RNA-sequencing study of peripheral blood monocytes in chronic periodontitis

Abstract

Background Monocytes are an important cell type in chronic periodontitis (CP) by interacting with oral bacteria and mediating host immune response. The aim of this study was to reveal new functional genes and pathways for CP at monocyte transcriptomic level. Methods We performed an RNA-sequencing (RNA-seq) study of peripheral blood monocytes (PBMs) in 5 non-smoking moderate to severe CP (case) individuals vs. 5 controls. We took advantage of a microarray study of periodontitis to support our findings. We also performed pathway-based analysis on the identified differentially expressed (DEx) transcripts/isoforms using DAVID (Database for Annotation, Visualization and Integrated Discovery). Results Through differential expression analyses at both whole gene (or whole non-coding RNA) and isoform levels, we identified 380 DEx transcripts and 5,955 DEx isoforms with a PPEE (posterior probability of equal expression) of < 0.05. Pervasive up-regulation of transcripts at isoform level in CP vs. control individuals were observed, suggesting a more functionally active monocyte transcriptome for CP. By comparing with the microarray dataset, we identified several CP-associated novel genes (e.g., FACR and CUX1) that have functions to interact with invading microorganisms or enhance TNF production on lipopolysaccharide stimulation. DAVID analysis of both the RNA-seq and the microarray datasets leads to converging evidence supporting “endocytosis”, “cytokine production” and “apoptosis” as significant biological processes in CP. Conclusions As the first RNA-seq study of PBMs for CP, this study provided novel findings at both gene (e.g., FCAR and CUX1) and biological process level. The findings will contribute to better understanding of CP disease mechanisms.

S100A12 Expression Is Modulated During Monocyte Differentiation and Reflects Periodontitis Severity

“…As S100A12 gene expression is very sensitive to low levels of LPS (12), we speculate that higher exposure to LPS in periodontitis might be at least partially responsible for the enhanced S100A12 expression seen in this study. In fact, transcriptome analysis of circulating monocytes in periodontitis evidenced they might be more functionally active, with the identification of several genes that interact with invading microorganisms or respond to LPS stimulation (35).…”

Platelet‐rich fibrin/biphasic calcium phosphate impairs osteoclast differentiation and promotes apoptosis by the intrinsic mitochondrial pathway in chronic periodontitis

“…Lundmark et al . reported the expression of apoptosis‐related genes in gingival tissues, and Liu et al . demonstrated that apoptotic proteins were upregulated in peripheral blood monocytes of patients with chronic periodontitis by DNA microarray studies.…”

“…39 Kasprzak et al 40 demonstrated the greater expression of apoptotic protein Bcl-2 in gingival tissues of patients with chronic periodontitis. Lundmark et al 41 reported the expression of apoptosisrelated genes in gingival tissues, and Liu et al 42 demonstrated that apoptotic proteins were upregulated in peripheral blood monocytes of patients with chronic periodontitis by DNA microarray studies.…”

Identification of miR-708-5p in peripheral blood monocytes: Potential marker for postmenopausal osteoporosis in Mexican-Mestizo population

“…To begin with, the sample size used in this work was somewhat little although comparable to other publications using next-generation sequencing (RNA-seq) in other diseases and circulating monocytes. 15,60,61 Consequently, we were only able to identify one miRNA showing clear changes between groups. Therefore, other miRNAs with slighter changes in their expression might have not been identified.…”