RNA Tumor Virus Antigens

Oroszlan, Stephen; Gilden, Raymond V.

doi:10.1111/j.1365-3083.1978.tb03875.x

Cited by 9 publications

(3 citation statements)

References 72 publications

(57 reference statements)

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“…Further, a study (13) of 1540 human cord blood sera revealed the presence of RD114 p30-reactive antibodies in 118 (7.7%) sera. Antibodies to synthetic peptides, which were based on MuLV p30 amino acid sequences or sequences of env genes, have been found to be reactive with the native structural proteins of murine retroviruses (14). In the present study we used antibodies to an undecapeptide, which has partial sequence homology to MuLV and BaEV p30 proteins.…”

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confidence: 99%

Human placental syncytiotrophoblastic Mr 75,000 polypeptide defined by antibodies to a synthetic peptide based on a cloned human endogenous retroviral DNA sequence.

Suni¹,

Närvänen²,

Wahlström³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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Antibodies to a synthetic undecapeptide (NH2-Cys-Glu-Asn-Pro-Ser-Gln-Phe-Tyr-Glu-Arg-Leu-COOH), the sequence (except cysteine) of which was deduced from a previously reported cloned human retroviral gag-generelated DNA sequence ern-1, were raised in rabbits. In immunohistochemical staining these antibodies reacted with normal human first-trimester placentas and with blighted ova and benign and malignant trophoblastic tumors (hydatidiform and destructive moles, choriocarcinomas) but not with any other normal embryonic or adult tissues tested. In all tissues the reactivity was mainly confined to cells with trophoblastic morphology. In immunoblotting the antibody detected an Mr 75,000 polypeptide in syncytiotrophoblasts isolated from first-trimester placentas and in three different lines of cultured choriocarcinoma cells. The undecapeptide blocked the reactivity of the antibody.In a previous study (13) using the feline endogenous retrovirus RD114 and antibodies to its major structural protein p30 as reagents, we demonstrated p30-related antigen in human placental syncytiotrophoblasts. Further, a study (13) of 1540 human cord blood sera revealed the presence of RD114 p30-reactive antibodies in 118 (7.7%) sera. Antibodies to synthetic peptides, which were based on MuLV p30 amino acid sequences or sequences of env genes, have been found to be reactive with the native structural proteins of murine retroviruses (14). In the present study we used antibodies to an undecapeptide, which has partial sequence homology to MuLV and BaEV p30 proteins. The undecapeptide sequence was inferred from the nucleotide sequence of the human proviral locus, erv-1 (7). This antibody detects a polypeptide antigen in syncytiotrophoblastic cells of human placentas both in vivo and in culture. Type C endogenous retroviruses are widespread in birds and mammals. They have also been recovered from several primates, including the Old World monkeys, baboon, stump-tail macaque, rhesus, and colobus, and the New World owl monkeys. The major source for isolation of endogenous retrovirus from baboons is the placenta (see ref. 1 for review). In humans, particles resembling type C or type D retroviruses have been seen budding from the syncytiotrophoblast layer of placentas (2, 3) and from cultured teratocarcinoma cells (4). In addition, small retrovirus-like particles have been seen in some human oocytes (5). Human endogenous retroviruses have not been isolated in an infectious form and producer cell lines have not been established.Evidence has been presented that human DNA contains retrovirus-related nucleotide sequences. Nucleic acid reassociation studies first revealed that humans and other primates contain multiple baboon endogenous virus (BaEV)-related sequences in their chromosomal DNA (6). A defective, endogenous provirus was recently isolated from a human recombinant DNA library by using as probe an endogenous chimpanzee retroviral pol fragment highly related to that of BaEV (7). This genome, termed HC-20 (or endogenous retrovirus-1; erv-1...

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confidence: 99%

Human placental syncytiotrophoblastic Mr 75,000 polypeptide defined by antibodies to a synthetic peptide based on a cloned human endogenous retroviral DNA sequence.

Suni¹,

Närvänen²,

Wahlström³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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“…This apparent loss of an interspecies determinant (9) may be due to the modification within that determinant of an amino acid other than lysine. Recent studies with a synthetic undecapeptide (29,32), representing the N-terminal conserved region of mammalian p3Os (27), and with an octacosapeptide (33), corresponding to the entire N-terminal segment of gibbon ape leukemia virus which contains this common region, indicate that the common hydroxyamino acids may be involved in one of the interspecies antigenic sites (D. L. Fine and S. Oroszlan, manuscript in preparation).…”

Section: Competition Assays With "Li-labeled R-mulv P30mentioning

confidence: 99%

“…The mammalian type C viral proteins gp70 and p30 have been shown to contain type-specific, group-specific, and interspecies-specific determinants (13,29,37). We previously investigated the relationship among these determinants in p30 by various immunochemical methods (6-9).…”

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Effect of Chemical Modification and Fragmentation on Antigenic Determinants of Internal Protein p30 and Surface Glycoprotein gp70 of Type C Retroviruses

Versteegen

Oroszlan

1980

J Virol

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The effects of protein modification on the antigenic determinants of p30 and gp7O of type C retroviruses were investigated by using solid-phase competition radioimmunoassays. Proteins were modified by reduction with 2-mercaptoethanol and subsequent carboxymethylation of SH groups with iodoacetamide or by amidination of a and e amino groups with methylacetimidate. The type-specific determinants of gp7O were found to be conformational in nature, as they were destroyed by these chemical modifications. Groupand interspecies-specific determinants of gp7O antigens, however, appear to be sequential and do not involve residues susceptible to these chemical reagents. Conformation-dependent typespecific determinants of p30 were affected only by methylacetimidate. Group-and interspecies-specific determinants of p30 are similar to those of gp7O in that they also appear to be sequential antigenic sites. Therefore, the broadly reactive groupand interspecies-specific determinants of gp7O and p30 can be followed into small peptides. Accordingly, a cyanogen bromide cleavage fragment derived from the carboxyl-terminal one-third of Rauscher leukemia virus p30 was found to carry group-specific determinants but no detectable interspecies-specific determinants. In contrast, a peptide obtained by limited trypsin cleavage of p30, which was derived from the NH2-terminal region of the protein, contained at least one of the interspecies determinants shared with feline leukemia virus p27.

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Amino Acid Sequences of Plant and Animal Viral Proteins

Oroszlan

Gilden

1979

Comprehensive Virology Volume 13: Structure and Assembly

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RNA Tumor Virus Antigens

Cited by 9 publications

References 72 publications

Human placental syncytiotrophoblastic Mr 75,000 polypeptide defined by antibodies to a synthetic peptide based on a cloned human endogenous retroviral DNA sequence.

Human placental syncytiotrophoblastic Mr 75,000 polypeptide defined by antibodies to a synthetic peptide based on a cloned human endogenous retroviral DNA sequence.

Effect of Chemical Modification and Fragmentation on Antigenic Determinants of Internal Protein p30 and Surface Glycoprotein gp70 of Type C Retroviruses

Amino Acid Sequences of Plant and Animal Viral Proteins

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