RNAi-induced knockdown of white gene in the southern green stink bug (Nezara viridula L.)

Abstract

The southern green stink bug (SGSB) Nezara viridula L. is one of the most common stink bug species in the United States and can cause significant yield loss in a variety of crops. A suitable marker for the assessment of gene-editing tools in SGSB has yet to be characterized. The white gene, first documented in Drosophila, has been a useful target to assess the efficiency of introduced mutations in many species as it controls pigmentation processes and mutants display readily identifiable phenotypes. In this study we used the RNAi technique to investigate functions and phenotypes associated with the white ortholog in the SGSB and to validate white as a marker for genetic transformation in this species. This study revealed that white may be a suitable marker for germline transformation in the SGSB as white transcript knockdown was not lethal, did not impair embryo development and provided a distinguishable phenotype. Our results demonstrated that the white ortholog in SGSB is involved in the pathway for ommochrome synthesis and suggested additional functions of this gene such as in the integument composition, management of hemolymph compounds and riboflavin mobilization.

Characterization of Ionotropic Receptor Gene EonuIR25a in the Tea Green Leafhopper, Empoasca onukii Matsuda

“…In recent years, RNAi has attracted much interest as a pest control tool [ 54 ], and the stability of dsRNA and the ultimate effectiveness and specificity of RNAi are worth considering [ 55 ]. Currently, the most commonly applied dsRNA delivery methods in insects are microinjection and feeding [ 56 , 57 , 58 , 59 ]. It is noteworthy that most microinjections are time-consuming and require a variety of equipment from in-house manufactured devices to complex microprocessor-controlled syringes, while microinjections are obviously limited to the laboratory [ 57 ].…”

Current Scenario of Exogenously Induced RNAi for Lepidopteran Agricultural Pest Control: From dsRNA Design to Topical Application

“…In the case of the fall armyworm (Spodoptera frugiperda; Lepidoptera), the basic transcription levels of these core elements of RNAi machinery are similar to those observed in western corn rootworm (Diabrotica virgifera; Coleoptera) and southern green stink bug (Nezara viridula; Hemiptera) [39]. All three taxa are considered highly efficient species on which to employ RNAi strategies [40][41][42], from phylogenetic orders whose response to dsRNAi varies.…”

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“…была на уровне некоторых коммерческих гербицидов (Da Cruz-Silva et al, 2021). Из листьев осота полевого (Sonchus arvensis L.) был выделены сесквитерпеноиды, которые проявили селективную фитотоксическую активность против щирицы запрокинутой (A. retroflexus) и мари белой (Chenopodium album L.) на уровне триасульфурона, не действуя при этом на пшеницу (Wu et al, 2022).…”

“…4.5. Клопы РНКи у клопов (Heteroptera) изучали как на растительноядных представителях семейств Miridae (Walker, Allen al., 2011;Allen, Walker, 2012;Liu et al, 2019), Lygaeidae (Bewick et al, 2019), Pyrrhocoridae (Kostal, Tollarova-Borovanska, 2009;Kotwica-Rolinska et al, 2017), Alydidae (Ikeno et al, 2014), Pentatomidae (Sugahara et al, 2016;Mogilicherla et al, 2018;Castellanos et al, 2019;Souza et al, 2022;Finetti et al, 2023) и Scutelleridae (Amiri et al, 2016;Amiri, Bandani, 2020), многие из которых являются злостными вредителями сельскохозяйственных культур, так и на кровососущих видах -переносчиках опасных болезней человека и животных из семейств Reduviidae (Paim et al, 2013;Salcedo-Porras et al, 2019;Finetti et al, 2023) и Cimicidae (Basnet, Kamble, 2018). В большинстве работ с клопами доставка дцРНК осуществлялась инъекцией, поверхностным нанесением или с кормом.…”