RNA silencing of hormonal biosynthetic genes impairs larval growth and development in cotton bollworm, Helicoverpa armigera

Jaiwal, Anjali; Natarajaswamy, Kalleda; Rajam, Manchikatla Venkat

doi:10.1007/s12038-020-00079-6

Cited by 3 publications

(2 citation statements)

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“…Numerous studies have shown that the insect brain, as the receptor and programmable center, controls diapause by secreting endocrine hormones. [7][8][9][10] For example, pupal diapause in H. armigera is due to a failure in the synthesis and release of a prothoracicotropic hormone (PTTH) in the brain, resulting in further failure to stimulate the prothoracic glands to produce ecdysteroids that promote pupal-adult development. 11 Furthermore, some works have demonstrated the equally important role of insulin signaling in diapause.…”

Section: Introductionmentioning

confidence: 99%

Histone acetylation is associated with pupal diapause in cotton bollworm, Helicoverpa armigera

Lu,

Li,

Liao

et al. 2023

Pest Management Science

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BACKGROUNDDiapause is an environmentally preprogrammed period of arrested development that is important to insect survival and population growth. Histone acetylation, an epigenetic modification, has several biological functions, but its role in agricultural pest diapause is unknown. In this study, we investigated the role of histone H3 acetylation in the diapause of Helicoverpa armigera.RESULTSThe histone H3 gene of H. armigera was cloned, and multiple sequence alignment of amino acids revealed that the potential lysine acetylation sites were highly conserved across species. Investigation of histone H3 acetylation levels in diapause‐ and nondiapause‐type pupae showed that acetylation levels were down‐regulated in diapause‐type pupae and were lower in diapausing pupae compared to nondiapause pupae. By screening the genome, six histone acetyltransferase (HAT) and eight histone deacetylase (HDAC) genes responsible for antagonizing catalytic histone acetylation modifications were identified in H. armigera, and most of them exhibited different expression patterns between diapause‐ and nondiapause‐type pupae. To elucidate the effect of histone H3 acetylation on diapause in H. armigera, the diapause pupae were injected with the histone acetylation activator trichostatin A (TSA). The results indicated that TSA injection increased the levels of histone H3 acetylation, causing the diapausing pupae to revert to development. Furthermore, transcriptome analysis revealed that 259 genes were affected by TSA injection, including genes associated with metabolism, resistance, and immunological responses.CONCLUSIONThese results suggest that histone acetylation is inseparably related to the pupal diapause of H. armigera, which promises to be a potential target for pest control. © 2023 Society of Chemical Industry.

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Section: Introductionmentioning

confidence: 99%

Histone acetylation is associated with pupal diapause in cotton bollworm, Helicoverpa armigera

Lu,

Li,

Liao

et al. 2023

Pest Management Science

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“…The juvenile hormone acid-O-methyl transferase (JHAMT) is characterized as a key regulatory enzyme playing a pivotal role during insect metamorphosis and reproduction physiology by catalyzing the final step of SAMdependent methylation of JH-acids into their cognate methyl esters. RNAi-mediated silencing or knockdown of JHAMT in different insects have reported the impaired larval development, including precocious metamorphosis and adults with poor reproduction ability [5][6][7]. Helicoverpa armigera L. Hubner is a polyphagous pest on several host species such as cotton, tomato, black gram, soybean, ladyfinger, etc.…”

Section: Introductionmentioning

confidence: 99%

Identification of Small-Molecule Potential Inhibitor(s) for <em>Helicoverpa armigera</em> Juvenile Hormone Acid-o-Methyl Transferase (HaJHAMT) through Molecular Docking and MD Simulation Approaches

Sharma

Kumar

Das

et al. 2021

Proceedings of the 1st International Electronic Conference on Entomology

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Juvenile hormone compounds belong to a family of acyclic sesquiterpenoids, biosynthesized within small paired endocrine glands of corpora allata-corpora cardiaca having a neural connection with the brain. Stringent regulation of JH-levels in insect haemolymph is one of the most critical factors ensuring normal growth and development in insects. JHAMT is characterized as a key regulatory enzyme playing a pivotal role during insect metamorphosis and reproduction physiology by catalyzing the final step of SAM-dependent methylation of JH-acids into their cognate methyl esters. Recent reports on biochemical and molecular characterization of JHAMT gene from different insect species have proposed the JHAMT enzymatic activity as a potential target for the development of novel small molecule insect growth regulator(s). Therefore, in the present study, the protein sequence identity dependent homology model for JHAMT protein from a polyphagous pest, Helicoverpa armigera (order: Lepidoptera) has been generated and subsequently explored for virtual screening against small molecules natural product library. One such promising compouns namely, Scolimoside (HMDB05799) showing interactions with amino acid residues of enzyme's substrate-binding pocket (SBP) was further validated using molecular docking and Molecular Dynamics simulation (MD simulation). The study may pave the way forward for the design and development of novel eco-friendly small regulatory molecules as a component of integrated pest management.

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Functional Characterization of Abdominal-A in the Pine Caterpillar Moth, Dendrolimus punctatus

Liu,

Chen,

et al. 2024

Forests

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Hox genes, specifically the bithorax complex (ubx, abdominal-a, and abdominal-b), play a crucial role in specifying posterior abdominal development and serve as key regulators of germline gene development in insects. However, the function of the bithorax complex in the pine caterpillar moth, Dendrolimus punctatus, a major pine tree defoliator in China, remains largely unknown. Specifically, Abdominal-A (Abd-a) controls regional variation in abdominal segmentation in model insects such as Drosophila and Tribolium; however, its role in D. Punctatus remains unexplored. In this study, CRISPR/Cas9 was used to functionally characterize Abd-a in D. punctatus. Two target sites were selected, and the genotypes and phenotypes of the G0 and G1 generations were evaluated. Our findings indicate that knocking out Abd-a led to an abnormality in the posterior segments A2–A7, as well as the loss of appendages, mainly prolegs, and affected the thoracic T3 segmentation as well as wing development. Moreover, mutation in Abd-a also impacted anal and reproductive development. Taken together, these results demonstrate that DpAbd-a is essential for embryonic and reproductive development in D. punctatus and could be a promising target for genetic control of this devastating conifer defoliator.

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RNA silencing of hormonal biosynthetic genes impairs larval growth and development in cotton bollworm, Helicoverpa armigera

Cited by 3 publications

References 47 publications

Histone acetylation is associated with pupal diapause in cotton bollworm, Helicoverpa armigera

Histone acetylation is associated with pupal diapause in cotton bollworm, Helicoverpa armigera

Identification of Small-Molecule Potential Inhibitor(s) for <em>Helicoverpa armigera</em> Juvenile Hormone Acid-o-Methyl Transferase (HaJHAMT) through Molecular Docking and MD Simulation Approaches

Functional Characterization of Abdominal-A in the Pine Caterpillar Moth, Dendrolimus punctatus

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RNA silencing of hormonal biosynthetic genes impairs larval growth and development in cotton bollworm, Helicoverpa armigera

Cited by 3 publications

References 47 publications

Histone acetylation is associated with pupal diapause in cotton bollworm, Helicoverpa armigera

Histone acetylation is associated with pupal diapause in cotton bollworm, Helicoverpa armigera

Identification of Small-Molecule Potential Inhibitor(s) for &lt;em&gt;Helicoverpa armigera&lt;/em&gt; Juvenile Hormone Acid-o-Methyl Transferase (HaJHAMT) through Molecular Docking and MD Simulation Approaches

Functional Characterization of Abdominal-A in the Pine Caterpillar Moth, Dendrolimus punctatus

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Identification of Small-Molecule Potential Inhibitor(s) for <em>Helicoverpa armigera</em> Juvenile Hormone Acid-o-Methyl Transferase (HaJHAMT) through Molecular Docking and MD Simulation Approaches