RNA Synthesis by in Vitro Selected Ribozymes for Recreating an RNA World

Martin, Lyssa L.; Unrau, Peter J.; Müller, Ulrich F.

doi:10.3390/life5010247

Cited by 33 publications

(27 citation statements)

References 95 publications

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“…[1][2][3][4] This RNA world hypothesis is supported by the ability to explain how an interdependent DNA-RNA-protein system could have originated from a prebiotic environment, 4 by the identification of 'molecular fossils' in extant life forms, [5][6][7][8] and by the in vitro selection of RNA molecules that can catalyze chemical reactions required for an RNA world. 9,10 However, direct evidence of RNA world organisms may never be found because they existed more than 3.4 billion years ago, when apparently modern life forms left traces in the fossil record. 11 Instead, it may be possible to test how RNA world organisms could have functioned by trying to generate RNA world organisms in the lab, from prebiotically plausible compounds.…”

Section: Introductionmentioning

confidence: 99%

Lower temperature optimum of a smaller, fragmented triphosphorylation ribozyme

Akoopie

Müller

2016

Phys. Chem. Chem. Phys.

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The RNA world hypothesis describes a stage in the early evolution of life in which catalytic RNAs mediated the replication of RNA world organisms. One challenge to this hypothesis is that most existing ribozymes are much longer than what may be expected to originate from prebiotically plausible methods, or from the polymerization by currently existing polymerase ribozymes. We previously developed a 96-nucleotide long ribozyme, which generates a chemically activated 5'-phosphate (a 5'-triphosphate) from a prebiotically plausible molecule, trimetaphosphate, and an RNA 5'-hydroxyl group. Analogous ribozymes may have been important in the RNA world to access an energy source for the earliest life forms. Here we reduce the length of this ribozyme by fragmenting the ribozyme into multiple RNA strands, and by successively removing its longest double strand. The resulting ribozyme is composed of RNA fragments with none longer than 34 nucleotides. The temperature optimum was ∼20 °C, compared to ∼40 °C for the parent ribozyme. This shift in temperature dependence may be a more general phenomenon for fragmented ribozymes, and may have helped RNA world organisms to emerge at low temperature.

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Section: Introductionmentioning

confidence: 99%

Lower temperature optimum of a smaller, fragmented triphosphorylation ribozyme

Akoopie

Müller

2016

Phys. Chem. Chem. Phys.

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“…Martin y Müller (2015) definen que "un nucleótido es la fusión de tres componentes discretos: un grupo fosfato, un azúcar de ribosa y una base nitrogenada" (p. 251), de estos tres componentes el que conlleva mayor importancia y dificultad de síntesis son las bases nitrogenadas (Guanina, citosina, Uracilo y Adenina). Juan Oró (1961) sintetizó "adenina mediante una reacción con amoniaco y cianuro de hidrógeno la cual tuvo un rendimiento de 0,5%" (p. 1193-1194), luego otros científicos sintetizaron citosina y uracilo.…”

Section: Teorías Científicas Del Origen De La Vidaunclassified

Evaluación científica a las teorías del origen de la vida e inclusión del modelo bíblico como una explicación alternativa

García¹,

Hernández²,

García³

et al. 2017

Apunt. univ.

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ResumenEl objetivo de esta investigación consiste en evaluar de forma sucinta las principales teorías del origen de la vida y proponer razones puntuales para considerar el relato bíblico como una alternativa confiable respecto a los orígenes. La investigación tiene un enfoque cualitativo de carácter descriptivo. Para la recolección de datos se emplea la técnica de fichaje bibliográfico, que permite organizar el estado del arte de las teorías del origen de la vida y del relato del Génesis. Diversos autores y líneas investigativas enlistan características que se relacionan con la aparición de la vida, pero no existe una definición clara de vida en la comunidad científica. Las teorías del mundo del RNA, la panspermia, y las membranas prebiológicas abordan el origen de la vida desde un enfoque abiogénico, gradualista y evolutivo. Por otro lado, en la protología del Antiguo Cercano Oriente se encuentra el relato del Génesis bíblico como una narrativa histórica, secuencial y amítica, que tiene características diferenciadoras respecto a las cosmogonías sincrónicas del Enuma Elish, Eridu Genesis y Atrahasis. En conclusión, las teorías actuales del origen de la vida comportan limitaciones significativas en su abordaje, lo cual dilucida la posibilidad de considerar el texto Bíblico de la creación como una teoría alternativa al origen de la vida.Palabras clave: Vida, Abiogénesis, Protología, teorías científicas, origen, Cosmogonía. AbstractThe purpose of this research is to succinctly evaluate the main theories of the origin of life and to propose specific reasons to consider the biblical story as a reliable alternative to the origins. The research has a qualitative approach of descriptive character. For the data colletion is used the technique of bibliographic transfer, which allows to organize the state of the art of theories of the origin of life and the Genesis account. Several authors and research lines to list characteristics that 1 Correspondencia al autor

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“…Short RNA nucleotide sequences have an intrinsic ability to fold back on themselves to form the tools essential for life: the RNA hairpin with its typical base-paired stems and the loop which is constituted by a single-stranded RNA nucleotide sequence (Moore, 1999;Hendrix et al, 2005;Gwiazda et al, 2012;Martin et al, 2015) both constituted in a repeat syntax. While the double-stranded stems are not very active in binding to RNAs that are not part of the stem, the loops are open for binding events to other RNA such as loops from both self and non-self RNAs (Smit et al, 2006).…”

Section: Crucial Steps To Life: From Abiotic Chemistry To Biotic Codementioning

confidence: 99%

Crucial steps to life: From chemical reactions to code using agents

Witzany¹

2016

Biosystems

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a b s t r a c tThe concepts of the origin of the genetic code and the definitions of life changed dramatically after the RNA world hypothesis. Main narratives in molecular biology and genetics such as the "central dogma," "one gene one protein" and "non-coding DNA is junk" were falsified meanwhile. RNA moved from the transition intermediate molecule into centre stage. Additionally the abundance of empirical data concerning nonrandom genetic change operators such as the variety of mobile genetic elements, persistent viruses and defectives do not fit with the dominant narrative of error replication events (mutations) as being the main driving forces creating genetic novelty and diversity. The reductionistic and mechanistic views on physico-chemical properties of the genetic code are no longer convincing as appropriate descriptions of the abundance of non-random genetic content operators which are active in natural genetic engineering and natural genome editing.

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RNA Synthesis by in Vitro Selected Ribozymes for Recreating an RNA World

Cited by 33 publications

References 95 publications

Lower temperature optimum of a smaller, fragmented triphosphorylation ribozyme

Lower temperature optimum of a smaller, fragmented triphosphorylation ribozyme

Evaluación científica a las teorías del origen de la vida e inclusión del modelo bíblico como una explicación alternativa

Crucial steps to life: From chemical reactions to code using agents

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