. 2016

Ecotoxicology and Environmental Safety

295

164

View full text Add to dashboard Cite

“…Concentration up to 50 μg mL −1 was shown to induce apoptosis in 1321N1 human cells treated for 24 h with PS-NH 2 NPs. 43 2.2. Sea Urchin Maintenance, Gametes Collection and PS NPs Exposure.…”

Section: Methodsmentioning

confidence: 99%

Accumulation and Embryotoxicity of Polystyrene Nanoparticles at Early Stage of Development of Sea Urchin Embryos Paracentrotus lividus

Torre

Bergami

Salvati

et al. 2014

Environ. Sci. Technol.

528

216

View full text Add to dashboard Cite

Nanoplastic debris, resulted from runoff and weathering breakdown of macro- and microplastics, represents an emerging concern for marine ecosystems. The aim of the present study was to investigate disposition and toxicity of polystyrene nanoparticles (NPs) in early development of sea urchin embryos (Paracentrotus lividus). NPs with two different surface charges where chosen, carboxylated (PS-COOH) and amine (PS-NH2) polystyrene, the latter being a less common variant, known to induce cell death in several in vitro cell systems. NPs stability in natural seawater (NSW) was measured while disposition and embryotoxicity were monitored within 48 h of postfertilization (hpf). Modulation of genes involved in cellular stress response (cas8, 14-3-3ε, p-38 MAPK, Abcb1, Abcc5) was investigated. PS-COOH forms microaggregates (PDI > 0.4) in NSW, whereas PS-NH2 results are better dispersed (89 ± 2 nm) initially, though they also aggregated partially with time. Their respectively anionic and cationic nature was confirmed by ζ-potential measurements. No embryotoxicity was observed for PS-COOH up to 50 μg mL(-1) whereas PS-NH2 caused severe developmental defects (EC50 3.85 μg mL(-1) 24 hpf and EC50 2.61 μg mL(-1) 48 hpf). PS-COOH accumulated inside embryo's digestive tract while PS-NH2 were more dispersed. Abcb1 gene resulted up-regulated at 48 hpf by PS-COOH whereas PS-NH2 induced cas8 gene at 24 hpf, suggesting an apoptotic pathway. In line with the results obtained with the same PS NPs in several human cell lines, also in sea urchin embryos, differences in surface charges and aggregation in seawater strongly affect their embryotoxicity.

show abstract

RNAi-Mediated Inhibition of Apoptosis Fails to Prevent Cationic Nanoparticle-Induced Cell Death in Cultured Cells

Cited by 14 publications

References 40 publications

High Content Analysis Provides Mechanistic Insights on the Pathways of Toxicity Induced by Amine-Modified Polystyrene Nanoparticles

High Content Analysis Provides Mechanistic Insights on the Pathways of Toxicity Induced by Amine-Modified Polystyrene Nanoparticles

Nano-sized polystyrene affects feeding, behavior and physiology of brine shrimp Artemia franciscana larvae

Accumulation and Embryotoxicity of Polystyrene Nanoparticles at Early Stage of Development of Sea Urchin Embryos Paracentrotus lividus

Contact Info

Product

Resources

About