RNA sequencing of kidney and liver transcriptome obtained from wild cynomolgus macaque (Macaca fascicularis) originating from Peninsular Malaysia

Abstract

ObjectiveUsing high-throughput RNA sequencing technology, this study aimed to sequence the transcriptome of kidney and liver tissues harvested from Peninsular Malaysia cynomolgus macaque (Macaca fascicularis). M. fascicularis are significant nonhuman primate models in the biomedical field, owing to the macaque’s biological similarities with humans. The additional transcriptomic dataset will supplement the previously described Peninsular Malaysia M. fascicularis transcriptomes obtained in a past endeavour.ResultsA total of 75,350,240 sequence reads were obtained via Hi-seq 2500 sequencing technology. A total of 5473 significant differentially expressed genes were called. Gene ontology functional categorisation showed that cellular process, catalytic activity, and cell part categories had the highest number of expressed genes, while the metabolic pathways category possessed the highest number of expressed genes in the KEGG pathway analysis. The additional sequence dataset will further enrich existing M. fascicularis transcriptome assemblies, and provide a dataset for further downstream studies.Electronic supplementary materialThe online version of this article (10.1186/s13104-018-4014-1) contains supplementary material, which is available to authorized users.

Discovery of novel genic-SSR markers from transcriptome dataset of an important non-human primate, Macaca fascicularis

“…A transcriptome dataset was generated from a previous RNA sequencing project of the M . fascicularis on liver, kidney, lymph node, spleen and thymus 30,31 . We subjected the raw sequencing reads to quality assessment using FASTQC v0.11.2.…”

“…Hitherto, development of genic-SSR markers from whole transcriptome sequencing data of cynomolgus macaque has yet to be attempted. Therefore, the present study aimed to develop genic-SSR markers from an in-house transcriptome dataset of the Malaysian cynomolgus macaque generated from our previous studies 30,31 . This study is the first comprehensive report on the development of genic-SSR markers from the transcriptome of cynomolgus macaque.…”