RNA sequencing data of Notch ligand treated human dental pulp cells

Manokawinchoke, Jeeranan; Nattasit, Praphawi; Thongngam, Tanutchaporn; Pavasant, Prasit; Tompkins, Kevin; Egusa, Hiroshi; Osathanon, Thanaphum

doi:10.1016/j.dib.2018.01.058

Cited by 3 publications

(10 citation statements)

References 5 publications

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“…Further, the decrease expression of mRNA related to cell cycle was observed in Jagged1‐treated hPDLs compared with the control. Similarly, Jagged1‐treated human dental pulp cells (hDPs) also enhanced genes related to extracellular matrix pathway and attenuated genes involving in cell cycle regulation (Manokawinchoke, Nattasit et al, ; Manokawinchoke et al, ). The present study demonstrated that CDK1 and PLK1 were downregulated in Jagged1‐treated hPDLs.…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, tissue culture surfaces were incubated with recombinant protein G at a concentration of 50 μg/ml for 16 hr. After washing, surfaces were then incubated with bovine serum albumin (10 mg/ml) for 2 hr and Jagged1/Fc (10 nM) for 2 hr, respectively (Beckstead et al, ; Manokawinchoke, Nattasit et al, ; Manokawinchoke et al, ; Osathanon, Ritprajak et al, ). Human IgG Fc fragment (hFc) was employed in the control condition.…”

Section: Methodsmentioning

confidence: 99%

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Gene expression profiling of Jagged1‐treated human periodontal ligament cells

et al. 2019

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Objective: Jagged1 regulates several biological functions in human periodontal ligament cells (hPDLs). The present study aimed to evaluate mRNA expression profiling of Jagged1-treated hPDLs using microarray technique. Methods:Notch ligands, Jagged1, were indirectly immobilized on tissue culture surface. Subsequently, hPDLs were seeded on Jagged1 immobilized surface and maintained in growth medium for 48 hr. Total RNA was collected and processed. Gene expression profiling was examined using microarray technique. Real-time polymerase chain reaction and immunofluorescence staining were employed to determine mRNA and protein expression levels, respectively. Cell proliferation and colony-forming unit assay were performed. Cell cycle was evaluated using propidium iodide staining and flow cytometry analysis. Results:The isolated cells demonstrated fibroblast-like morphology and exhibited the co-expression of CD44, CD90, and CD105 surface markers. After stimulated with Jagged1, the total of 411 genes was differentially expressed, consisting both coding and non-coding genes. For coding genes, 165 and 160 coding genes were upregulated and downregulated, respectively. Pathway analysis revealed that the upregulated genes were mainly involved in cellular interactions, signal transduction, and collagen formation and degradation while the downregulated genes were in the events and phases in cell cycle. Jagged1 significantly decreased cell proliferation, reduced colony-forming unit ability, and induced G0/G1 cell cycle arrest in hPDLs.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Gene expression profiling of Jagged1‐treated human periodontal ligament cells

et al. 2019

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“…Cell viability was evaluated using MTT assay. 46 We used a standard cell line toxicity test to appreciate the cell viability. In this assay, we maintained a mouse fibroblast cell line L929 in DMEM (Sigma-Aldrich) containing 10% FBS, l -glutamine, penicillin, and streptomycin at 37 °C in humidified air containing 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

“…Cell viability was evaluated using MTT assay . We used a standard cell line toxicity test to appreciate the cell viability.…”

Section: Methodsmentioning

confidence: 99%

Electrospun Poly(ε-caprolactone) Fiber Scaffolds Functionalized by the Covalent Grafting of a Bioactive Polymer: Surface Characterization and Influence on in Vitro Biological Response

et al. 2019

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The purpose of this study is to present the poly(caprolactone) (PCL) functionalization by the covalent grafting of poly(sodium styrene sulfonate) on electrospun scaffolds using the “grafting from” technique and evaluate the effect of the coating and surface wettability on the biological response. The “grafting from” technique required energy (thermal or UV) to induce the decomposition of the PCL (hydro)peroxides and generate radicals able to initiate the polymerization of NaSS. In addition, UV irradiation was used to initiate the radical polymerization of NaSS directly from the surface (UV direct “grafting from”). The interest of these two techniques is their easiness, the reduction of the number of process steps, and its applicability to the industry. The selected parameters allow controlling the grafting rate (i.e., degree of functionalization). The aim of the study was to compare two covalent grafting in terms of surface functionalization and hydrophilicity and their effect on the in vitro biological responses of fibroblasts. The achieved results showed the influence of the sulfonate functional groups on the cell response. In addition, outcomes highlighted that the UV direct “grafting from” method allows to moderate the amount of sulfonate groups and the surface hydrophilicity presents a considerable interest for covalently immobilizing bioactive polymers onto electrospun scaffolds designed for tissue engineering applications using efficient post-electrospinning chemical modification.

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“…Immobilized Jagged1-treated human dental pulp cells (hDPCs) exhibited the upregulation of genes that are involved in ECM components as well as ECM-receptor interaction and focal adhesion-related pathways, such as ECM organization, collagen biosynthesis, and modification of enzymes (Manokawinchoke et al, 2017). In addition, Jagged1 plays a pivotal role in the cell-matrix interaction (Nehring et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Effects of decellularized extracellular matrix derived from Jagged1-treated human dental pulp stem cells on biological responses of stem cells isolated from apical papilla

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Fournier

et al. 2022

Front. Cell Dev. Biol.

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Objective: Indirect Jagged1 immobilization efficiently activates canonical Notch signaling in human dental pulp stem cells (hDPSCs). This study aimed to investigate the characteristics of the Jagged1-treated hDPSC-derived decellularized extracellular matrix (dECM) and its biological activity on odonto/osteogenic differentiation of stem cells isolated from apical papilla (SCAPs).Methods: Bioinformatic database of Jagged1-treated hDPSCs was analyzed using NetworkAnalyst. hDPSCs seeded on the Jagged1 immobilized surface were maintained with normal or osteogenic induction medium (OM) followed by decellularization procedure, dECM-N, or dECM-OM, respectively. SCAPs were reseeded on each dECM with either the normal medium or the OM. Cell viability was determined by MTT assay. Characteristics of dECMs and SCAPs were evaluated by SEM, EDX, immunofluorescent staining, and alcian blue staining. Mineralization was assessed by alizarin red S, Von Kossa, and alkaline phosphatase staining. Statistical significance was considered at p < 0.05.Results: RNA-seq database revealed upregulation of several genes involved in ECM organization, ECM–receptor interaction, and focal adhesion in Jagged1-treated hDPSCs. Immobilized Jagged1 increased the osteogenesis of the hDPSC culture with OM. dECMs showed fibrillar-like network structure and maintained major ECM proteins, fibronectin, type I-collagen, and glycosaminoglycans. A decrease in calcium and phosphate components was observed in dECMs after the decellularized process. Cell viability on dECMs did not alter by 7 days. Cell attachment and f-actin cytoskeletal organization of SCAPs proliferated on Jagged1-treated dECMs were comparable to those of the control dECMs. SCAPs exhibited significantly higher mineralization on dECM-N in OM and markedly enhanced on dECM-OM with normal medium or OM conditions.Conclusion: Jagged1-treated hDPSC-derived dECMs are biocompatible and increase odonto/osteogenic differentiation of SCAPs. The results suggested the potential of Jagged1 dECMs, which could be further developed into ECM scaffolds for application in regenerative medicine.

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RNA sequencing data of Notch ligand treated human dental pulp cells

Cited by 3 publications

References 5 publications

Gene expression profiling of Jagged1‐treated human periodontal ligament cells

Gene expression profiling of Jagged1‐treated human periodontal ligament cells

Electrospun Poly(ε-caprolactone) Fiber Scaffolds Functionalized by the Covalent Grafting of a Bioactive Polymer: Surface Characterization and Influence on in Vitro Biological Response

Effects of decellularized extracellular matrix derived from Jagged1-treated human dental pulp stem cells on biological responses of stem cells isolated from apical papilla

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