RNAi induced silencing of pathogenicity genes of Fusarium spp. for vascular wilt management in tomato

Shanmugam, V.; Sharma, Vandana; Bharti, Poonam; Jyoti, Poonam; Yadav, Sudesh Kumar; Aggarwal, Rashmi; Jain, Shekhar

doi:10.1007/s13213-017-1265-3

Cited by 12 publications

(4 citation statements)

References 22 publications

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“…Phenotypic analysis showed reduced virulence and altered physiological characteristics after the silencing of both genes. Such target genes could be useful to study and develop dsRNA fungicide to manage Fusarium wilt (Shanmugam et al 2017 ).…”

Section: Dsrna Fungi- and Pesticide For Forest Insect Pests And Funga...mentioning

confidence: 99%

Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities

Singewar¹,

Fladung²

2023

Funct Integr Genomics

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Climate change alters the seasonal synchronization between plants and respective pests plus pathogens. The geographical infiltration helps to shift their hosts, resulting in novel outbreaks that damage forests and ecology. Traditional management schemes are unable to control such outbreaks, therefore unconventional and competitive governance is needed to manage forest pests and pathogens. RNA interference (RNAi) mediated double-stranded RNA (dsRNA) treatment method can be implemented to protect forest trees. Exogenous dsRNA triggers the RNAi-mediated gene silencing of a vital gene, and suspends protein production, resulting in the death of targeted pathogens and pests. The dsRNA treatment method is successful for many crop insects and fungi, however, studies of dsRNA against forest pests and pathogens are depleting. Pesticides and fungicides based on dsRNA could be used to combat pathogens that caused outbreaks in different parts of the world. Although the dsRNA has proved its potential, the crucial dilemma and risks including species-specific gene selection, and dsRNA delivery methods cannot be overlooked. Here, we summarized the major fungi pathogens and insect pests that have caused outbreaks, their genomic information, and studies on dsRNA fungi-and pesticides. Current challenges and opportunities in dsRNA target decision, delivery using nanoparticles, direct applications, and a new method using mycorrhiza for forest tree protection are discussed. The importance of affordable next-generation sequencing to minimize the impact on non-target species is discussed. We suggest that collaborative research among forest genomics and pathology institutes could develop necessary dsRNA strategies to protect forest tree species.

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Section: Dsrna Fungi- and Pesticide For Forest Insect Pests And Funga...mentioning

confidence: 99%

Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities

Singewar¹,

Fladung²

2023

Funct Integr Genomics

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“…( Šečić and Kogel, 2021 ). Therefore, further investigation on the target genes of F. fujikuroi must be encouraged to be able to ensure a comprehensive IDM ( Koch et al, 2016 ; Shanmugam et al, 2017 ).…”

Section: Conclusion and Way Forwardmentioning

confidence: 99%

Past and Future Epidemiological Perspectives and Integrated Management of Rice Bakanae in Korea

et al. 2023

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In the past, rice bakanae was considered an endemic disease that did not cause significant losses in Korea; however, the disease has recently become a serious threat due to climate change, changes in farming practices, and the emergence of fungicide-resistant strains. Since the bakanae outbreak in 2006, its incidence has gradually decreased due to the application of effective control measures such as hot water immersion methods and seed disinfectants. However, in 2013, a marked increase in bakanae incidence was observed, causing problems for rice farmers. Therefore, in this review, we present the potential risks from climate change based on an epidemiological understanding of the pathogen, host plant, and environment, which are the key elements influencing the incidence of bakanae. In addition, disease management options to reduce the disease pressure of bakanae below the economic threshold level are investigated, with a specific focus on resistant varieties, as well as chemical, biological, cultural, and physical control methods. Lastly, as more effective countermeasures to bakanae, we propose an integrated disease management option that combines different control methods, including advanced imaging technologies such as remote sensing. In this review, we revisit and examine bakanae, a traditional seed-borne fungal disease that has not gained considerable attention in the agricultural history of Korea. Based on the understanding of the present significance and anticipated risks of the disease, the findings of this study are expected to provide useful information for the establishment of an effective response strategy to bakanae in the era of climate change.

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“…However, silencing of the Hog1 gene in F. graminearum showed significantly reduced conidiation [ 207 ]. Furthermore, the silencing of FOW2 and chsV (class V chitin synthase) in F. oxysporum and F. solani showed reduced mycelial growth and sporulation, which confirmed their involvement in pathogenicity [ 208 ]. In F. oxysporum f.sp cubense , the SGE1 gene ( Six gene expression 1 ) is involved in pathogenicity and virulence.…”

Section: Artificial Control Of Sporulation In Fusariummentioning

confidence: 99%

Strategies for Controlling the Sporulation in Fusarium spp.

Ajmal

Hussain

Ali

et al. 2022

JoF

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Fusarium species are the most destructive phytopathogenic and toxin-producing fungi, causing serious diseases in almost all economically important plants. Sporulation is an essential part of the life cycle of Fusarium. Fusarium most frequently produces three different types of asexual spores, i.e., macroconidia, chlamydospores, and microconidia. It also produces meiotic spores, but fewer than 20% of Fusaria have a known sexual cycle. Therefore, the asexual spores of the Fusarium species play an important role in their propagation and infection. This review places special emphasis on current developments in artificial anti-sporulation techniques as well as features of Fusarium’s asexual sporulation regulation, such as temperature, light, pH, host tissue, and nutrients. This description of sporulation regulation aspects and artificial anti-sporulation strategies will help to shed light on the ways to effectively control Fusarium diseases by inhibiting the production of spores, which eventually improves the production of food plants.

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RNAi induced silencing of pathogenicity genes of Fusarium spp. for vascular wilt management in tomato

Cited by 12 publications

References 22 publications

Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities

Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities

Past and Future Epidemiological Perspectives and Integrated Management of Rice Bakanae in Korea

Strategies for Controlling the Sporulation in Fusarium spp.

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