RNA-Seq transcriptome data of undifferentiated and differentiated gonads of Siberian sturgeon

Klopp, Christophe; Lasalle, André; Landro, Santiago Di; Vizziano-Cantonnet, Denise

doi:10.1016/j.dib.2020.105741

Cited by 3 publications

(10 citation statements)

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“…foxl2 and 18S primers were validated in a previous work (Vizziano-Cantonnet et al, 2016). The primers used for cyp19a1, hsd17b1, irx3, irx5, and lhx2 are shown in Table 2 and were designed using the specific gene sequence obtained in the transcriptome study (Klopp et al, 2020;. obtained was analyzed using BioEdit software and blasted against the NCBI database (https://blast.ncbi.nlm.nih.gov/Blast.cgi) and our transcriptome database to confirm sequence identity (Klopp et al, 2020).…”

Section: Quantitative Pcrmentioning

confidence: 99%

Sex identification of morphologically‐undifferentiated Siberian sturgeon with statistical analysis of gene expression patterns

2021

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Sex identification is a key step in sex determination and differentiation research in fish. These processes are poorly understood in most fish species, partly due to the challenge of identifying sex prior to morphological differentiation. Precocious sex identification is difficult in part because there is no common master gene for sex determination among fish (Capel, 2017;Kikuchi & Hamaguchi, 2013), in contrast to eutherian mammals, which conserve the sexdetermining region Y (SRY) gene that triggers the male phenotype

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Section: Quantitative Pcrmentioning

confidence: 99%

Sex identification of morphologically‐undifferentiated Siberian sturgeon with statistical analysis of gene expression patterns

2021

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“…Primers for qPCR analysis were designed with the specific gene sequence obtained in the transcriptome study (Klopp et al, 2020) and validated by melting curve analysis (qPCR), agarose gel electrophoresis, and sequencing. The primers used are shown in Table 3.…”

Section: Quantitative Pcr (Qpcr)mentioning

confidence: 99%

“…The identities of some of these sequences were previously validated using phylogenetic approaches (amh, dmrt1, sox9, and cyp17a1, Berbejillo et al, 2012;cyp19a1, Lasalle Gerla, 2016). The other sequences were obtained from our gonadal transcriptome database (Klopp et al, 2020; and validated with BLASTp at the NCBI site (https://blast.ncbi.nlm.nih.…”

Section: Quantitative Pcr (Qpcr)mentioning

confidence: 99%

“…These contigs were analyzed phylogenetically to investigate their identity (see Results). gsdf and amh-r were searched in the Siberian sturgeon transcriptome databases (Klopp et al, 2020).…”

Section: Quantitative Pcr (Qpcr)mentioning

confidence: 99%

“…Multiple efforts have been made to identify the genes involved in gonadal differentiation in sturgeons (Berbejillo et al, 2012, 2013; Degani et al, 2019, 2022; Du et al, 2020; Fajkowska et al, 2019; Hale et al, 2010; Klopp et al, 2020; Lasalle et al, 2021; Okada et al, 2017; Vidotto et al, 2013; Vizziano‐Cantonnet et al, 2016; Vizziano‐Cantonnet, Lasalle, et al, 2018; Wang et al, 2019; Wuertz et al, 2018; Zhang et al, 2022). However, all of these works were performed using genetically‐unsexed fish.…”

Section: Introductionmentioning

confidence: 99%

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hsd17b1 is a key gene for ovarian differentiation of the Siberian sturgeon

Lasalle,

Benech‐Correa,

Brunet

et al. 2024

Molecular Reproduction Devel

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This is the first work using gonads from undifferentiated, genetically‐sexed Siberian sturgeon describing expression changes in genes related to steroid synthesis and female and male sex differentiation. One factor identified as relevant for ovarian differentiation was the gene coding for the enzyme Hsd17b1, which converts estrone into estradiol‐17β. hsd17b1 was highly activated in female gonads at 2.5 months of age, around the onset of sex differentiation, preceding activation of two other genes involved in estrogen production (cyp19a1 and foxl2). hsd17b1 was also strongly repressed in males. Two known foxl2 paralogs are found in Siberian sturgeon—foxl2 and foxl2l—but only foxl2 appeared to be associated with ovarian differentiation. With regard to the male pathway, neither 11‐oxygenated androgens nor classic male genes (amh, dmrt1, sox9, and dhh) were found to be involved in male sex differentiation, leaving open the question of which genes participate in early male gonad development in this ancient fish. Taken together, these results indicate an estrogen‐dependence of female sex differentiation and 11‐oxygenated androgen‐independence of male sex differentiation.

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Transcriptome Analyses Reveal Expression Profiles of Morphologically Undifferentiated and Differentiated Gonads of Yangtze Sturgeon Acipenser dabryanus

Ruan,

Li,

Yue

et al. 2023

Genes

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Sturgeon is known as a primitive fish with the ZZ/ZW sex determination system and is highly prized for its valuable caviar. Exploring the molecular mechanisms underlying gonadal differentiation would contribute to broadening our knowledge on the genetic regulation of sex differentiation of fish, enabling improved artificial breeding and management of sturgeons. However, the mechanisms are still poorly understood in sturgeons. This study aimed to profile expression patterns between female and male gonads at morphologically undifferentiated and early differentiated stages and identify vital genes involved in gonadal sex differentiation of sturgeons. The sexes of Yangtze sturgeon (Acipenser dabryanus) juveniles were identified via the sex-specific DNA marker and histological observation. Transcriptome analyses were carried out on female and male gonads at 30, 80 and 180 days post-hatching. The results showed that there was a total of 17 overlapped DEGs in the comparison groups of between female and male gonads at the three developmental stages, in which there were three DEGs related to ovarian steroidogenesis, including hsd17b1, foxl2 and cyp19a1. The three DEGs were highly expressed in the female gonads, of which the expression levels were gradually increased with the number of days after hatching. No well-known testis-related genes were found in the overlapped DEGs. Additionally, the expression levels of hsd17b1 and cyp19a1 mRNA were decreased with the knockdown of foxl2 mRNA via siRNA. The results further suggested that foxl2 should play a crucial role in the ovarian differentiation of sturgeons. In conclusion, this study showed that more genes involved in ovarian development than testis development emerged with sexually dimorphic expression during early gonadal sex differentiation, and it provided a preliminary understanding of the molecular regulation on gonadal differentiation of sturgeons.

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RNA-Seq transcriptome data of undifferentiated and differentiated gonads of Siberian sturgeon

Cited by 3 publications

References 2 publications

Sex identification of morphologically‐undifferentiated Siberian sturgeon with statistical analysis of gene expression patterns

Sex identification of morphologically‐undifferentiated Siberian sturgeon with statistical analysis of gene expression patterns

hsd17b1 is a key gene for ovarian differentiation of the Siberian sturgeon

Transcriptome Analyses Reveal Expression Profiles of Morphologically Undifferentiated and Differentiated Gonads of Yangtze Sturgeon Acipenser dabryanus

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