RNAi-mediated down-regulation of ITPK-2 enhanced inorganic phosphorus and minerals in the transgenic rice

Sengupta, Suman; Bhattacharya, Sananda; Karmakar, Aritra; Ghosh, Subhrajyoti; Sarkar, Sailendra Nath; Gangopadhyay, Gaurab; Datta, Karabi; Datta, Swapan K.

doi:10.1007/s12038-021-00154-6

Cited by 4 publications

(2 citation statements)

References 34 publications

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“…HQ123045.1) designed by Primer Premier 5 are listed in Table 1. After the data were normalised, variations in gene expression data among different samples were expressed as 2 -ΔΔCT (Sengupta et al 2021). The strain with the lowest ACS expression level was selected for the subsequent analysis.…”

Section: Methodsmentioning

confidence: 99%

Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in Monascus

Lin¹,

Zhang²,

Shao³

et al. 2022

Czech J. Food Sci.

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Acetyl-coenzyme A (CoA) is a key metabolite produced by the acetyl-CoA synthetase (ACS) gene in energy metabolism and biosynthetic pathways. ACS is speculated to be the branching site of monacolin K (MK) and citrinin production and related to the metabolite production of Monascus. In this study, the ACS expression was inhibited by ribonucleic acid interference (RNAi). T7 was selected for a follow-up analysis of the lowest ACS expression, which was 0.401 times higher than that of the wild-type strain. The effects on the colony morphology of Monascus were determined. The morphological characteristics of mycelia and spores were observed under a scanning electron microscope. The contents of secondary metabolites, namely, MK and citrinin, were determined through high performance liquid chromatography (HPLC). Colour values were measured with a spectrophotometer. Results showed that the low ACS expression could inhibit the growth of Monascus colonies and the hypha and affect the formation and morphology of Monascus M1 spores. It could also inhibit the production of the main secondary metabolites, namely, MK, citrinin, and pigment.

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Section: Methodsmentioning

confidence: 99%

Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in Monascus

Lin¹,

Zhang²,

Shao³

et al. 2022

Czech J. Food Sci.

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show abstract

“…The three targeted enzymes were myo-inositol-3-phosphate synthase, inositol-1,3,4,5,6-pentakisphosphate 2-kinase (IPK1), and inositol triphosphate kinases (ITPK) homolog (OsITP/6K-1), resulting in a 1.3-1.8-fold increase of Fe in milled rice (Ali et al, 2013a(Ali et al, , 2013bKarmakar et al, 2020). In addition, RNAi-mediated downregulation of ITPK-2 has resulted in enhanced inorganic P along with Zn 2+ and Fe 2+ in transgenic rice (Sengupta et al, 2021). Increased Fe translocation in rice endosperm was also achieved by the overexpression of Fe transporter gene OsYSL2 (Masuda et al, 2013).…”

Section: Transgenic Approachesmentioning

confidence: 99%

Mineral nutrients in plants under changing environments: A road to future food and nutrition security

et al. 2023

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Plant nutrition is an important aspect that contributes significantly to sustainable agriculture, whereas minerals enrichment in edible source implies global human health; hence, both strategies need to be bridged to ensure “One Health” strategies. Abiotic stress‐induced nutritional imbalance impairs plant growth. In this context, we discuss the molecular mechanisms related to the readjustment of nutrient pools for sustained plant growth under harsh conditions, and channeling the minerals to edible source (seeds) to address future nutritional security. This review particularly highlights interventions on (i) the physiological and molecular responses of mineral nutrients in crop plants under stressful environments; (ii) the deployment of breeding and biotechnological strategies for the optimization of nutrient acquisition, their transport, and distribution in plants under changing environments. Furthermore, the present review also infers the recent advancements in breeding and biotechnology‐based biofortification approaches for nutrient enhancement in crop plants to optimize yield and grain mineral concentrations under control and stress‐prone environments to address food and nutritional security.

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An Overview of Targeted Genome Editing Strategies for Reducing the Biosynthesis of Phytic Acid: an Anti-nutrient in Crop Plants

et al. 2023

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RNAi-mediated down-regulation of ITPK-2 enhanced inorganic phosphorus and minerals in the transgenic rice

Cited by 4 publications

References 34 publications

Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in Monascus

Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in Monascus

Mineral nutrients in plants under changing environments: A road to future food and nutrition security

An Overview of Targeted Genome Editing Strategies for Reducing the Biosynthesis of Phytic Acid: an Anti-nutrient in Crop Plants

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