RNA-seq transcriptomic analysis of green tea polyphenols regulation of differently expressed genes in Saccharomyces cerevisiae under ethanol stress

Liu, Cheng; Zhang, Xin; Zheng, Xiaojie; Wu, Zufang; Weng, Peifang

doi:10.1007/s11274-019-2639-4

Cited by 16 publications

(17 citation statements)

References 30 publications

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“…Secondly, according to our previous experimental method, the C. paliurus leaf powder was extracted with distilled water at a liquid/material ratio (v/w) of 35 : 1 at 90°C for 70 min, and centrifuged at 6000 g for 8 min, collected the supernatant, and then repeated the extraction of the insoluble residue produced by the above method again. The two supernatants collected were mixed according to the method we previously reported, and purified using a polyamide column (Cheng et al 2019a(Cheng et al , 2019b. Finally, the obtained filtrate containing CPF was concentrated by a rotary evaporator and lyophilized.…”

Section: Preparation Of Cpfmentioning

confidence: 99%

“…The E. faecalis 131-2 cells were cultured in incubator at 37°C and monitored by measuring the optical density of the culture medium at 600 nm (OD 600 ). After 12 h cultivation, cells were collected for SEM analysis and RNA-seq analysis (Cheng et al 2019a(Cheng et al , 2019b. All samples were stored at À80°C before use.…”

Section: Preparation Of Cpfmentioning

confidence: 99%

“…As a prebiotic candidate, C. paliurus flavonoids (CPF) has great potential to regulate gut microbiota and influence metabolic pathways (Xie et al 2018). The CPF can significantly improve the composition and structural imbalance of intestinal flora caused by high-fat diet and decreased the ratio of Firmicutes/Bacteroidetes (Cheng et al 2019a(Cheng et al , 2019b. However, there are still some gaps in the research on the influence of CPF on the microbiota under external pressure stress conditions.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Cyclocarya paliurus flavonoids modulation on the physiology and gene expression in Enterococcus faecalis under bile salt stress

Yan

Zhang

2022

Letters in Applied Microbiology

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Significance and Impact of the Study: This study will further deepen our understanding of the functional mechanism of CPF assisted Enterococcus faecalis 131-2 (E. faecalis 131-2) in the gastrointestinal environment. The result showed that many genes related to cell process, metabolic process and transport activity were significantly differentially expressed under bile salt stress (P < 0Á05), while the intervention of Cyclocarya paliurus flavonoids (CPF) could alleviate the occurrence of negative phenomena to a certain extent. At the same time, CPF effectively reduces the significant inhibition of the bile salt stress to the exponential growth of E. faecalis 131-2. This will provide a more comprehensive perspective for the future research of E. faecalis 131-2.

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Section: Preparation Of Cpfmentioning

confidence: 99%

Section: Preparation Of Cpfmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of Cyclocarya paliurus flavonoids modulation on the physiology and gene expression in Enterococcus faecalis under bile salt stress

Yan

Zhang

2022

Letters in Applied Microbiology

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“…Massive information from OMICs, and network analysis revealed that the EtOH stress response in yeast is a complex mechanism involving several pathways. For instance, the transcriptome, proteome, and metabolome landscapes revealed imbalances of many processes associated with stress responses (homeostasis, heat shock proteins, and redox balance), cell structures (plasma membrane components, and vacuolar functions), metabolisms (oxidative stress, amino acids, energy, flavoproteins, carbon, volatile compounds, polyphenols, TCA cycle, and L-aspartate), signal transduction, and RNA and protein synthesis (Stanley et al 2010b; Lourenço et al 2013; Lewis et al 2014; Santos et al 2017; Cheng et al 2019; Li et al 2019a). Disrupting regulatory network by mutating stress response transcription factors showed that glycolysis is related to EtOH tolerance (Liang et al 2021).…”

Section: Introductionmentioning

confidence: 99%

The ethanol tolerance in Saccharomyces cerevisiae under a phenomics perspective

Wolf

Marques

Almeida

et al. 2021

Preprint

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Ethanol (EtOH) is a substantial stressor for Saccharomyces cerevisiae. Data integration from strains with different phenotypes, including EtOH stress-responsive lncRNAs, are still not available. We covered these issues seeking systems modifications that drive the divergences between higher (HT) and lower (LT) EtOH tolerant strains under their highest stress conditions. We showed that these phenotypes are neither related to high viability nor faster population rebound after stress relief. LncRNAs work on many stress-responsive systems in a strain-specific manner promoting the EtOH tolerance. Cells use membraneless RNA/protein storage and degradation systems to endure the stress harming, and lncRNAs jointly promote EtOH tolerance. CTA1 and longevity are primer systems promoting phenotype-specific gene expression. The lower cell viability and growth under stress is a byproduct of sphingolipids and inositol phosphorylceramide dampening, acerbated in HTs by sphinganine, ERG9, and squalene overloads; LTs diminish this harm by accumulating inositol 1-phosphate. The diauxic shift drives an EtOH buffering by promoting an energy burst under stress, mainly in HTs. Analysis of mutants showed genes and lncRNAs in three strains critical for their EtOH tolerance. Finally, longevity, peroxisome, energy and lipid metabolisms, RNA/protein degradation and storage systems are the main pathways driving the EtOH tolerance phenotypes.

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“…In the response against oxidative stress, melatonin acts as a direct antioxidant by scavenging ROS and as an indirect antioxidant by decreasing oxidized glutathione and activating genes involved in the oxidative stress response, such as catalase, SOD, glutathione/glutaredoxin, and thioredoxin, leading to reduced lipid peroxidation and higher tolerance to H 2 O 2 [ 40 , 42 ]. Moreover, the accumulation of some compounds in the media, such as polyphenols or amino acids, has been related to increased yeast tolerance to ethanol [ 47 , 48 , 49 , 50 , 51 , 52 ]. Resveratrol has been the most studied polyphenol, showing that this compound is able to increase yeast tolerance to ethanol by decreasing lipid peroxidation and SOD activity and by regulating the membrane composition [ 49 ].…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Melatonin on Saccharomyces cerevisiae under Ethanol Stress

et al. 2021

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During alcoholic fermentation, Saccharomyces cerevisiae is subjected to several stresses, among which ethanol is of capital importance. Melatonin, a bioactive molecule synthesized by yeast during alcoholic fermentation, has an antioxidant role and is proposed to contribute to counteracting fermentation-associated stresses. The aim of this study was to unravel the protective effect of melatonin on yeast cells subjected to ethanol stress. For that purpose, the effect of ethanol concentrations (6 to 12%) on a wine strain and a lab strain of S. cerevisiae was evaluated, monitoring the viability, growth capacity, mortality, and several indicators of oxidative stress over time, such as reactive oxygen species (ROS) accumulation, lipid peroxidation, and the activity of catalase and superoxide dismutase enzymes. In general, ethanol exposure reduced the cell growth of S. cerevisiae and increased mortality, ROS accumulation, lipid peroxidation and antioxidant enzyme activity. Melatonin supplementation softened the effect of ethanol, enhancing cell growth and decreasing oxidative damage by lowering ROS accumulation, lipid peroxidation, and antioxidant enzyme activities. However, the effects of melatonin were dependent on strain, melatonin concentration, and growth phase. The results of this study indicate that melatonin has a protective role against mild ethanol stress, mainly by reducing the oxidative stress triggered by this alcohol.

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RNA-seq transcriptomic analysis of green tea polyphenols regulation of differently expressed genes in Saccharomyces cerevisiae under ethanol stress

Cited by 16 publications

References 30 publications

Analysis of Cyclocarya paliurus flavonoids modulation on the physiology and gene expression in Enterococcus faecalis under bile salt stress

Analysis of Cyclocarya paliurus flavonoids modulation on the physiology and gene expression in Enterococcus faecalis under bile salt stress

The ethanol tolerance in Saccharomyces cerevisiae under a phenomics perspective

Protective Effects of Melatonin on Saccharomyces cerevisiae under Ethanol Stress

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