RNA2 of Soil-borne cereal mosaic virus is detectable in plants of winter wheat grown from infected seeds

Budge, Giles E.; Loram, J.; Donovan, Gregory R.; Boonham, Neil

doi:10.1007/s10658-007-9194-9

Cited by 8 publications

(5 citation statements)

References 11 publications

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“…Evolutionary distances were computed using the Maximum Composite Likelihood Method. Also, there is evidence that SBCMV may be seedbome in wheat (7), and also spread through surface contamination of seeds with viruliferous P. graminis resting spores so soil-borne viruses are likely to be introduced in this way to new regions. The phylogenetic tree was generated using MEGA 5 (32).…”

Section: Discussionmentioning

confidence: 99%

Polymyxa graminis Isolates from Australia: Identification in Wheat Roots and Soil, Molecular Characterization, and Wide Genetic Diversity

2014

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A ustralia; H. Luo, C rop Protection Branch, D epartm ent o f A griculture and Food W est ern A ustralia, Perth, WA 6983, A ustralia; and R. A. C. Jones, Crop Protection Branch, D epartm ent of A griculture and Food Western A ustralia, Abstract Cox, B. A., Luo, H., and Jones, R. A. C. 2014. Polymyxa graminis isolates from Australia: Identification in wheat roots and soil, molecular charac terization, and wide genetic diversity. Plant Dis. 98:1567-1575.Polymyxa graminis is an obligate parasite of roots and an important vector of viruses that damage cereal crops in different parts of the world. In 2011 and 2012, P. graminis was identified infecting 11 wheat root samples from three widely dispersed locations in southwest Aus tralia. Its presence was detected by polymerase chain reaction (PCR) and confirmed by DNA sequencing of the transcribed regions of its ribosomal RNA genes (rDNA) and observing sporosori of character istic morphology and size in stained wheat roots. Also, when soil sam ples were collected from two locations where P. graminis was found and wheat bait plants grown in them, P. graminis was detected in their roots by PCR. Ribosomal DNA sequences of six southwest Australian isolates were obtained from wheat roots, and one northeast Australian isolate from barley roots. When these seven P. graminis sequences were compared with others from GenBank by phylogenetic analysis, three southwest Australian isolates were classified as P graminis f. sp. temperata (ribotypes la and lb), and three as f. sp. tepida (ribotypes Ila and lib). P graminis f. sp. temperata and tepida both occur in Corresponding author: R. A. C. Jones,

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Section: Discussionmentioning

confidence: 99%

Polymyxa graminis Isolates from Australia: Identification in Wheat Roots and Soil, Molecular Characterization, and Wide Genetic Diversity

2014

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“…We also aimed at examining the response of chosen winter wheat and winter triticale cultivars to the infection. In the epidemiology of SBCMV, the seed transmission capacity of the virus may play an important role (Garbaczewska et al 1997;Jeżewska 2006;Budge et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Studies on Cereal Soil-Borne Viruses in Poland

Jeżewska¹,

Trzmiel²

2010

Journal of Plant Protection Research

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Studies on Cereal Soil-Borne Viruses in PolandFour soil-borne cereal viruses have been identified in Poland, so far:Soil-borne cereal mosaic virus(SBCMV),Wheat spindle streak mosaic virus(WSSMV),Barley yellow mosaic virus(BaYMV) andBarley mild mosaic virus(BaMMV). SBCMV was identified in 1993 as a dangerous pathogen of winter cereals and became the object of special interest. Studies on the virus included its biological and molecular characterization, and investigations of the response of winter wheat and winter triticale cultivars on the SBCMV infection. Results of preliminary experiments aiming at the evaluation of the response of winter barley cultivars on barley yellow mosaic viruses were also presented.

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“…It was concluded that the virus isolate was successfully transmitted via seeds in spite of previous certainty that SBCMV could not be transmitted this way. Additional data concerning the possibility of seed transmission of SBCMV was provided in subsequent years (Jeżewska 2006;Budge et al 2008). However the doubts of some researchers were not definitely dispelled because only RNA2 of SBCMV was detectable in winter wheat plants grown from infected seeds.…”

Section: Resultsmentioning

confidence: 99%

Detection of RNA1 and RNA2 of Soil-borne wheat mosaic virus in winter wheat grown from infected seeds

Jeżewska¹,

Trzmiel²,

Zarzyńska‐Nowak³

2016

Journal of Plant Protection Research

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A Polish isolate of Soil-borne wheat mosaic virus (SBWMV-Pol1) was characterized by limited pathogenicity and a low concentration of virus particles in infected plant tissues. The aim of this research was to consider the possibility of seed-transmission dissemination of the virus. Seeds of winter wheat cv. Muszelka served as material for the studies. Two methods were involved in the diagnostics of seedlings grown from potentially infected seeds: enzyme-linked immunosorbent assay (ELISA), as the screening assay and immuno-capture-reverse transcription-polymerase chain reaction (IC-RT-PCR) for molecular confirmation of the infection.RNA1 and RNA2 of SBWMV-Pol1 were detected in 6 out of 1,410 plants submitted to diagnostic procedures. The possibility of seed transmission of SBWMV-Pol1 was discussed.

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RNA2 of Soil-borne cereal mosaic virus is detectable in plants of winter wheat grown from infected seeds

Cited by 8 publications

References 11 publications

Polymyxa graminis Isolates from Australia: Identification in Wheat Roots and Soil, Molecular Characterization, and Wide Genetic Diversity

Polymyxa graminis Isolates from Australia: Identification in Wheat Roots and Soil, Molecular Characterization, and Wide Genetic Diversity

Studies on Cereal Soil-Borne Viruses in Poland

Detection of RNA1 and RNA2 of Soil-borne wheat mosaic virus in winter wheat grown from infected seeds

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