RNA Synthesis Initiates
            <i>In Vitro</i>
            Conversion of M13 DNA to Its Replicative Form

Wickner, William; Brutlag, Douglas L.; Schekman, Randy; Kornberg, Arthur

doi:10.1073/pnas.69.4.965

Cited by 288 publications

(96 citation statements)

References 9 publications

(1 reference statement)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recently, it was shown that in vivo (7) and in soluble extracts of E. coli (8), the conversion of M13 ss DNA into RF is inhibited by rifampicin. When cells of HfrH 165/70 were treated with 200 /g/ml of rifampicin for 10 min and then infected with 32P-labeled M13, the antibiotic quantitatively inhibited the conversion of ss DNA to RF at both 34 and 410.…”

mentioning

confidence: 99%

Replication of Bacteriophage M13: Specificity of the Escherichia coli dna B Function for Replication of Double-Stranded M13 DNA

Olsen¹,

Staudenbauer²,

Hofschneider³

1972

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Infection of the temperature-sensitive E. eli mutant HfrH 165/70 (dnaB) with the filamentous ..1-Stranded DNA phage M13 is abortive at the restrictive temperature. Upon infection at 410, singlestranded phage DNA penetrates the cell and is converted in a rifampicin-sensitive step to the double-stranded replicative form (RF). The parental RF attaches to the cell membrane, but subsequent replication of the RF is blocked. It is concluded that in M13 infection semiconservative RF replication of a double strand to a double strand, in contrast to single-stranded DNA synthesis, depends specifically on the dnaB function.In strains of E. coli containing a temperature-sensitive mutation in the dnaB region, infection by the single-stranded DNA phage M13 is inhibited at the restrictive temperature (1). However, synthesis of phage single-stranded (ss) DNA and liberation of mature phage particles continues after cells that are actively producing phage are shifted to the higher temperature (1)t. This result implies that there is an earlier step in the replication process of M13 that depends on the dnaB function. Since a precise localization of the block in M13 replication may contribute information both to the replicative process of single-stranded DNA phages and to the biochemical lesion in the dnaB mutant, abortive M13 infection at the restrictive temperature was studied in more detail. At the restrictive temperature, M13 ss DNA penetrates the cell and is readily converted to RF. In contrast, neither the parental nor progeny RF could replicate at the high temperature. The data indicate that the block in DNA replication in the dnaB mutant involves the inability to perform semiconservative replication of double-stranded RF DNA. For determination of membrane-bound DNA, cells were lysed by lysozyme treatment in the presence of 15% (w/w) sucrose at 00 for 30 min, followed by 3-4 cycles of freezethawing. The lysate was then analyzed by centrifugation in a 20-40% sucrose gradient over a shelf of 60% sucrose (3).Preparation of 82P-labeled M13 phage and 3H-labeled ss M13 DNA and radioactivity assays have been described (4). RESULTS Abortive infection at 410To detect abortive infection under the conditions used, E. coli HfrH 165/70 (dnaB) cells were infected with M13 both at the permissive (340) and restrictive (410)

show abstract

mentioning

confidence: 99%

Replication of Bacteriophage M13: Specificity of the Escherichia coli dna B Function for Replication of Double-Stranded M13 DNA

Olsen¹,

Staudenbauer²,

Hofschneider³

1972

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…There is mounting evidence that a transcriptional event is required for DNA synthesis in coliphages X (32) and M13 (33,34), in colicin E1 (35), and in F-factor (36) as well as in E. coli (37). On the basis of our data we suggest that the highly specific P4 transcribing enzyme recognizes the replication origin of P4 DNA and synthesizes a polyribonucleotide that serves as the primer for DNA synthesis.…”

Section: Discussionmentioning

confidence: 72%

A Transcribing Activity Induced by Satellite Phage P4

Barrett

Gibbs

Calendar

1972

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Je me suis jointà l'effort initié par Brutlag et Kornberg d'abordà propos de la réplication de l'ADN de M13 puis, grâceà la réaction acellulaire mise au point par Brutlag, j'ai montré que la formation du double brin du ϕX174était insensiblè a la drogue qui bloque l'ARN polymérase standard, ce qui suggérait l'existence d'une ARN polymérase alternative pour la synthèse du primer Wickner et al, 1972).À l'Université de Harvard David Denhardt, avec qui j'avais travaillé uń eté, avait rapporté que la formation du double brin du ϕX174 dépendait du gène dnaB d'E. coli.…”

Section: Société De Biologie De Parisunclassified